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1.发明授权Plasmid vector, method for detecting gene promoter activity, and assay kit 有权质粒载体，检测基因启动子活性的方法和测定试剂盒公开(公告)号：US09040294B2
公开(公告)日：2015-05-26
申请号：US13593912
申请日：2012-08-24
申请人： Eiichi Akahoshi , Mitsuko Ishihara
发明人： Eiichi Akahoshi , Mitsuko Ishihara
CPC分类号： C12N15/63 , C12N15/69 , C12N15/85 , C12Q1/66 , C12Q1/6897
摘要： According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.
摘要翻译： 根据一个实施方案，第一个基因编码报道蛋白。 第一个基因位于基因启动子的下游。 第二个基因位于基因启动子的下游并编码复制起始结合蛋白。 在第一基因和第二基因之间设置内部核糖体进入位点。 转录终止信号序列编码终止第一基因和第二基因的转录的信号。 复制起始序列由复制起始结合蛋白识别。
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2.发明申请公开(公告)号：US20090061474A1
公开(公告)日：2009-03-05
申请号：US12143202
申请日：2008-06-20
IPC分类号： C12Q1/02
CPC分类号： G01N33/5058 , G01N33/5014 , G01N2333/4703
摘要： The invention provides neuron-derived cells obtained by transfecting a receptor-expressing nucleic acid having an aryl hydrocarbon receptor gene, wherein outgrowth of neurites is not observed without adding a substance for the aryl hydrocarbon receptor, and outgrowth of neurites is observed by adding the substance for the aryl hydrocarbon receptor. The invention also provides a method for determining the presence of neurotoxicity of a test substance, a method for acquiring a marker for determining the presence of neurotoxicity of the test substance, a method for acquiring a marker for neurological dysfunction, and a method for determining the effect of the test substance on neurological dysfunction using such cells.
摘要翻译： 本发明提供了通过转染具有芳基烃受体基因的受体表达核酸获得的神经元衍生细胞，其中不添加神经突的物质而不添加芳基烃受体的物质，并且通过添加物质观察到神经突的生长 用于芳基烃受体。 本发明还提供了一种测定被检物质的神经毒性的存在的方法，获得测定物质的神经毒性的存在的标记物的获取方法，获取神经功能障碍的标记物的方法， 使用这种细胞的测试物质对神经功能障碍的作用。
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3.发明授权公开(公告)号：US07413898B2
公开(公告)日：2008-08-19
申请号：US11442314
申请日：2006-05-30
IPC分类号： C12N5/00 , C12N5/08 , C12N5/10 , G01N38/574 , C07H21/02
CPC分类号： G01N33/5058 , G01N33/5014 , G01N2333/4703
摘要： The invention provides neuron-derived cells obtained by transfecting a receptor-expressing nucleic acid having an aryl hydrocarbon receptor gene, wherein outgrowth of neurites is not observed without adding a substance for the aryl hydrocarbon receptor, and outgrowth of neurites is observed by adding the substance for the aryl hydrocarbon receptor. The invention also provides a method for determining the presence of neurotoxicity of a test substance, a method for acquiring a marker for determining the presence of neurotoxicity of the test substance, a method for acquiring a marker for neurological dysfunction, and a method for determining the effect of the test substance on neurological dysfunction using such cells.
摘要翻译： 本发明提供了通过转染具有芳基烃受体基因的受体表达核酸获得的神经元衍生细胞，其中不添加神经突的物质而不添加芳基烃受体的物质，并且通过添加物质观察到神经突的生长 用于芳基烃受体。 本发明还提供了一种测定被检物质的神经毒性的存在的方法，获得测定物质的神经毒性的存在的标记物的获取方法，获取神经功能障碍的标记物的方法， 使用这种细胞的测试物质对神经功能障碍的作用。
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4.发明申请公开(公告)号：US20060275275A1
公开(公告)日：2006-12-07
申请号：US11442314
申请日：2006-05-30
CPC分类号： G01N33/5058 , G01N33/5014 , G01N2333/4703
摘要： The invention provides neuron-derived cells obtained by transfecting a receptor-expressing nucleic acid having an aryl hydrocarbon receptor gene, wherein outgrowth of neurites is not observed without adding a substance for the aryl hydrocarbon receptor, and outgrowth of neurites is observed by adding the substance for the aryl hydrocarbon receptor. The invention also provides a method for determining the presence of neurotoxicity of a test substance, a method for acquiring a marker for determining the presence of neurotoxicity of the test substance, a method for acquiring a marker for neurological dysfunction, and a method for determining the effect of the test substance on neurological dysfunction using such cells.
摘要翻译： 本发明提供了通过转染具有芳基烃受体基因的受体表达核酸获得的神经元衍生细胞，其中不添加神经突的物质而不添加芳基烃受体的物质，并且通过添加物质观察到神经突的生长 用于芳基烃受体。 本发明还提供了一种测定被检物质的神经毒性的存在的方法，获得测定物质的神经毒性的存在的标记物的获取方法，获取神经功能障碍的标记物的方法， 使用这种细胞的测试物质对神经功能障碍的作用。
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5.发明申请GENETICALLY MODIFIED ANIMAL FOR USE IN EVALUATING HARMFULNESS OF TEST SUBSTANCE 有权用于评估测试物质有害性的遗传修饰动物公开(公告)号：US20100132057A1
公开(公告)日：2010-05-27
申请号：US12559303
申请日：2009-09-14
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/105 , A01K2267/0393 , C07K14/4702 , C12N9/0073 , C12N15/85 , C12N15/8509 , C12N2830/008 , C12N2830/15 , C12N2830/30 , C12N2830/85 , G01N21/763 , G01N33/5088
摘要： A genetically modified animal that includes an introduced DNA including a functionally linked aryl hydrocarbon receptor-binding enhancer located 5′-upstream of a tyrosine hydroxylase gene, promoter of any type, reporter gene, and poly(A) addition signal.
摘要翻译： 一种遗传修饰动物，其包括引入的DNA，其包括位于酪氨酸羟化酶基因5'上游的任何功能性连接的芳基烃受体结合增强子，任何类型的启动子，报告基因和聚（A）加成信号。
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6.发明授权Reporter vector presenting extracellular binding capacity to metallic compounds 有权报道载体呈现金属化合物的细胞外结合能力公开(公告)号：US09442104B2
公开(公告)日：2016-09-13
申请号：US13429926
申请日：2012-03-26
申请人： Eiichi Akahoshi , Mitsuko Ishihara
发明人： Eiichi Akahoshi , Mitsuko Ishihara
IPC分类号： A61K49/16 , C07K16/00 , G01N33/50 , A61K49/18 , G01N33/84 , C12N15/85 , C07K16/44 , A61K48/00
CPC分类号： G01N33/5005 , A61K48/005 , A61K49/16 , A61K49/1896 , C07K16/44 , C07K2317/622 , C07K2319/02 , C12N15/85 , G01N33/84
摘要： According to one embodiment, a reporter vector presenting an extracellular binding capacity to metallic compounds contains a nucleotide sequence exhibiting a promoter activity depending on a specific condition, a nucleotide sequence encoding a metallic compound-binding peptide presented extracellularly, and a nucleotide sequence encoding transcription termination signals.
摘要翻译： 根据一个实施方案，向金属化合物呈递细胞外结合能力的报道载体包含根据特定条件显示启动子活性的核苷酸序列，编码胞外呈递的金属化合物结合肽的核苷酸序列和编码转录终止的核苷酸序列 信号。
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7.发明授权公开(公告)号：US08685645B2
公开(公告)日：2014-04-01
申请号：US12886642
申请日：2010-09-21
申请人： Eiichi Akahoshi , Mitsuko Ishihara
发明人： Eiichi Akahoshi , Mitsuko Ishihara
CPC分类号： C12N15/1051 , C40B40/08 , C40B50/06
摘要： According to one embodiment, a method of screening an enhancer and/or a promoter, includes culturing a host cell into which an amplifiable vector is introduced, extracting the vector from the host cell and obtaining the DNA fragment from the extracted vector, wherein the vector includes a DNA fragment to be determined, a gene that is functionally linked downstream of the DNA fragment and encodes a protein to initiate self-replication, and a gene that encodes a replication origin sequence.
摘要翻译： 根据一个实施方案，筛选增强子和/或启动子的方法包括培养其中引入可扩增载体的宿主细胞，从宿主细胞中提取载体并从提取的载体中获得DNA片段，其中载体 包括要测定的DNA片段，功能上连接在DNA片段下游并编码蛋白质以启动自身复制的基因以及编码复制起始序列的基因。
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8.发明申请REPORTER VECTOR PRESENTING EXTRACELLULAR BINDING CAPACITY TO METALLIC COMPOUNDS 有权记录向量向金属化合物提供细胞结合能力公开(公告)号：US20120295274A1
公开(公告)日：2012-11-22
申请号：US13429926
申请日：2012-03-26
申请人： Eiichi Akahoshi , Mitsuko Ishihara
发明人： Eiichi Akahoshi , Mitsuko Ishihara
CPC分类号： G01N33/5005 , A61K48/005 , A61K49/16 , A61K49/1896 , C07K16/44 , C07K2317/622 , C07K2319/02 , C12N15/85 , G01N33/84
摘要： According to one embodiment, a reporter vector presenting an extracellular binding capacity to metallic compounds contains a nucleotide sequence exhibiting a promoter activity depending on a specific condition, a nucleotide sequence encoding a metallic compound-binding peptide presented extracellularly, and a nucleotide sequence encoding transcription termination signals.
摘要翻译： 根据一个实施方案，向金属化合物呈递细胞外结合能力的报道载体包含根据特定条件显示启动子活性的核苷酸序列，编码胞外呈递的金属化合物结合肽的核苷酸序列和编码转录终止的核苷酸序列 信号。
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9.发明授权Genetically modified animal for use in evaluating harmfulness of test substance 有权用于评估测试物质有害性的转基因动物公开(公告)号：US08247643B2
公开(公告)日：2012-08-21
申请号：US12559303
申请日：2009-09-14
IPC分类号： A01K67/027 , C07H21/04
CPC分类号： A01K67/0275 , A01K2217/052 , A01K2217/206 , A01K2227/105 , A01K2267/0393 , C07K14/4702 , C12N9/0073 , C12N15/85 , C12N15/8509 , C12N2830/008 , C12N2830/15 , C12N2830/30 , C12N2830/85 , G01N21/763 , G01N33/5088
摘要： A genetically modified animal that includes an introduced DNA including a functionally linked aryl hydrocarbon receptor-binding enhancer located 5′-upstream of a tyrosine hydroxylase gene, promoter of any type, reporter gene, and poly(A) addition signal.
摘要翻译： 一种遗传修饰动物，其包括引入的DNA，其包括位于酪氨酸羟化酶基因5'上游的任何功能性连接的芳基烃受体结合增强子，任何类型的启动子，报道基因和聚（A）加成信号。
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公开(公告)号：US20130230872A1
公开(公告)日：2013-09-05
申请号：US13593912
申请日：2012-08-24
申请人： Eiichi AKAHOSHI , Mitsuko Ishihara
发明人： Eiichi AKAHOSHI , Mitsuko Ishihara
IPC分类号： C12Q1/68
CPC分类号： C12N15/63 , C12N15/69 , C12N15/85 , C12Q1/66 , C12Q1/6897
摘要： According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.
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