公开(公告)号：US06399368B1

公开(公告)日：2002-06-04

申请号：US08353940

申请日：1994-12-09

申请人： Elizabeth S. Ward

发明人： Elizabeth S. Ward

IPC分类号： C12N1513

CPC分类号： C07K16/00 ,  A61K47/6827 ,  A61K47/6849 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70535 ,  C07K14/70539 ,  C07K2317/52 ,  C07K2317/53 ,  C07K2319/00

摘要： Variable domain murine T-cell receptor genes have been isolated and used to construct cloning and expression vectors. V&agr;, V&bgr; and single chain V&agr;-V&bgr; fragments have been expressed as secreted domains in Escherichia coli using the vectors. The domains are secreted into the culture supernatant in milligram quantities. The single domains and the single chain T-cell receptors are folded into &bgr;-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted fragments may be useful for immunization to generate anti-clonotypic antibodies, in vaccination or for high resolution structural studies. The genes encoding these domains may also serve as templates for in vitro mutagenesis and improvement of affinities of the TCR fragments for their interaction with cognate peptide-MHC complexes.

摘要翻译： 已经分离了可变结构域鼠T细胞受体基因，并用于构建克隆和表达载体。 Valpha，Vbeta和单链Valpha-Vbeta片段已经使用载体在大肠杆菌中表达为分泌结构域。 以毫克量将结构域分泌到培养上清液中。 单结构域和单链T细胞受体被折叠成类似于免疫球蛋白可变结构域的β-折叠片结构。 分泌的片段可用于免疫以产生抗克隆型抗体，在疫苗接种或高分辨率结构研究中。 编码这些结构域的基因也可以用作体外诱变和提高TCR片段与同源肽-MHC复合物相互作用的亲和力的模板。

公开(公告)号：US20080299618A1

公开(公告)日：2008-12-04

申请号：US12127237

申请日：2008-05-27

申请人： Gregory P. WINTER ,  Elizabeth S. Ward ,  Detlef Gussow

发明人： Gregory P. WINTER ,  Elizabeth S. Ward ,  Detlef Gussow

IPC分类号： C12P21/04 ,  C07K16/00 ,  C07K16/28 ,  C07H21/00 ,  C07K17/00 ,  C12P19/34

CPC分类号： C07K16/461 ,  A61K38/00 ,  C07K2317/24 ,  C12Q1/6876

摘要： The present invention relates to single domain ligands derived from molecules in the immunoglobulin (Ig) superfamily, receptors comprising at least one such ligand, methods for cloning, amplifying and expressing DNA sequences encoding such ligands, preferably using the polymerase chain reaction, methods for the use of said DNA sequences in the productions of Ig-type molecules and said ligands or receptors, and the use of said ligand or receptors in therapy, diagnosis or catalysis.

摘要翻译： 本发明涉及衍生自免疫球蛋白（Ig）超家族中的分子的单结构域配体，包含至少一种这样的配体的受体，用于克隆，扩增和表达编码这种配体的DNA序列的方法，优选使用聚合酶链式反应， 在Ig型分子和所述配体或受体的产生中使用所述DNA序列，以及所述配体或受体在治疗，诊断或催化中的用途。

公开(公告)号：USH875H

公开(公告)日：1991-01-01

申请号：US170211

申请日：1988-03-18

申请人： David J. Ellar ,  Elizabeth S. Ward

发明人： David J. Ellar ,  Elizabeth S. Ward

IPC分类号： C07K14/245 ,  C07K14/325

CPC分类号： C07K14/325 ,  C07K14/245

摘要： Novel nucleic acid fragments coding for insecticidal proteins, the insecticidal proteins encoded thereby, insecticidal compositions containing such proteins, and the use of these proteins in combatting insects, particularly mosquitoes, are described. Chimeric genes containing the novel nucleic acid fragments, and microorganisms, tissues, seeds, and plants incorporating the nucleic acid fragments are also discussed.

公开(公告)号：US06623957B2

公开(公告)日：2003-09-23

申请号：US10162127

申请日：2002-06-04

申请人： Elizabeth S. Ward

发明人： Elizabeth S. Ward

IPC分类号： C12P2104

CPC分类号： C07K16/00 ,  A61K47/6827 ,  A61K47/6849 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70535 ,  C07K14/70539 ,  C07K2317/52 ,  C07K2317/53 ,  C07K2319/00

摘要： Variable domain murine T-cell receptor genes have been isolated and used to construct cloning and expression vectors. V&agr;, V&bgr;, and single chain V&agr;-V&bgr; fragments have been expressed as secreted domains in Escherichia coli using the vectors. The domains are secreted into the culture supernatant in milligram quantities. The single domains and the single chain T-cell receptors are folded into &bgr;-pleated sheet structures similar to those of immunoglobulin variable domains. The secreted fragments may be useful for immunization to generate anti-clonotypic antibodies, in vaccination or for high resolution structural studies. The genes encoding these domains may also serve as templates for in vitro mutagenesis and improvement of affinities of the TCR fragments for their interaction with cognate peptide-MHC complexes.

摘要翻译： 已经分离了可变结构域鼠T细胞受体基因，并用于构建克隆和表达载体。 Valpha，Vbeta和单链Valpha-Vbeta片段已经使用载体在大肠杆菌中表达为分泌结构域。 以毫克量将结构域分泌到培养上清液中。 单结构域和单链T细胞受体被折叠成类似于免疫球蛋白可变结构域的β-折叠片结构。 分泌的片段可用于免疫以产生抗克隆型抗体，在疫苗接种或高分辨率结构研究中。 编码这些结构域的基因也可以用作体外诱变和提高TCR片段与同源肽-MHC复合物相互作用的亲和力的模板。

公开(公告)号：US4918006A

公开(公告)日：1990-04-17

申请号：US824912

申请日：1986-02-03

申请人： David J. Ellar ,  Elizabeth S. Ward

发明人： David J. Ellar ,  Elizabeth S. Ward

IPC分类号： C07K14/325 ,  C12N15/70 ,  C12N15/75

CPC分类号： C12N15/70 ,  C07K14/325 ,  C12N15/75

摘要： Recombinant plasmids containing the larvicidal delta-endotoxin gene were constructed by inserting HindIII fragments of the Bacillus thuringiensis var. israelensis 72-75 Md plasmid into the Escherichia coli vector pUC12. Two recombinants producing a 27-kdal toxin (pIP173 and pIP174) were indentified by screening clones in an E. coli in vitro transcription-translation system. Both recombinants comprised pUC12 and common 9.7 kb HindIII fragment of the B. thuringiensis plasmid. The 27,340 Da polypeptide synthesized in vito from pIP174 transformed into E. coli JM101 and from B. subtilis 168 and spoOJ87 containing the 1.2 kb TaqI fragment from pIP173 was lethal to mosquito larvae.

摘要翻译： 通过插入苏云金芽孢杆菌变种的HindIII片段构建含有杀幼虫δ-内毒素基因的重组质粒。 以色列72-75 Md质粒转入大肠杆菌载体pUC12。 通过在大肠杆菌体外转录翻译系统中筛选克隆来鉴定产生27-kdal毒素（pIP173和pIP174）的两个重组体。 两个重组体都包含苏云金芽孢杆菌质粒的pUC12和9.7Kb的HindIII片段。 从pIP174转化到大肠杆菌JM101和来自枯草芽孢杆菌168的枯草芽孢杆菌168和含有来自pIP173的1.2kb TaqI片段的spoOJ87合成的27,340Da多肽对蚊子幼虫是致死的。