公开(公告)号：US20250034560A1

公开(公告)日：2025-01-30

申请号：US18689848

申请日：2022-09-06

Applicant: NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY ,  BEX CO., LTD.

Inventor： Ikuo NAKAMURA ,  So MAKABE ,  Yuichi KOBASHI ,  Yasuhiro MORIIZUMI

IPC: C12N15/11 ,  C12N9/22

Abstract: An object is to provide a nucleic acid sequence modifying composition and a method for modifying a target site of a nucleic acid sequence that do not depend on a PAM sequence or a PFS sequence. The object can be achieved by a nucleic acid sequence modifying composition including RNA and a fusion protein, in which the RNA includes a hybridization region that may hybridize to a sequence on the 5′ side or the 3′ side of a target site of a nucleic acid sequence, and a guide region that guides the fusion protein, the guide region includes a recognition region that forms a complex with the fusion protein, and the fusion protein includes a binding domain that recognizes the recognition region of the RNA and forms a complex with the recognition region and a modification domain that modifies the target site of the nucleic acid sequence.

公开(公告)号：US20250014777A1

公开(公告)日：2025-01-09

申请号：US18579839

申请日：2022-06-13

Applicant: BEX CO., LTD.

Inventor： Yusuke SAITO ,  So MAKABE ,  Yasuhiro MORIIZUMI

IPC: H01B5/00 ,  C12M1/42

Abstract: An object is to provide an electrode that is easily arranged at a predetermined position when electroporation is performed. The object is achieved by an electrode used for performing electroporation, the electrode includes: at least two or more conductors; and a member configured to hold each conductor and expose at least one end of each of the conductors to outside, the member has a first face where the one end of each of the conductors is exposed, and at least one or more protruding parts provided to the first face, and the length by which the one or more protruding parts protrude in an axis direction from the first face is longer than the length by which the one end of each of the conductors is exposed from the first face.

公开(公告)号：US20210292692A1

公开(公告)日：2021-09-23

申请号：US17262413

申请日：2019-07-19

Applicant: BEX CO., LTD.

Inventor： So MAKABE ,  Hiromichi WATANABE ,  Yasuhiro MORIIZUMI ,  Toshiyuki MORIIZUMI

IPC: C12M1/00 ,  C12N15/87 ,  C12M1/42

Abstract: Provided are a bubble ejection method based on a novel principle, a power supply device used for performing the bubble ejection method, and a bubble ejecting apparatus. A bubble can be ejected by a bubble ejection method for bubble ejection into a conductor, the bubble ejection method includes steps of: contacting a bubble generating electrode to a conductor; contacting a counter electrode to the conductor or a processing target; and applying a negative voltage to the bubble generating electrode, and a conductive material is exposed on at least a tip part of the bubble generating electrode.

公开(公告)号：US20180305654A1

公开(公告)日：2018-10-25

申请号：US15769259

申请日：2016-10-17

Applicant: BEX CO., LTD.

Inventor： Yoko YAMANISHI ,  Takuya KAMBAYASHI ,  Kazuki TAKAHASHI

IPC: C12M1/42 ,  G03F7/038 ,  C12N15/89 ,  G03F7/16 ,  G03F7/20 ,  G03F7/32

CPC classification number: C12M35/04 ,  C12M1/00 ,  C12N15/89 ,  G03F7/038 ,  G03F7/16 ,  G03F7/162 ,  G03F7/168 ,  G03F7/2002 ,  G03F7/26 ,  G03F7/322

Abstract: A bubble-jetting chip capable of jetting bubbles upward from a substrate is provided.Bubbles can be jetted upward from a substrate by a bubble-jetting chip comprising: at least a substrate, an energizing portion, and a bubble-jetting portion; the energizing portion being formed on the substrate; the bubble-jetting portion comprising an electrode that is formed of a conductive material, a shell part formed of an insulating photosensitive resin, and an extended section that extends from the shell part, the shell part covering a periphery of the electrode, the extended section extending beyond a tip of the electrode, and the bubble-jetting portion further comprising a space formed between the extended section and the electrode; and the electrode of the bubble-jetting portion being formed on the energizing portion.

公开(公告)号：US20180064073A1

公开(公告)日：2018-03-08

申请号：US15551791

申请日：2016-02-18

Applicant: Masakazu HASHIMOTO ,  Tokushima University ,  BEX Co., Ltd.

Inventor： Masakazu Hashimoto ,  Tatsuya Takemoto

IPC: A01K67/027 ,  C12N15/09 ,  C12N9/16

CPC classification number: A01K67/0275 ,  A01K67/027 ,  C12N9/16 ,  C12N15/09

Abstract: The disclosure relates to a method of introducing mRNA encoding Cas9 protein (Cas9 mRNA) into a mammalian embryo, comprising the steps of; (a) placing a mixture of the mammalian embryo and a solution comprising Cas9 mRNA in the gap between a pair of electrodes, and (b) applying a voltage to the electrodes for a voltage application duration, wherein the voltage and the voltage application duration achieve the efficiency of mRNA introduction (R) higher than the minimum required efficiency of mRNA introduction (Rmin) that is calculated on the basis of the concentration of Cas9 mRNA (ng/μl).