公开(公告)号：US20070093971A1

公开(公告)日：2007-04-26

申请号：US11435495

申请日：2006-05-16

申请人： James Candy ,  David Clague ,  Christopher Lee ,  Robert Rudd ,  Alan Burnham ,  Joseph Tringe

发明人： James Candy ,  David Clague ,  Christopher Lee ,  Robert Rudd ,  Alan Burnham ,  Joseph Tringe

IPC分类号： G01L5/00

CPC分类号： G01N29/036 ,  G01N2291/0256

摘要： A method of using physics-based signal processing algorithms for micromachined cantilever arrays. The methods utilize deflection of a micromachined cantilever that represents the chemical, biological, or physical element being detected. One embodiment of the method comprises the steps of modeling the deflection of the micromachined cantilever producing a deflection model, sensing the deflection of the micromachined cantilever and producing a signal representing the deflection, and comparing the signal representing the deflection with the deflection model.

摘要翻译： 一种使用基于物理学的信号处理算法进行微机械悬臂阵列的方法。 该方法利用表示所检测的化学，生物或物理元件的微机械悬臂的偏转。 该方法的一个实施例包括以下步骤：对产生偏转模型的微机械悬臂的偏转进行建模，感测微机械悬臂的偏转并产生表示偏转的信号，并将表示偏转的信号与偏转模型进行比较。

公开(公告)号：US20050272042A1

公开(公告)日：2005-12-08

申请号：US10727779

申请日：2003-12-03

申请人： Shea Gardner ,  Raymond Mariella ,  Allen Christian ,  Jennifer Young ,  David Clague

发明人： Shea Gardner ,  Raymond Mariella ,  Allen Christian ,  Jennifer Young ,  David Clague

IPC分类号： C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6844 ,  C07H21/00 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1031 ,  C12Q1/6813 ,  C12Q1/686 ,  C12Q2521/101 ,  C12Q2533/107 ,  C12Q2525/143

摘要： A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.

摘要翻译： 制备用户定义序列的DNA分子的方法。 该方法包括以下步骤：预先选择将包含用户定义序列的DNA分子的多个DNA序列片段，以及将多个DNA序列片段与至少一种聚合酶结合， DNA序列片段连接以产生用户定义序列的DNA分子。 序列段可以是长度为n，其中n是偶或奇整数。 在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。 在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。 在一个实施方案中，起始序列片段具有不同长度，n，n + 1，n + 2等

公开(公告)号：US20060223124A1

公开(公告)日：2006-10-05

申请号：US11447754

申请日：2006-06-05

申请人： David Clague ,  Elizabeth Wheeler ,  Abraham Lee

发明人： David Clague ,  Elizabeth Wheeler ,  Abraham Lee

IPC分类号： G01N33/567 ,  G01N33/53

CPC分类号： G01N15/1404 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/142 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/117497

摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.

公开(公告)号：US20050272096A1

公开(公告)日：2005-12-08

申请号：US10862998

申请日：2004-06-07

申请人： David Clague ,  Elizabeth Wheeler ,  Abraham Lee

发明人： David Clague ,  Elizabeth Wheeler ,  Abraham Lee

IPC分类号： C12M1/34 ,  G01N15/00 ,  G01N15/14 ,  G01N33/53 ,  G01N33/542 ,  G01N33/567

CPC分类号： G01N15/1404 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/142 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/117497

摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.

摘要翻译： 流式细胞仪包括用于检测样品的流动池，流动池中的油相，流动池中的水相，油相和水相之间的油 - 水界面，用于在该油相和水相之间检测样品的检测器 油 - 水界面和可操作地连接到样品的疏水单元。 疏水单元附着在样品上。 将样品和疏水单元置于油水组合中。 在油相和水相之间的界面处检测样品。