LIGHT GUIDE PLATE STAMP AND METHOD OF MANUFACTURING THE SAME
    3.
    发明申请
    LIGHT GUIDE PLATE STAMP AND METHOD OF MANUFACTURING THE SAME 有权
    光导板印章及其制造方法

    公开(公告)号:US20120006090A1

    公开(公告)日:2012-01-12

    申请号:US13240982

    申请日:2011-09-22

    IPC分类号: B21D37/01

    CPC分类号: B21D47/04

    摘要: A stamp includes a metal supporting layer, a pattern forming layer and an adhesive layer. The metal supporting layer has a first thermal conductivity. The pattern forming layer is disposed on the metal supporting layer and has a surface with a molding pattern formed thereon. The adhesive layer is disposed between the metal supporting layer and the pattern forming layer to couple the pattern forming layer to the metal supporting layer, and has a second thermal conductivity lower than the first thermal conductivity. Thus, strength of the stamp may be improved, and deformation of the stamp during the process of manufacturing a light guide plate may be reduced or prevented.

    摘要翻译: 印模包括金属支撑层,图案形成层和粘合剂层。 金属支撑层具有第一导热性。 图案形成层设置在金属支撑层上,并具有在其上形成有模制图案的表面。 粘合剂层设置在金属支撑层和图案形成层之间,以将图案形成层耦合到金属支撑层,并且具有比第一热导率低的第二热导率。 因此,可以提高印模的强度,并且可以减少或防止在制造导光板的过程中印模的变形。

    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS
    4.
    发明申请
    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS 审中-公开
    PENTOSE磷酸盐路径升级以增加转基因微生物中非生物利益产品的生产

    公开(公告)号:US20110244512A1

    公开(公告)日:2011-10-06

    申请号:US13074069

    申请日:2011-03-29

    摘要: Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase [“G6PDH”] and 6-phospho-gluconolactonase [“6PGL”] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid [“PUFA”] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate [“NADPH”], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

    摘要翻译: 协调调节编码葡萄糖6-磷酸脱氢酶[“G6PDH”]和6-磷酸 - 葡糖酸半乳糖醛酸酶[“6PGL”]的基因在含油酵母,解脂耶氏酵母的转基因菌株中的过表达,其包含功能性多不饱和脂肪酸[“PUFA “]生物合成途径导致了PUFA的生产增加,并增加了耶氏酵母细胞中的总脂质含量。 这是通过增加转基因微生物中烟酰胺腺嘌呤二核苷酸磷酸盐还原形式的细胞可利用度[“NADPH”],这是还原性生物合成反应的重要还原等价物。

    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA
    6.
    发明申请
    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA 有权
    来自改良的YARROWIA LIPOLYTICA优化菌株的高选择性酸性油

    公开(公告)号:US20100317735A1

    公开(公告)日:2010-12-16

    申请号:US12814880

    申请日:2010-06-14

    摘要: Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译: 描述了能够产生包含大于50重量%的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株,以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“% TFAs“],并且具有至少3.1%的EPA%TFA与比率为TFA的亚油酸的比率。 除了其他异源&Dgr。9延伸酶,Dgr。8去饱和酶,Dgr。5去饱和酶,Dgr 17脱饱和酶,Dgr。12去饱和酶,C16外,这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶,丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法,从细胞获得的油及其产物。

    MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS
    7.
    发明申请
    MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS 有权
    用于改变OLE内含物的SNF1激酶的操作

    公开(公告)号:US20100062502A1

    公开(公告)日:2010-03-11

    申请号:US12549439

    申请日:2009-08-28

    摘要: Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

    摘要翻译: 公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量,多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法 。 优选地,编码SNF1蛋白激酶的Snf1α-亚基,Gal83&bgr-亚基或Snf4γ-亚基的染色体基因,编码Sak1,Hxk2,Glk1或Reg1的上游调节基因,或编码Rme1,Cbr1或 与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比,Snf3在含油酵母解脂耶氏酵母解脂耶氏酵母的PUFA产生菌株中被操纵,导致总脂质含量增加。

    SURFACE EXPRESSION METHOD OF PEPTIDES P5 AND ANAL3 USING THE GENE ENCODING POLY-GAMMA-GLUTAMATE SYNTHETASE
    9.
    发明申请
    SURFACE EXPRESSION METHOD OF PEPTIDES P5 AND ANAL3 USING THE GENE ENCODING POLY-GAMMA-GLUTAMATE SYNTHETASE 有权
    使用基因编码多糖 - 谷氨酸合成酶的肽P5和ANAL3的表面表达方法

    公开(公告)号:US20070253935A1

    公开(公告)日:2007-11-01

    申请号:US11622158

    申请日:2007-01-11

    IPC分类号: A61K39/00 C12N1/21 C12N15/00

    CPC分类号: C07K14/32

    摘要: The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.

    摘要翻译: 本发明涉及使用载体表达在微生物表面上表现出具有两亲性的肽抗生素P 5 3和Anal 3 35的各种抗细菌,抗真菌和抗癌活性61,63,65,67,69,71的方法, 含有源自芽孢杆菌属的外膜蛋白基因(pgsBCA)。 菌株并参与了聚-γ-谷氨酸的合成。 此外,本发明涉及具有表面表达的肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明,肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此,本发明的方法具有非常高的工业实用性。 此外,除了P 5 3和Anal 3 35之外,本发明可以应用于其他肽抗生素。