Abstract:
The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.
Abstract:
Sidestream smoke collection apparatus comprises a smoking chamber 14 including a plenum chamber 24, air inlets 28, an internal wall 26 having slits 30 for the passage of air, a cigarette holder 22 above the internal wall 26, an outlet pipe 16 above the cigarette holder, an upper deflector 38 between the cigarette holder and the outlet and a lower deflector 32 between the cigarette holder and the internal wall. This dispatch of air inlet and outlet and deflectors allows more efficient collection of sidestream smoke.
Abstract:
The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.
Abstract translation:本发明涉及一种突变体,非天然存在或转基因植物细胞,其包含:(i)至少一种多核苷酸,其包含,组成或基本上由编码异丙基苹果酸合酶的序列组成,并且与SEQ ID NO具有至少60%的序列同一性 :1或SEQ ID NO:10或SEQ ID NO:12或SEQ ID NO:14; 或(ii)由所述多核苷酸编码的多肽; 或(iii)与SEQ ID NO:2或SEQ ID NO:11或SEQ ID NO:13或SEQ ID NO:15具有至少60%序列同一性的多肽; 或(iv)包含所述多核苷酸序列的构建体,载体或表达载体,任选其中所述构建体,载体或表达载体另外包含启动子,其包含SEQ ID NO:8所示的序列或基本上由SEQ ID NO: 其与其至少约60%同一性的变体或毛发启动子。