公开(公告)号：US20060019321A1

公开(公告)日：2006-01-26

申请号：US11172244

申请日：2005-06-29

Applicant: Jennifer Martinez ,  Basil Swanson ,  John Shively ,  Lin Li

Inventor： Jennifer Martinez ,  Basil Swanson ,  John Shively ,  Lin Li

IPC: G01N33/574 ,  C12M1/34

CPC classification number: B82Y30/00 ,  B82Y15/00 ,  G01N33/57473 ,  G01N2610/00 ,  Y10S436/805

Abstract: An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of CEA is described including injecting a possible CEA-containing sample into a sensor cell including the assay element, maintaining the sample within the sensor cell for time sufficient for binding to occur between CEA present within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

公开(公告)号：US20050244487A1

公开(公告)日：2005-11-03

申请号：US11072720

申请日：2005-03-04

Applicant: Jose-Maria Sansinena ,  Antonio Redondo ,  Basil Swanson ,  Chanel Yee ,  Annapoorna Sapuri/Butti ,  Atul Parikh ,  Calvin Yang

Inventor： Jose-Maria Sansinena ,  Antonio Redondo ,  Basil Swanson ,  Chanel Yee ,  Annapoorna Sapuri/Butti ,  Atul Parikh ,  Calvin Yang

IPC: A61K9/127

CPC classification number: A61K9/127 ,  A61K9/1277

Abstract: The present invention is directed to a process of forming a bilayer lipid membrane structure by depositing an organic layer having a defined surface area onto an electrically conductive substrate, removing portions of said organic layer upon said electrically conductive substrate whereby selected portions of said organic layer are removed to form defined voids within said defined surface area of said organic layer and defined islands of organic layer upon said electrically conductive substrate, and, depositing a bilayer lipid membrane over the defined voids and defined islands of organic layer upon said substrate whereby aqueous reservoirs are formed between said electrically conductive substrate and said bilayer lipid membrane, said bilayer lipid membrane characterized as spanning across the defined voids between said defined islands. A lipid membrane structure is also described together with an array of such lipid membrane structure.

Abstract translation: 本发明涉及一种通过将具有限定表面积的有机层沉积在导电基材上形成双层脂质膜结构的方法，在所述导电基材上除去所述有机层的部分，从而使所述有机层的选定部分为 被移除以在所述有机层的所述限定的表面区域内形成限定的空隙，并且在所述导电基底上限定有机层岛，并且在所述基底上沉积双层脂质膜超过限定的空隙和限定的有机层岛，由此水储存器为 形成在所述导电基底和所述双层脂质膜之间，所述双层脂质膜表征为横跨所述限定的岛之间的限定的空隙。 脂质膜结构也与这种脂质膜结构的阵列一起描述。

公开(公告)号：US20050078903A1

公开(公告)日：2005-04-14

申请号：US10958965

申请日：2004-10-04

Applicant: Karen Grace ,  Basil Swanson ,  Seppo Honkanen

Inventor： Karen Grace ,  Basil Swanson ,  Seppo Honkanen

IPC: G01N21/21 ,  G01N21/77 ,  G02B6/10 ,  G02B6/122 ,  G02B6/00

CPC classification number: G02B6/1221 ,  G01N21/21 ,  G01N21/7703 ,  G01N2021/7709 ,  G01N2021/7776 ,  G02B6/105

Abstract: The invention provides an apparatus and method for highly selective and sensitive chemical sensing. Two modes of laser light are transmitted through a waveguide, refracted by a thin film host reagent coating on the waveguide, and analyzed in a phase sensitive detector for changes in effective refractive index. Sensor specificity is based on the particular species selective thin films of host reagents which are attached to the surface of the planar optical waveguide. The thin film of host reagents refracts laser light at different refractive indices according to what species are forming inclusion complexes with the host reagents.

Abstract translation: 本发明提供了一种用于高选择性和敏感的化学感测的装置和方法。 激光的两种模式通过波导传输，由波导上的薄膜主体试剂涂层折射，并在相敏检测器中分析有效折射率的变化。 传感器的特异性是基于附着于平面光波导表面的主体试剂的特定种类的选择性薄膜。 主体试剂的薄膜根据与宿主试剂形成包合配合物的物质折射不同折射率的激光。

公开(公告)号：US20050027100A1

公开(公告)日：2005-02-03

申请号：US10629984

申请日：2003-07-29

Applicant: Jurgen Schmidt ,  Basil Swanson ,  Clifford Unkefer

Inventor： Jurgen Schmidt ,  Basil Swanson ,  Clifford Unkefer

IPC: B01J19/00 ,  C07K1/02 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/54353 ,  B01J19/0046 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731

Abstract: The present invention is directed to a method of forming an immobilized chemical moiety, such as a trifunctional chemical moiety that includes membrane-anchoring functionalities, on a solid support for use in automated chemical synthesis of a recognition molecule. The invention is further directed to a method of easily and efficiently synthesizing chemical moieties such as those used for biosensor applications. The invention is further directed to a composition, i.e., a solid support having a trifunctional chemical moiety linked thereto, that can be readily and easily used to generate biological molecules for applications, such as biosensor applications.

Abstract translation: 本发明涉及在用于识别分子的自动化学合成中的固体支持物上形成固定的化学部分例如包括膜锚定功能的三官能化学部分的方法。 本发明进一步涉及容易且有效地合成化学部分的方法，例如用于生物传感器应用的那些。 本发明进一步涉及一种组合物，即具有与其连接的三官能化学部分的固体支持物，其可以容易地和容易地用于产生用于应用的生物分子，例如生物传感器应用。

公开(公告)号：US20060019244A1

公开(公告)日：2006-01-26

申请号：US11172246

申请日：2005-06-29

Applicant: Jennifer Martinez ,  Basil Swanson ,  Karen Grace ,  Wyane Grace ,  Andrew Shreve

Inventor： Jennifer Martinez ,  Basil Swanson ,  Karen Grace ,  Wyane Grace ,  Andrew Shreve

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/554 ,  C12M1/34 ,  G01N33/569

CPC classification number: G01N21/648 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00725 ,  B01J2219/00743 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N33/54373 ,  G01N2610/00 ,  Y10S436/805

Abstract: An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of a biological target is described including injecting a biological target-containing sample into a sensor cell including the assay element, with the recognition ligands adapted for binding to selected biological targets, maintaining the sample within the sensor cell for time sufficient for binding to occur between selected biological targets within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting the fluorescent-label in any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

公开(公告)号：US20070141724A1

公开(公告)日：2007-06-21

申请号：US11590103

申请日：2006-10-30

Applicant: Jurgen Schmidt ,  Basil Swanson ,  Clifford Unkefer

Inventor： Jurgen Schmidt ,  Basil Swanson ,  Clifford Unkefer

IPC: C40B40/10 ,  C07K1/02

CPC classification number: B01J19/0046 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  C07K1/042 ,  G01N33/54353

Abstract: The present invention is directed to a method of forming an immobilized chemical moiety, such as a trifunctional chemical moiety that includes membrane-anchoring functionalities, on a solid support for use in automated chemical synthesis of a recognition molecule. The invention is further directed to a method of easily and efficiently synthesizing chemical moieties such as those used for biosensor applications. The invention is further directed to a composition, i.e., a solid support having a trifunctional chemical moiety linked thereto, that can be readily and easily used to generate biological molecules for applications, such as biosensor applications.

Abstract translation: 本发明涉及在用于识别分子的自动化学合成中的固体支持物上形成固定的化学部分例如包括膜锚定功能的三官能化学部分的方法。 本发明进一步涉及容易且有效地合成化学部分的方法，例如用于生物传感器应用的那些。 本发明进一步涉及一种组合物，即具有与其连接的三官能化学部分的固体支持物，其可以容易地和容易地用于产生用于应用的生物分子，例如生物传感器应用。

公开(公告)号：US20050191705A1

公开(公告)日：2005-09-01

申请号：US10790638

申请日：2004-03-01

Applicant: James Werner ,  Scott Reed ,  Basil Swanson

Inventor： James Werner ,  Scott Reed ,  Basil Swanson

IPC: G01N33/53 ,  G01N33/533 ,  G01N33/58

CPC classification number: G01N33/582 ,  G01N33/533

Abstract: An apparatus for and method of detecting a binding event between biomolecules is disclosed and includes admixing a target molecule including a first fluorophore and membrane vesicles including a trifunctional linker molecule, said trifunctional linker molecule including a second fluorophore, to form a sample, introducing a library of elements into said sample, each of said library elements having a binding affinity for said trifunctional linker molecule, and, screening said sample for fluorescence from said first fluorophore and said second fluorophore, such fluorescence indicative of a binding event between an element from said library of elements and said target molecule.

Abstract translation: 公开了用于检测生物分子之间的结合事件的装置和方法，包括混合包括第一荧光团的靶分子和包括三官能连接分子的膜泡囊，所述三官能连接分子包括第二荧光团，形成样品，引入文库 的元件进入所述样品，每个所述文库元件对所述三官能连接分子具有结合亲和力，并且从所述第一荧光团和所述第二荧光团筛选所述样品的荧光，所述荧光指示来自所述文库的元件之间的结合事件 的元素和所述靶分子。

公开(公告)号：US20080003694A1

公开(公告)日：2008-01-03

申请号：US11788183

申请日：2007-04-18

Applicant: Basil Swanson ,  Aaron Anderson ,  Jurgen Schmidt ,  Andrew Dattelbaum

Inventor： Basil Swanson ,  Aaron Anderson ,  Jurgen Schmidt ,  Andrew Dattelbaum

IPC: G01N33/48 ,  B32B9/04 ,  G01N33/566

CPC classification number: G01N33/54393 ,  G01N33/551 ,  Y10T428/31663

Abstract: The present invention provides a composite material including a substrate having an oxide surface, and, a continuous monolayer on the oxide surface, the monolayer including a silicon atom from a trifunctional alkyl/alkenyl/alkynyl silane group that attaches to the oxide surface, an alkyl/alkenyl/alkynyl portion of at least three carbon atoms, a polyalkylene glycol spacer group, and either a reactive site (e.g., a recognition ligand) or a site resistant to non-specific binding (e.g., a methoxy or the like) at the terminus of each modified SAM. The present invention further provides a sensor element, a sensor array and a method of sensing, each employing the composite material. Patterning is also provided together with backfilling to minimize non-specific binding.

Abstract translation: 本发明提供一种复合材料，其包括具有氧化物表面的基材和在氧化物表面上的连续单层，所述单层包含与氧化物表面连接的三官能烷基/烯基/炔基硅烷基团的硅原子，烷基 至少三个碳原子的链烯基/炔基部分，聚亚烷基二醇间隔基，以及反应性位点（例如，识别配体）或对非特异性结合（例如，甲氧基等）具有抗性的位点 每个修饰的SAM的末端。 本发明还提供了各自采用复合材料的传感器元件，传感器阵列和感测方法。 还提供图案化以及回填以最小化非特异性结合。

公开(公告)号：US20060003318A1

公开(公告)日：2006-01-05

申请号：US11132130

申请日：2005-05-17

Applicant: Basil Swanson ,  Xuedong Song ,  Clifford Unkefer ,  Louis Silks ,  Jurgen Schmidt

Inventor： Basil Swanson ,  Xuedong Song ,  Clifford Unkefer ,  Louis Silks ,  Jurgen Schmidt

IPC: C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/34 ,  G01N33/56983 ,  G01N2333/11 ,  Y10S435/808 ,  Y10S435/817 ,  Y10S436/805 ,  Y10S436/811 ,  Y10S436/815 ,  Y10T436/13 ,  Y10T436/200833

Abstract: A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain adapted for attachment to a substrate, (b) a fluorescent moiety capable of generating a fluorescent signal, and (c) a recognition moiety having a spacer group of a defined length thereon, the recognition moiety capable of binding with tetrameric multivalent neuraminidase.

Abstract translation: 公开了用于检测样品中四聚体多价神经氨酸酶的传感器，其中阳性检测表明样品中存在目标病毒。 还公开了一种三官能组合物，其包括具有与其键合的基团的三官能连接体部分，其包含（a）适于连接到底物的烷基链，（b）能够产生荧光信号的荧光部分，和（c）识别 具有其上限定长度的间隔基的部分，所述识别部分能够与四聚体多价神经氨酸酶结合。

公开(公告)号：US20110154648A1

公开(公告)日：2011-06-30

申请号：US13047593

申请日：2011-03-14

Applicant: Jean Christophe Gabriel ,  George Gruner ,  Philip Collins ,  Basil Swanson ,  Fred Wudl

Inventor： Jean Christophe Gabriel ,  George Gruner ,  Philip Collins ,  Basil Swanson ,  Fred Wudl

IPC: H05K13/00 ,  B82Y99/00

CPC classification number: B82Y30/00 ,  G01N33/5438 ,  G01N33/551 ,  G01N33/566 ,  G01N33/582 ,  Y10T29/49002

Abstract: This invention provides for an apparatus and a method for detecting the presence of pathogenic agents with sensors containing functionalized nanostructures integrated into circuits on silicon chips. The nanostructures are functionalized with molecular transducers that recognize and bind targeted analytes which are diagnostic of the pathogenic agent of interest. The molecular transducer includes a receptor portion, which binds the analyte, and an anchor portion that attaches to the nanostructure. Upon binding of the analyte, a change in molecular configuration represented by the newly formed receptor-analyte complex creates a force that is transmitted to the nanostructure via the anchor portion of the transducer. The effect of the force transmitted to the nanostructure is to alter its conductivity. The change in conductivity of the nanotube thus represents a signal that indicates the presence of the pathogenic agent of interest.

Abstract translation: 本发明提供了一种用于检测病原体的存在的装置和方法，所述传感器包含集成在硅芯片上的电路中的功能化纳米结构。 纳米结构用识别和结合目标分析物的分子换能器进行功能化，这些目标分析物是目标病原体的诊断。 分子换能器包括结合分析物的受体部分和附着到纳米结构的锚定部分。 在分析物结合后，由新形成的受体 - 分析物复合物表示的分子构型的变化产生通过换能器的锚定部分传递到纳米结构的力。 传递到纳米结构的力的作用是改变其导电性。 因此，纳米管的电导率的变化表示表示感兴趣的病原体的存在的信号。