公开(公告)号：US20080071106A1

公开(公告)日：2008-03-20

申请号：US11824588

申请日：2007-06-29

申请人： Bong-Rae Cho

发明人： Bong-Rae Cho

IPC分类号： C07C229/00 ,  C07C211/43

CPC分类号： C07C229/18 ,  C07C309/14 ,  C07F9/106 ,  C09B57/00 ,  G01N33/582

摘要： Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.

摘要翻译： 提供了用于脂筏实时成像的双光子染料。 双光子染料在细胞膜的环境中以高强度发射荧光，并且可以用于清楚地区分细胞膜的疏水和亲水结构域。 因此，使用双光子染料能够令人满意的实际脂筏畴的成像。 此外，由于双光子染料不显示细胞毒性，因此它们可以适用于生物成像应用，例如脂质筏在长时间内的实时成像。

公开(公告)号：US08228266B2

公开(公告)日：2012-07-24

申请号：US11819129

申请日：2007-06-25

申请人： Bong Rae Cho

发明人： Bong Rae Cho

IPC分类号： G09G3/30

CPC分类号： H01L27/3276 ,  H01L27/3272

摘要： An organic electroluminescent device is provided. A gate line and a data line cross each other to define a pixel region, and a power line crosses the gate line and is arranged parallel to the data line. A switching transistor is disposed at a crossing of the gate line and the data line, a driving transistor is disposed at a crossing of the gate line and the power line, and a pixel electrode is formed in the pixel region. Each of the switching transistor and the driving transistor includes a gate electrode formed under a channel layer, and a stopper on the channel layer.

摘要翻译： 提供有机电致发光器件。 栅极线和数据线彼此交叉以限定像素区域，并且电源线跨越栅极线并且平行于数据线布置。 开关晶体管设置在栅极线和数据线的交叉点处，驱动晶体管设置在栅极线和电源线的交叉处，并且像素电极形成在像素区域中。 开关晶体管和驱动晶体管中的每一个包括形成在沟道层下面的栅电极和沟道层上的阻挡层。

公开(公告)号：US08084647B2

公开(公告)日：2011-12-27

申请号：US11997520

申请日：2008-01-21

申请人： Bong-Rae Cho ,  Hwan Myung Kim

发明人： Bong-Rae Cho ,  Hwan Myung Kim

IPC分类号： C07C211/00 ,  A61B5/00 ,  A61B8/00 ,  A61B10/00

CPC分类号： C07C237/04 ,  C07C225/22 ,  G01N33/582

摘要： A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20˜50-fold TPEF enhancement in response to Ca2+, has a dissociation constant (KdTP) of 0.14±0.02 to 0.25±0.03 μM, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 μm in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.

摘要翻译： 提供了用于实时监测细胞内钙离子的双光子探针。 双光子探针非常适用于细胞内钙离子的实时成像，对Ca2 +响应显示20〜50倍TPEF增强，解离常数（KdTP）为0.14±0.02〜0.25±0.03μM，发射 TPEF比现有的单光子荧光Ca2 +探针强5倍。 与以前可用的探针不同，双光子探针可以选择性地检测活细胞和活组织中细胞内游离Ca 2+的动态水平，而不受其他金属离子和膜结合探针的干扰。 此外，双光子探针能够使用双光子显微镜（TPM）在活组织中监测1100-300μm深度的钙波，而不会产生光漂白。 还提供了制备双光子探针的方法和使用双光子探针实时监测细胞内钙离子的方法。

公开(公告)号：US07955815B2

公开(公告)日：2011-06-07

申请号：US12288101

申请日：2008-10-15

申请人： Bong-Rae Cho

发明人： Bong-Rae Cho

IPC分类号： C12Q1/02 ,  G01N21/77 ,  G01N21/76

CPC分类号： G01N33/582 ,  C07C237/04 ,  G01N33/533

摘要： Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by The probes can selectively bind to vesicles in cytosol to emit two-photon excited fluorescence with high intensity. Therefore, the use of the probes enables effective imaging of acidic vesicles. Further provided is a method for imaging acidic vesicles in live cells and tissue using the probes.

摘要翻译： 提供了用于在活细胞和组织中成像酸性囊泡的双光子荧光探针。 探针由探针可以选择性地结合细胞质中的囊泡以发射具有高强度的双光子激发荧光。 因此，使用探针能够有效地成像酸性囊泡。 还提供了使用探针在活细胞和组织中对酸性囊泡进行成像的方法。

公开(公告)号：US08129137B2

公开(公告)日：2012-03-06

申请号：US12454169

申请日：2009-05-12

申请人： Bong-Rae Cho

发明人： Bong-Rae Cho

IPC分类号： C12Q1/02 ,  C07D213/00

CPC分类号： A61K49/0021 ,  C07D213/38

摘要： A two-photon probe for real-time monitoring of intracellular free zinc ions is provided. The two-photon probe is represented by Formula 1: wherein R is H or OCH3. The two-photon probe has high selectivity for Zn2+ and enables very effective and long-term monitoring of intracellular free Zn2+ present in the deep tissue. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular free zinc ions using the two-photon probe.

摘要翻译： 提供了用于实时监测细胞内游离锌离子的双光子探针。 双光子探针由式1表示：其中R是H或OCH 3。 双光子探针对Zn2 +具有很高的选择性，能够对深层组织中存在的细胞内游离Zn2 +进行非常有效和长期的监测。 还提供了制备双光子探针的方法和使用双光子探针实时监测细胞内游离锌离子的方法。

公开(公告)号：US20100105097A1

公开(公告)日：2010-04-29

申请号：US11997520

申请日：2008-01-21

申请人： Bong-Rae Cho

发明人： Bong-Rae Cho

IPC分类号： C12Q1/02 ,  C07C229/00

CPC分类号： C07C237/04 ,  C07C225/22 ,  G01N33/582

摘要： A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20-5 O-fold TPEF enhancement in response to Ca2+, has a dissociation constant (Kdτp) of 0.14±0.02 to 0.25±0.03 μM, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 μm in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe.

摘要翻译： 提供了用于实时监测细胞内钙离子的双光子探针。 双光子探针非常适用于细胞内钙离子的实时成像，对Ca2 +响应显示20-5 O倍TPEF增强，解离常数（Kdτp）为0.14±0.02〜0.25±0.03μM， 比目前可用的单光子荧光Ca2 +探针发射5倍更强的TPEF。 与以前可用的探针不同，双光子探针可以选择性地检测活细胞和活组织中细胞内游离Ca 2+的动态水平，而不受其他金属离子和膜结合探针的干扰。 此外，双光子探针能够使用双光子显微镜（TPM）在活组织中监测1100-300μm深度的钙波，而不会产生光漂白。 还提供了制备双光子探针的方法和使用双光子探针实时监测细胞内钙离子的方法。

公开(公告)号：US08632749B2

公开(公告)日：2014-01-21

申请号：US12876367

申请日：2010-09-07

申请人： Seung Bum Park ,  Bong Rae Cho ,  Hyang Yeon Lee ,  Jong Min Park

发明人： Seung Bum Park ,  Bong Rae Cho ,  Hyang Yeon Lee ,  Jong Min Park

IPC分类号： A61B5/00 ,  A61B8/00 ,  A61B10/00 ,  A61K49/00

CPC分类号： A61B5/00 ,  A61K49/0004 ,  A61K49/0021 ,  A61K49/0052 ,  C07H15/18 ,  C07H15/26 ,  C12Q1/54 ,  G01N33/574 ,  G01N33/58 ,  G01N2500/00

摘要： Disclosed herein are compounds useful as two-photon tracers. Also, methods are provided for visualizing intracellular glucose uptake, screening anticancer agents, and diagnosing cancer using the compounds. They exhibit preferential uptake by cancer cells, penetrability sufficient to allow bright section images, high water solubility, high pH resistance and low toxicity in addition to applicability to living cells in deep tissues over a long period of time.

摘要翻译： 本文公开了可用作双光子示踪剂的化合物。 此外，提供了用于使细胞内葡萄糖摄取可视化，筛选抗癌剂和使用该化合物诊断癌症的方法。 除了适用于长时间深层组织中的活细胞外，它们表现出癌细胞的优先摄取，足以允许亮片图像，高水溶性，高耐pH和低毒性的渗透性。

公开(公告)号：US20130236922A1

公开(公告)日：2013-09-12

申请号：US13476163

申请日：2012-05-21

申请人： Bong-Rae CHO ,  Hoon-Jai CHUN

发明人： Bong-Rae CHO ,  Hoon-Jai CHUN

IPC分类号： G01N21/64 ,  C07C323/25

CPC分类号： G01N21/6486 ,  C07C323/25

摘要： The present disclosure relates to a copper (I) ion-selective fluorescent probe, a method for preparing the same, and a method for diagnosing malignant disease and a diagnosis kit using the probe. The fluorescent probe according to the present disclosure is capable of detecting free copper (I) ions inside cells for a long time with high selectivity and sensitivity for copper (I) ion, with a penetration depth longer than 90 μm in living cells and tissues and without the problems of mistargeting and photobleaching. Accordingly, since a biological sample can be imaged for a long period of time with high resolution without damage, presence of malignance disease in the target biological sample can be diagnosed faster, more accurately and more easily.

摘要翻译： 本公开涉及铜（I）离子选择性荧光探针，其制备方法，以及诊断恶性疾病的方法和使用该探针的诊断试剂盒。 根据本公开的荧光探针能够长时间检测细胞内的游离铜（I）离子，对于铜（I）离子具有高选择性和灵敏度，在活细胞和组织中的渗透深度大于90μm， 没有定位和漂白的问题。 因此，由于可以以高分辨率对生物样品长时间成像而没有损伤，因此可以更快，更准确和更容易地诊断目标生物样品中恶性疾病的存在。

公开(公告)号：US08310612B2

公开(公告)日：2012-11-13

申请号：US11304210

申请日：2005-12-14

申请人： Bong Rae Cho

发明人： Bong Rae Cho

IPC分类号： G02F1/136 ,  G02F1/1333

CPC分类号： G02F1/136227 ,  G02F1/13454 ,  G02F2202/104 ,  H01L27/124 ,  H01L27/1288

摘要： A thin film transistor (TFT) liquid crystal display panel and fabrication method are described. The panel has a data line and a gate line connected with a TFT and formed on the same layer. One of data or gate lines is discontinuous and the other is continuous in a pixel region such that the continuous line bisects the discontinuous line. A passivation film protects the TFT. Contact holes penetrate the passivation film and expose segments of the discontinuous line. A contact electrode connects the segments through the contact holes.

摘要翻译： 描述薄膜晶体管（TFT）液晶显示面板及其制造方法。 面板具有与TFT连接并形成在同一层上的数据线和栅极线。 数据或栅极线之一是不连续的，另一个在像素区域中是连续的，使得连续线将不连续线平分。 钝化膜保护TFT。 接触孔穿透钝化膜并暴露不连续线段。 接触电极通过接触孔连接片段。

公开(公告)号：US20110059022A1

公开(公告)日：2011-03-10

申请号：US12876367

申请日：2010-09-07

申请人： Seung Bum PARK ,  Bong Rae CHO ,  Hyang Yeon LEE ,  Jong Min PARK

发明人： Seung Bum PARK ,  Bong Rae CHO ,  Hyang Yeon LEE ,  Jong Min PARK

IPC分类号： A61K49/00 ,  C07H15/26 ,  C12Q1/02 ,  C12Q1/54 ,  C12Q1/04 ,  C07H1/00

CPC分类号： A61B5/00 ,  A61K49/0004 ,  A61K49/0021 ,  A61K49/0052 ,  C07H15/18 ,  C07H15/26 ,  C12Q1/54 ,  G01N33/574 ,  G01N33/58 ,  G01N2500/00

摘要： Disclosed herein are compounds useful as two-photon tracers. Also, methods are provided for visualizing intracellular glucose uptake, screening anticancer agents, and diagnosing cancer using the compounds. They exhibit preferential uptake by cancer cells, penetrability sufficient to allow bright section images, high water solubility, high pH resistance and low toxicity in addition to applicability to living cells in deep tissues over a long period of time.

摘要翻译： 本文公开了可用作双光子示踪剂的化合物。 此外，提供了用于使细胞内葡萄糖摄取可视化，筛选抗癌剂和使用该化合物诊断癌症的方法。 除了适用于长时间深层组织中的活细胞外，它们表现出癌细胞的优先摄取，足以允许亮片图像，高水溶性，高耐pH和低毒性的渗透性。