公开(公告)号：US5849491A

公开(公告)日：1998-12-15

申请号：US716942

申请日：1996-09-20

Applicant: Christopher C. A. Radomski ,  Kah Tong Seow ,  R. Antony J. Warren ,  Wai Ho Yap

Inventor： Christopher C. A. Radomski ,  Kah Tong Seow ,  R. Antony J. Warren ,  Wai Ho Yap

IPC: C12N15/09 ,  C12N9/24 ,  C12N15/10 ,  C12N15/56 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6876 ,  C12N15/10 ,  C12N9/248 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y302/01008 ,  C12Y302/01032

Abstract: Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.

Abstract translation: 通过使用简并引物的PCR扩增从土壤中回收木聚糖酶DNA。 由于土壤样品的复杂性，回收的产品很可能包含多于一种的多核苷酸。 在通过测序表征之前，将这些回收的拷贝克隆到宿主生物体中以产生每个单独物种的额外拷贝。 发现与已知的木聚糖酶不同的回收的DNA可以以多种方式用于促进用于工业应用的新型木聚糖酶的生产。 首先，回收的DNA或对应于其部分的探针可用作探针以筛选DNA文库并回收完整的木聚糖酶基因，包括回收的DNA的独特区域。 其次，将回收的DNA或与其部分相当的多核苷酸插入已知的木聚糖酶基因中以产生具有回收DNA序列变异的重组木聚糖酶基因。

公开(公告)号：US06441148B1

公开(公告)日：2002-08-27

申请号：US09130337

申请日：1998-08-06

Applicant: Christopher C. A. Radomski ,  Kah Tong Seow ,  R. Antony J. Warren ,  Wai Ho Yap

Inventor： Christopher C. A. Radomski ,  Kah Tong Seow ,  R. Antony J. Warren ,  Wai Ho Yap

IPC: C07H2102

CPC classification number: C12Q1/6876 ,  C12N9/248 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y302/01008 ,  C12Y302/01032

Abstract: Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.

Abstract translation: 通过使用简并引物的PCR扩增从土壤中回收木聚糖酶DNA。 由于土壤样品的复杂性，回收的产品很可能包含多于一种的多核苷酸。 在通过测序表征之前，将这些回收的拷贝克隆到宿主生物体中以产生每个单独物种的额外拷贝。 发现与已知的木聚糖酶不同的回收的DNA可以以多种方式用于促进用于工业应用的新型木聚糖酶的生产。 首先，回收的DNA或对应于其部分的探针可用作探针以筛选DNA文库并回收完整的木聚糖酶基因，包括回收的DNA的独特区域。 其次，将回收的DNA或与其部分相当的多核苷酸插入已知的木聚糖酶基因中以产生具有回收DNA序列变异的重组木聚糖酶基因。