公开(公告)号：US08921098B2

公开(公告)日：2014-12-30

申请号：US11406148

申请日：2006-04-18

申请人： Michael R. Gambini ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Eugene F. Young ,  Susan Atwood Stone ,  Judith K. Atwood

发明人： Michael R. Gambini ,  John G. Atwood ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Eugene F. Young

IPC分类号： C12M1/34 ,  C12M3/00 ,  G01N21/27 ,  B01L7/00 ,  B01L3/00 ,  G01N21/64

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An instrument for monitoring replication of DNA is provided.

摘要翻译： 提供了一种用于监测DNA复制的工具。

公开(公告)号：US07183103B2

公开(公告)日：2007-02-27

申请号：US10370846

申请日：2003-02-20

申请人： Michael R. Gambini ,  Susan Atwood Stone, legal representative ,  Judith K. Atwood, legal representative ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

发明人： John G. Atwood, deceased

IPC分类号： C12M1/34

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US06818437B1

公开(公告)日：2004-11-16

申请号：US09700536

申请日：2001-11-29

申请人： Michael R. Gambini ,  John G. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

发明人： Michael R. Gambini ,  John G. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

IPC分类号： C12M134

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

摘要翻译： 光学仪器监测具有温度循环阻滞的反应装置中DNA的PCR复制，所述反应装置具有小瓶反应成分，包括在双链DNA存在下发荧光的染料。 分束器将激发光束传递到小瓶以使染料发荧光。 来自染料的发射光束通过分束器传递到CCD检测器，处理器从该CCD检测器计算DNA浓度。 具有多个参考发射器的参考条发射不同强度的参考光束，处理器从该参考光束选择用于补偿漂移的最佳发射器。 自动调整曝光时间以保持CCD和处理器的最佳动态范围。 分束器和相关的滤光器的模块与选定的染料相关联，并且可替代不同的染料。

公开(公告)号：US07008789B2

公开(公告)日：2006-03-07

申请号：US10216620

申请日：2002-08-09

申请人： Michael R. Gambini ,  Susan Atwood Stone, legal representative ,  Judith K. Atwood, legal representative ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

发明人： John G. Atwood, deceased

IPC分类号： C12M1/34

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US08557566B2

公开(公告)日：2013-10-15

申请号：US11333483

申请日：2006-01-17

申请人： Michael R. Gambini ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Susan Atwood Stone ,  Judith K. Atwood

发明人： Michael R. Gambini ,  John G. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US4419575A

公开(公告)日：1983-12-06

申请号：US269305

申请日：1981-06-02

申请人： Edward J. Lakatos ,  Paul C. Talmadge

发明人： Edward J. Lakatos ,  Paul C. Talmadge

IPC分类号： G01J3/28 ,  G01J3/12 ,  G01N21/01 ,  G01J3/00

CPC分类号： G01J3/12

摘要： An apparatus useful for positioning a light filter includes an incrementally stepped motor having the light filter affixed thereto. The apparatus also includes means for detecting a light signal and incrementing the motor in response thereto.

摘要翻译： 用于定位光过滤器的装置包括具有附着于其上的光过滤器的增量阶梯式电动机。 该装置还包括用于检测光信号并响应于此使电机递增的装置。

公开(公告)号：US09671342B2

公开(公告)日：2017-06-06

申请号：US11804151

申请日：2007-05-17

申请人： Michael R. Gambini ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Eugene F. Young ,  Susan Atwood Stone

发明人： Michael R. Gambini ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Eugene F. Young ,  Susan Atwood Stone

IPC分类号： C12M1/34 ,  C12M3/00 ,  G01N21/64 ,  C12Q1/68 ,  B01L7/00 ,  G01N21/27 ,  B01L3/00

CPC分类号： G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/0303 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

摘要： An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.