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公开(公告)号：US20090181858A1
公开(公告)日：2009-07-16
申请号：US11662749
申请日：2005-09-21
Applicant: Alexander Deiters , Ning Wu , Peter G. Schultz , David King , T. Ashton Cropp , Mohua Bose , Dan Groff , Jianming Xie , Eric Brustad
Inventor： Alexander Deiters , Ning Wu , Peter G. Schultz , David King , T. Ashton Cropp , Mohua Bose , Dan Groff , Jianming Xie , Eric Brustad
IPC: C40B30/06 , C12N9/00 , C07K2/00 , C07H21/00 , C12Q1/68 , C12P21/00 , C40B40/06 , C40B40/02 , C40B30/00
CPC classification number: C12N9/93
Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, Ome-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.
Abstract translation: 生成蛋白质生物合成机理成分的组合物和方法，包括正交亮氨酰-tRNA，正交亮氨酰 - 氨基-tRNA合成酶和亮氨酰-tRNAs /合成酶的正交对，其结合光调节氨基酸，OMe-L-酪氨酸，α-氨基辛酸 酸或O-硝基苄基半胱氨酸转化成蛋白质是响应于琥珀选择密码子提供的蛋白质。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生具有光调节氨基酸，ω-L-酪氨酸，α-氨基辛酸或邻硝基苄基半胱氨酸的蛋白质的方法。
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公开(公告)号：US20090148887A1
公开(公告)日：2009-06-11
申请号：US12262025
申请日：2008-10-30
Applicant: ERIC BRUSTAD , MARK L. BUSHEY , PETER G. SCHULTZ
Inventor： ERIC BRUSTAD , MARK L. BUSHEY , PETER G. SCHULTZ
CPC classification number: C12P13/222 , C07K17/06 , C12N9/93 , C12N15/67 , C12P21/02
Abstract: Provided are compositions comprising an aminoacyl tRNA synthetase that selectively recognizes a boronic amino acid. Methods of incorporating a boronic amino acid into a target polypeptides and target polypeptides produced by the methods are also provided. Methods of producing a protein, which methods comprise site-specifically encoding a boronic amino acid residue into a mutant protein and selectively converting the boronic amino acid residue into a natural amino acid residue are provided. Also provided are compositions comprising a solid phase matrix covalently bound to a polypeptide through a boronic amino acid residue. In addition, compositions comprising a purified population of polypeptide molecules that each comprise a borono amino acid at a selected site are provided.
Abstract translation: 提供了包含选择性识别硼酸氨基酸的氨酰基tRNA合成酶的组合物。 还提供了将硼酸氨基酸掺入靶多肽和通过该方法产生的靶多肽的方法。 提供了产生蛋白质的方法，该方法包括将硼酸氨基酸残基特异性编码到突变蛋白中并选择性地将硼酸氨基酸残基转化成天然氨基酸残基。 还提供了包含通过硼酸氨基酸残基与多肽共价结合的固相基质的组合物。 此外，提供了包含纯化的多肽分子群的组合物，其各自包含选定位点的硼氢氨基酸。
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公开(公告)号：US20060134746A1
公开(公告)日：2006-06-22
申请号：US11233508
申请日：2005-09-21
Applicant: Alexander Deiters , Ning Wu , Peter Schultz , David King , T. Cropp , Mohua Bose , Dan Groff , Jianming Xie , Eric Brustad
Inventor： Alexander Deiters , Ning Wu , Peter Schultz , David King , T. Cropp , Mohua Bose , Dan Groff , Jianming Xie , Eric Brustad
CPC classification number: C12N9/93
Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.
Abstract translation: 生成蛋白质生物合成机理成分的组合物和方法，包括正交亮氨酰-tRNA，正交亮氨酰 - 氨基-tRNA合成酶和亮氨酰-tRNAs /合成酶的正交对，其结合光调节氨基酸，OMe-L-酪氨酸，α-氨基辛酸 酸或O-硝基苄基半胱氨酸进入蛋白质是响应于琥珀选择密码子而提供的。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有光调节氨基酸，OMe-L-酪氨酸，α-氨基辛酸或邻硝基苄基半胱氨酸的蛋白质的方法。
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