公开(公告)号：US20050255090A1

公开(公告)日：2005-11-17

申请号：US11186559

申请日：2005-07-21

Applicant: Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Song

Inventor： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Song

IPC: G01N33/569 ,  C12N1/20 ,  C12N9/88 ,  C12P13/22 ,  C12Q1/06 ,  C12R1/01 ,  G01N21/77 ,  A61K45/00

CPC classification number: C12R1/01 ,  C12N9/88 ,  C12P13/227

Abstract: The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

Abstract translation: 本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i））i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法，使用硝酸盐呼吸显示低生长产量的共生杆菌SC-1及其相对共生菌的生长方法，以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此，在本发明中由共生杆菌（Toibii）SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂，其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸（L-DOPA）和L-色氨酸。