Method for identifying modulators of sulfotransferase activity
    2.
    发明授权
    Method for identifying modulators of sulfotransferase activity 有权
    识别磺基转​​移酶活性调节剂的方法

    公开(公告)号:US06713274B2

    公开(公告)日:2004-03-30

    申请号:US10145648

    申请日:2002-05-13

    IPC分类号: C12G148

    CPC分类号: C12Q1/48 Y10S435/968

    摘要: The instant invention provides methods for identifying agents that modulate an enzymatic activity of a carbohydrate sulfotransferase. The methods generally involve contacting the sulfotransferase, in a reaction solution, with a sulfate donor, a test agent, and a polymeric sulfate acceptor that is readily separated from the reaction solution. Determination of an effect of the test agent on the sulfotransferase is by detecting the amount of sulfate in the polymeric sulfate acceptor that has been separated from the reaction solution. The invention further provides kits for use in carrying out the subject methods.

    摘要翻译: 本发明提供了鉴定调节碳水化合物转移酶的酶活性的试剂的方法。 所述方法通常包括使反应溶液中的磺基转移酶与硫酸盐供体,试剂和聚合硫酸酯受体接触,其易于与反应溶液分离。 测定试剂对磺基转移酶的影响的测定是通过检测从反应溶液中分离的聚合硫酸酯受体中的硫酸盐的量。 本发明还提供了用于实施本发明方法的试剂盒。