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1.发明授权Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection 失效用于使用粒子浓度化学发光检测改进发光测定的方法和装置公开(公告)号：US06448091B1
公开(公告)日：2002-09-10
申请号：US08335183
申请日：1994-11-07
IPC分类号： G01N2176
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6825 , G01N1/40 , G01N21/69 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N33/585 , G01N2458/30 , C12Q2565/102 , C12Q2563/131 , C12Q2563/103
摘要： Methods and apparatus for performing a binding assay for an analyte of interest present in a sample are described. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物和/或 测定性能物质; 孵育组合物以形成包含颗粒和标记组分的复合物; 在收集区收集复合物; 向收集区引入能够触发标签以使标签发光的触发器; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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2.发明授权Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection 失效用于使用粒子浓度和化学发光检测改善发光测定的装置
公开(公告)号：US5798083A
公开(公告)日：1998-08-25
申请号：US469464
申请日：1995-06-06
IPC分类号： C07H21/00 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/543 , G01N33/58
CPC分类号： G01N21/69 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N33/585 , G01N1/40 , G01N2458/30
摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物和/或 测定性能物质; 孵育组合物以形成包含颗粒和标记组分的复合物; 在收集区收集复合物; 向收集区引入能够触发标签以使标签发光的触发器; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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3.发明授权
公开(公告)号：US5770459A
公开(公告)日：1998-06-23
申请号：US348749
申请日：1994-12-01
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N1/40 , G01N21/66 , G01N21/69 , G01N21/76 , G01N33/533 , G01N33/543 , G01N33/569 , G01N33/58 , G01N33/76 , G01N33/53
CPC分类号： G01N21/69 , C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6825 , C12Q1/686 , G01N21/76 , G01N33/533 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/569 , G01N33/582 , G01N33/585 , G01N33/76 , G01N1/40 , G01N2458/30
摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有所述样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物特异性结合的多个包被的磁性颗粒， /或所述测定性能物质; 孵育所述组合物以形成包含颗粒和所述标记组分的复合物; 在电极表面磁性收集所述复合物; 通过使其与触发器接触来诱导所述标签发光，所述触发器通过在引入电化学能量时转化前体分子而原地形成; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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4.发明授权Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence and chemiluminescence detection 失效用于使用颗粒浓度，化学发光和化学发光检测的电化学产生的改进发光测定的装置
公开(公告)号：US5746974A
公开(公告)日：1998-05-05
申请号：US467028
申请日：1995-06-06
CPC分类号： G01N21/69 , C12Q1/6825 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N1/40 , G01N2458/30
摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有所述样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物特异性结合的多个包被的磁性颗粒， /或所述测定性能物质; 孵育所述组合物以形成包含颗粒和所述标记组分的复合物; 在电极表面磁性收集所述复合物; 通过使其与触发器接触来诱导所述标签发光，所述触发器通过在引入电化学能量时转化前体分子而原地形成; 并测量发射的发光以测量样品中感兴趣的分析物的存在。
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