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    Enzyme catalyzed isotope labeling
    2.
    发明申请
    Enzyme catalyzed isotope labeling 审中-公开
    酶催化同位素标记

    公开(公告)号：US20050032149A1

    公开(公告)日：2005-02-10

    申请号：US10786457

    申请日：2004-02-26

    申请人： Xudong Yao ,  Catherine Fenselau

    发明人： Xudong Yao ,  Catherine Fenselau

    IPC分类号： C07K1/13 ,  C12P21/06 ,  C12Q1/37 ,  G01N33/58 ,  G01N33/60 ,  G01N33/53

    CPC分类号： C12P21/06 ,  C07K1/13 ,  C12Q1/37 ,  G01N33/585 ,  G01N33/60

    摘要： In a method of enzyme-catalyzed isotope labeling of peptides, peptides are formed from proteins digested by proteolyic enzymes selected from trypsin, chymotrypsin, Lys-C endoprotease, Glu-C endoprotease, an endoprotease that cleaves at the C-terminal side of an amino acid residue on a protein, or a mixture of two or more of these proteolytic enzymes. Thereafter isotopic atoms are incorporated into the peptides in the catalytic presence of proteolyic enzymes selected from trypsin, chymotrypsin, Lys-C endoprotease, Glu-C endoprotease, an endoprotease that cleaves at the C-terminal side of an amino acid residue on a protein, or a mixture of two or more of these proteolytic enzymes.

    摘要翻译： 在肽催化的同位素标记肽的方法中，肽由蛋白酶选择胰蛋白酶，胰凝乳蛋白酶，Lys-C内切蛋白酶，Glu-C内切蛋白酶消化的蛋白质形成，内切蛋白酶在氨基的C-末端侧切割 蛋白质上的酸残基或两种或更多种这些蛋白水解酶的混合物。 此后，在蛋白酶的催化存在下，将同位素原子并入肽中，所述蛋白酶选自胰蛋白酶，胰凝乳蛋白酶，Lys-C内切蛋白酶，Glu-C内切蛋白酶，在蛋白质上的氨基酸残基的C-末端侧切割的内切蛋白酶， 或这些蛋白水解酶中的两种或更多种的混合物。

    Use of matrices comprising liquids and light absorbing particles for analysis of microorganisms by laser desorption mass spectrometry
    3.
    发明授权
    Use of matrices comprising liquids and light absorbing particles for analysis of microorganisms by laser desorption mass spectrometry 失效
    使用包含液体和光吸收颗粒的基质通过激光解吸质谱法分析微生物

    公开(公告)号：US5948610A

    公开(公告)日：1999-09-07

    申请号：US133742

    申请日：1998-08-13

    申请人： Yen-Peng Ho ,  Catherine Fenselau

    发明人： Yen-Peng Ho ,  Catherine Fenselau

    IPC分类号： C12Q1/02 ,  C12Q1/00 ,  C12Q1/04 ,  G01N33/92

    CPC分类号： C12Q1/02 ,  Y10S435/847 ,  Y10S435/848 ,  Y10S435/849 ,  Y10T436/104165 ,  Y10T436/24

    摘要： The present invention is directed to a method of using a liquid matrix containing a black body light absorbing powder to facilitate the analysis of biomarkers from representative microorganisms by laser desorption mass spectrometry. Both an IR laser (1064 nm) and a UV laser (337 nm) were shown to be compatible and both time-of-flight and Fourier-transform mass analyzer were used. In the present implementation gram negative and gram positive bacteria were suspended in a methanol:chloroform solution and added to a cobalt/glycerol matrix, S/N, sensitivity and sampling time are greatly enhanced for polar lipid biomarkers.

    摘要翻译： 本发明涉及使用含有黑体光吸收粉末的液体基质的方法，以便于通过激光解吸质谱法分析来自代表性微生物的生物标志物。 红外激光（1064nm）和紫外激光（337nm）都被证明是兼容的，并且使用飞行时间和傅里叶变换质量分析仪。 在本实施方案中，将革兰氏阴性和革兰氏阳性细菌悬浮于甲醇：氯仿溶液中，并加入到钴/甘油基质中，S / N，极性脂质生物标志物的灵敏度和取样时间均大大增强。

    Viral characterization by direct detection of capsid proteins
    5.
    发明授权
    Viral characterization by direct detection of capsid proteins 失效
    通过直接检测衣壳蛋白进行病毒鉴定

    公开(公告)号：US6037118A

    公开(公告)日：2000-03-14

    申请号：US120861

    申请日：1998-07-22

    申请人： John J. Thomas ,  Catherine Fenselau

    发明人： John J. Thomas ,  Catherine Fenselau

    IPC分类号： G01N33/68 ,  C12Q1/70 ,  G01N1/28 ,  G01N37/00

    CPC分类号： G01N33/6848 ,  G01N33/6851 ,  G01N2333/005

    摘要： The present invention provides a method of direct detecting of capsid proteins from intact viral particles using matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry, which enables viral proteins to be characterized at the femtomolar level in complex biological milieu with minimal or no cleanup.

    摘要翻译： 本发明提供了一种使用基质辅助激光解吸/电离（MALDI）飞行时间质谱法直接检测来自完整病毒颗粒的衣壳蛋白的方法，其使病毒蛋白质能够在复合生物学环境中以飞秒级表征 最少或没有清理。

    Neutralization/chemical reionization tandem mass spectrometry method and apparatus therefor
    7.
    发明授权
    Neutralization/chemical reionization tandem mass spectrometry method and apparatus therefor 失效
    中和/化学反离子串联质谱法及其设备

    公开(公告)号：US5101105A

    公开(公告)日：1992-03-31

    申请号：US608435

    申请日：1990-11-02

    申请人： Catherine Fenselau ,  Robert J. Cotter

    发明人： Catherine Fenselau ,  Robert J. Cotter

    IPC分类号： H01J49/14

    CPC分类号： H01J49/145 ,  H01J49/14

    摘要： A mass spectrometer system including a device for supplying a protonated ion species; a single chamber having an entrance and an exit slit, the chamber receiving the supplied protonated ion and transmitting a reprotonated ion through the exit slit; a device for supplying a predetermined reagent gas within the chamber; a device for supplying an electron beam in said chamber such that the electron beam reacts with the supplied predetermined reagent gas to provide neutral reagent gas and protonated reagent gas within the chamber, the supplied ion species reacting with the neutral reagent gas to neutralize the ion species, thereby providing neutral species within the chamber, the neutral species reacting with the protonated reagent gas to provide reionized species within the chamber; and a device, coupled to the exit slit of the chamber, for extracting the reionized species.

    摘要翻译： 一种质谱仪系统，包括用于提供质子化离子物质的装置; 具有入口和出口狭缝的单个室，所述室接收所提供的质子离子并将再现离子透过出口狭缝; 用于在所述室内供应预定的反应气体的装置; 用于在所述室中供应电子束的装置，使得电子束与所提供的预定反应气体反应以在室内提供中性反应气体和质子化反应气体，所供应的离子物质与中性反应气体反应以中和离子种类 从而在室内提供中性物质，中性物质与质子化的反应气体反应以在室内提供去离子物质; 以及耦合到所述室的出口狭缝的用于提取所述再离子化物质的装置。