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    Aldehyde tags, uses thereof in site-specific protein modification
    1.
    发明授权
    Aldehyde tags, uses thereof in site-specific protein modification 有权
    醛标签，其在位点特异性蛋白质修饰中的用途

    公开(公告)号：US08729232B2

    公开(公告)日：2014-05-20

    申请号：US12921131

    申请日：2009-03-20

    申请人： Jason S. Rush ,  Carolyn Bertozzi

    发明人： Jason S. Rush ,  Carolyn Bertozzi

    IPC分类号： C07K1/00 ,  C07K16/00 ,  C12N11/00

    CPC分类号： C12P21/02 ,  C07K2319/20

    摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.

    摘要翻译： 本发明的特征在于通过掺入醛标记蛋白的位点特异性修饰的组合物和方法。 通过甲酰甘氨酸生成酶（FGE）的作用，在醛标记的硫酸酯酶基序上进行酶促修饰产生甲酰甘氨酸（FGly）残基。 FGly残基的醛部分可以用作化学处理，用于将目标部分的位点特异性连接到多肽。

    Chemoselective ligation
    4.
    发明申请
    Chemoselective ligation 有权
    化学选择性连接

    公开(公告)号：US20050148032A1

    公开(公告)日：2005-07-07

    申请号：US11039478

    申请日：2005-01-19

    申请人： Eliana Saxon ,  Carolyn Bertozzi

    发明人： Eliana Saxon ,  Carolyn Bertozzi

    IPC分类号： C07F9/50 ,  C07F9/6506 ,  C07F9/655 ,  C07F9/6561 ,  C07K1/02 ,  C07K1/04 ,  C07K1/107 ,  C12N5/02 ,  G01N33/53 ,  G01N33/567 ,  C12Q1/68 ,  C07F9/22 ,  C07C247/16

    CPC分类号： C07K1/1077 ,  C07F9/5022 ,  C07F9/6506 ,  C07F9/65522 ,  C07F9/6561 ,  C07H7/02 ,  C07H13/00 ,  C07H15/203 ,  C07H15/26 ,  C07K1/023 ,  C07K1/04 ,  C07K1/1075

    摘要： The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

    摘要翻译： 本发明的特征在于可以在生理条件下进行的化学选择性连接反应。 通常，本发明涉及特异性改造的膦的缩合，其可以提供在两个反应性配对物之间形成酰胺键，产生包含膦部分的最终产物，或者可被工程化以包含可切割连接体，使得 将膦的取代基转移到叠氮化物中，释放氧化的膦副产物并在最终产物中产生天然酰胺键。 反应的选择性及其与水性环境的相容性在体内（例如，在细胞表面或细胞内）和体外（例如，肽和其他聚合物的合成，修饰（例如，标记的）氨基酸的产生 ）。

    Chemoselective ligation
    9.
    发明授权
    Chemoselective ligation 有权

    公开(公告)号：US07122703B2

    公开(公告)日：2006-10-17

    申请号：US10384099

    申请日：2003-03-06

    申请人： Eliana Saxon ,  Carolyn Bertozzi

    发明人： Eliana Saxon ,  Carolyn Bertozzi

    IPC分类号： C12N5/02 ,  C12P7/26 ,  C07F5/00 ,  C07F9/02

    CPC分类号： C07K1/1077 ,  C07F9/5022 ,  C07F9/6506 ,  C07F9/65522 ,  C07F9/6561 ,  C07H7/02 ,  C07H13/00 ,  C07H15/203 ,  C07H15/26 ,  C07K1/023 ,  C07K1/04 ,  C07K1/1075

    摘要： The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

    Biomimetic hydrogel materials
    10.
    发明授权
    Biomimetic hydrogel materials 失效
    仿生水凝胶材料

    公开(公告)号：US06552103B1

    公开(公告)日：2003-04-22

    申请号：US09574938

    申请日：2000-05-19

    申请人： Carolyn Bertozzi ,  Ravindranath Mukkamala ,  Oing Chen ,  Hopin Hu ,  Dominique Baude

    发明人： Carolyn Bertozzi ,  Ravindranath Mukkamala ,  Oing Chen ,  Hopin Hu ,  Dominique Baude

    IPC分类号： G02C704

    CPC分类号： G02B1/043 ,  A61L27/16 ,  A61L27/52 ,  A61L2430/16 ,  C08F220/38 ,  C08F220/54 ,  Y10S524/916 ,  C08L51/02 ,  C08L33/14

    摘要： Novel biomimetic hydrogel materials and methods for their preparation. Hydrogels containing acrylamide-functionalized carbohydrate, sulfoxide, sulfide or sulfone copolymerized with a hydrophilic or hydrophobic copolymerizing material selected from the group consisting of an acrylamide, methacrylamide, acrylate, methacrylate, vinyl and a derivative thereof present in concentration from about 1 to about 99 wt %. and methods for their preparation. The method of use of the new hydrogels for fabrication of soft contact lenses and biomedical implants.

    摘要翻译： 新型仿生水凝胶材料及其制备方法。 含有丙烯酰胺官能化碳水化合物，亚砜，硫化物或砜的水凝胶与选自丙烯酰胺，甲基丙烯酰胺，丙烯酸酯，甲基丙烯酸酯，乙烯基及其衍生物的亲水或疏水共聚材料共聚合，其浓度为约1至约99重量％ ％。 及其制备方法。 使用新型水凝胶制造软性隐形眼镜和生物医学植入物的方法。