公开(公告)号：US06608040B1

公开(公告)日：2003-08-19

申请号：US09297808

申请日：2001-09-27

申请人： Ching-Kuan Lin ,  Thomas Chau-Jen Lee ,  Hsing-Wen Sung

发明人： Ching-Kuan Lin ,  Thomas Chau-Jen Lee ,  Hsing-Wen Sung

IPC分类号： A01N4304

CPC分类号： A61L27/507 ,  A61F2310/00365 ,  A61F2310/00383 ,  A61L15/28 ,  A61L15/32 ,  A61L15/325 ,  A61L24/10 ,  A61L24/102 ,  A61L24/104 ,  A61L26/0023 ,  A61L26/0033 ,  A61L26/0038 ,  A61L27/20 ,  A61L27/22 ,  A61L27/222 ,  A61L27/24 ,  A61L27/3616 ,  A61L27/3645 ,  A61L27/3662 ,  A61L27/3687 ,  A61L2430/40 ,  C08L5/08

摘要： Biocompatible cross-linked materials, suitable for use in implants, wound dressings, and blood substitutes, are described. The materials are prepared by cross-linking biological substances, such as collagen, chitosan, or hemoglobin, with genipin, a naturally occurring cross-linking agent. The cross-linking agent has much lower toxicity than conventionally used reagents, and the cross-linked products have good thermal and mechanical stability as well as biocompatibility.

摘要翻译： 描述适用于植入物，伤口敷料和血液代用品的生物相容性交联材料。 这些材料是通过将天然存在的交联剂与天蚕素交联生物物质如胶原蛋白，壳聚糖或血红蛋白来制备的。 交联剂的毒性低于常规使用的试剂，交联产物具有良好的热和机械稳定性以及生物相容性。

公开(公告)号：US5407579A

公开(公告)日：1995-04-18

申请号：US86712

申请日：1993-07-02

申请人： Chau-Jen Lee ,  Pei Kan

发明人： Chau-Jen Lee ,  Pei Kan

IPC分类号： B01D21/26 ,  C07K14/805 ,  B01D11/00 ,  A61K35/14 ,  B01D61/00

CPC分类号： C07K14/805 ,  B01D21/262 ,  B01D2221/10 ,  Y10T436/25375 ,  Y10T436/255

摘要： Disclosed is an improved method for hemoglobin purification utilizing a novel two-step dual-aqueous-phase extraction technique to separate hemoglobin from red cell membrane stroma and other protein contaminants. In the first step, a first dual-aqueous-phase liquid system is prepared which comprises an upper aqueous phase containing polyethylene glycol in water and a lower aqueous phase containing a phosphate buffer at a pH of about 10. In the second step, a second dual-aqueous-phase liquid system is prepared which comprises an upper aqueous phase, which contains the polyethylene glycol phase containing the hemoglobin extracted in the first step, and a lower aqueous phase containing a new phosphate buffer at a pH of less than 7.5. After the second extraction step, purified hemoglobin solution can then be obtained by removing the phosphate salt and the minute amounts of polyethylene glycol contained in the lower phase.

摘要翻译： 公开了一种利用新型两步双水相萃取技术将血红蛋白与红细胞膜基质和其他蛋白质污染物分离的血红蛋白纯化的改进方法。 在第一步骤中，制备第一个双水相液体系统，其包含在水中含有聚乙二醇的上部水相和含有pH约为10的磷酸盐缓冲液的下部水相。在第二步中， 制备双水相液体系统，其包含含有在第一步骤中提取的含有血红蛋白的聚乙二醇相的上部水相和含有pH小于7.5的新的磷酸盐缓冲液的下部水相。 在第二次提取步骤之后，可以通过除去磷酸盐和少量的下一相中含有的聚乙二醇来获得纯化的血红蛋白溶液。