公开(公告)号：US20080007820A1

公开(公告)日：2008-01-10

申请号：US11806458

申请日：2007-05-31

Applicant: Gunter Moehler ,  Mirko Liedtke

Inventor： Gunter Moehler ,  Mirko Liedtke

IPC: G02B21/36 ,  H04L12/28

CPC classification number: H04L12/407 ,  G02B21/008 ,  G02B21/365 ,  H04L2012/40215

Abstract: Specimen laser-scanning microscope with rasterizing illumination and detector resources (resources), which rasterizes and scan detects a specimen. A real-time control device (device) performs synchronous reading-out with the rasterizing pixel cycle. A data port serially communicates with the device using a bidirectional high-speed data stream and with the resources via a serial, bidirectional high-speed data stream with a data conversion to/from parallel to serial. The high-speed data stream is made up of data packets with data bits and type bits and no additional header or protocol bits. The data bits contain data from/on the resources and the type bits code the type of data. Type information is stored in the resources as well as the device. The type information defines processing functions for data types coded by the type bits, and the resources and/or the device determine the data type using type bits and process data coded in the data bits.

Abstract translation: 具有光栅照明和检测器资源（资源）的样品激光扫描显微镜，其光栅化和扫描检测样品。 实时控制装置（装置）利用光栅化像素周期执行同步读出。 数据端口使用双向高速数据流和通过串行双向高速数据流与资源串行地与设备进行通信，数据转换到并行到串行数据转换。 高速数据流由具有数据位和类型位的数据分组组成，没有附加的报头或协议位。 数据位包含来自资源的数据，类型位代码数据类型。 类型信息存储在资源以及设备中。 类型信息定义由类型位编码的数据类型的处理功能，并且资源和/或设备使用在数据位中编码的类型位和处理数据来确定数据类型。

公开(公告)号：US20060000984A1

公开(公告)日：2006-01-05

申请号：US11182465

申请日：2005-07-15

Applicant: Ralf Wolleschensky ,  Gunter Moehler ,  Volker Gerstner

Inventor： Ralf Wolleschensky ,  Gunter Moehler ,  Volker Gerstner

IPC: G01J1/58 ,  F21V9/16

CPC classification number: G02B21/0004 ,  G02B21/16

Abstract: A method of optical detection of characteristic quantities of an illuminated specimen comprising detecting a signal that is backscattered, reflected and/or fluoresced and/or transmitted from the specimen by a spatially resolving detector wherein radiation coming from the specimen is imaged on the detector, shifting the position of the radiation which is measured in a spatially resolved manner relative to the detector and determining intermediate values by an algorithm from the signals measured in different shifts for purposes of increasing the spatial resolution of the detector. An arrangement for performing the method is also disclosed.

公开(公告)号：US06747737B2

公开(公告)日：2004-06-08

申请号：US09895518

申请日：2001-06-28

Applicant: Ralf Wolleschensky ,  Gunter Moehler

Inventor： Ralf Wolleschensky ,  Gunter Moehler

IPC: G01J328

CPC classification number: G02B21/002 ,  G02B21/06

Abstract: In light detection systems for illuminating specimens having a plurality of detection channels, particularly channels employing laser scanning microscopes, an improved method comprises assigning an upper and/or lower limiting value which is adjustable for at least one channel and changing the channel to be detected with respect to its mode of operation when the limiting value is reached.

Abstract translation: 在用于照射具有多个检测通道的样品的光检测系统中，特别是使用激光扫描显微镜的通道，改进的方法包括分配对于至少一个通道可调节的上限值和/或下限值，并且用 当达到极限值时，其操作模式。

公开(公告)号：US6037583A

公开(公告)日：2000-03-14

申请号：US010079

申请日：1998-01-21

Applicant: Gunter Moehler ,  Guenter Schoeppe ,  Sebastian Tille

Inventor： Gunter Moehler ,  Guenter Schoeppe ,  Sebastian Tille

IPC: G02B21/00 ,  H01J3/14

CPC classification number: G02B21/002

Abstract: A control system for a scanner is disclosed, especially for a laser scanning microscope, with an oscillating motor for driving an oscillating mirror serving for the linearly oscillating deflection of a beam bundle, with a control unit for supplying the oscillating motor with an exciting current which is variable with respect to the control frequency, frequency curve, and amplitude, with a function generator which is connected with the control unit, and with a measurement value transducer for obtaining a sequence of information about the deflection positions of the oscillating mirror.The measurement value transducer is connected with the function generator by way of a logic unit for determining correction values for the exciting current. Accordingly, it is advantageously possible, by evaluating the information supplied by the measurement value transducer about the actual deflection position of the oscillating mirror, to determine correction values with the assistance of the logic unit. These correction values can be used, in turn, to influence the control frequencies emitted by the function generator in such a way that deviations are minimized or completely prevented.

Abstract translation: 公开了一种用于扫描仪的控制系统，特别是用于激光扫描显微镜，具有用于驱动用于束束的线性振荡偏转的振荡镜的振荡电机，具有用于向振荡电机供应励磁电流的控制单元 相对于控制频率，频率曲线和幅度可变，与控制单元连接的函数发生器，以及用于获得关于振荡反射镜的偏转位置的信息序列的测量值传感器。 测量值传感器通过逻辑单元与功能发生器连接，用于确定激励电流的校正值。 因此，通过评估由测量值传感器提供的关于振荡镜的实际偏转位置的信息，有利于在逻辑单元的协助下确定校正值是有利的。 可以使用这些校正值，从而以使偏差最小化或完全防止的方式影响函数发生器发出的控制频率。

公开(公告)号：US07859673B2

公开(公告)日：2010-12-28

申请号：US11822152

申请日：2007-07-02

Applicant: Gunter Moehler ,  Dietmar Schmidt ,  Mirko Liedtke

Inventor： Gunter Moehler ,  Dietmar Schmidt ,  Mirko Liedtke

IPC: G01N21/55 ,  H03K19/14

CPC classification number: G01J1/44 ,  G01J1/42 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16

Abstract: A method for detecting and analyzing light signals, in which a light signal impinges an optoelectric converter, where it is converted into an electric signal and the electric signal subsequent to the conversion is distributed into several analysis channels (13.1-13.4), within each analysis channel (13.1), (i) a signal analysis is performed, which is different from the signal analysis for the other analysis channels (13.2-13.4) and (ii) an output signal is created. In such a method, one or more output signals are selected for further processing and output using a specified, changeable selection criterion.

Abstract translation: 一种用于检测和分析其中光信号照射光电转换器的光信号的方法，其中它被转换为电信号，并且在转换之后的电信号被分配到多个分析通道（13.1-13.4）中，在每个分析 通道（13.1），（i）进行与其他分析通道（13.2-13.4）的信号分析不同的信号分析，（ii）产生输出信号。 在这种方法中，选择一个或多个输出信号用于使用指定的可变选择标准进一步处理和输出。

公开(公告)号：US07145652B2

公开(公告)日：2006-12-05

申请号：US10419016

申请日：2003-04-17

Applicant: Gunter Moehler ,  Ralf Wolleschensky ,  Mirko Liedtke

Inventor： Gunter Moehler ,  Ralf Wolleschensky ,  Mirko Liedtke

IPC: G01N15/02 ,  G02B21/00 ,  G01J3/28

CPC classification number: G02B21/06 ,  G02B26/007

Abstract: A microscope with optical components which are provided in the illumination beam path and/or observation beam path and/or detection beam path for exerting different spectral influences on at least one of the beam paths mentioned above. An arrangement comprising a light source emitting a plurality of wavelengths and at least one spectral sensor detecting the light of this light source after interacting with a component are provided, preferably outside these beam paths. The interaction is carried out by transmission or reflection of the light by the component. The detected values of the spectral sensor are compared to pre-stored values and correlation with pre-stored values is advantageously carried out in a storage. The correlation is carried out together with a position signal of a component changer. The detected values are stored separately and together with a position signal of a component changer when there is no correlation with pre-stored values.

公开(公告)号：US06744576B2

公开(公告)日：2004-06-01

申请号：US10049802

申请日：2002-06-19

Applicant: Gunter Moehler

Inventor： Gunter Moehler

IPC: G02B716

CPC classification number: G02B21/248 ,  G02B7/16

Abstract: A method for detecting the maximum quantity of possible positions of an exchangeable nosepiece or slide in a microscope system is disclosed. The method comprises the steps of: starting from an initial position which corresponds to a first position, adjusting the maximum position; comparing the maximum position to a position registered in a memory; and storing the results of the comparison.

Abstract translation: 公开了一种用于检测显微镜系统中可更换的鼻梁或滑块的最大可能位置量的方法。 该方法包括以下步骤：从对应于第一位置的初始位置开始，调整最大位置; 将最大位置与登记在存储器中的位置进行比较; 并存储比较结果。

公开(公告)号：US06462345B1

公开(公告)日：2002-10-08

申请号：US09295556

申请日：1999-04-21

Applicant: Ulrich Simon ,  Sebastian Tille ,  Gunter Moehler ,  Stefan Wilhelm ,  Ulrich Meisel ,  Ernst Hans Karl Stelzer

Inventor： Ulrich Simon ,  Sebastian Tille ,  Gunter Moehler ,  Stefan Wilhelm ,  Ulrich Meisel ,  Ernst Hans Karl Stelzer

IPC: G01N2164

CPC classification number: G02B21/0056 ,  G01N21/645 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/0084

Abstract: A process for confocal microscopy is disclosed in which laser light is coupled into a microscope beam path, directed successively with respect to time onto different locations of a specimen, and an image of the scanned plane is generated from the light reflected and emitted by the irradiated locations. A change in the spectral composition and in the intensity of light is carried out during the deflection of the laser beam from location to location, while the deflection continues in an uninterrupted manner. In this way, at least two locations of the specimen located next to one another are acted upon by light with different spectral characteristics and by laser radiation of different intensity. By periodically interrupting the coupling in of the laser light during the deflection of the microscope beam path, it is made possible that only selected portions of the image field are acted upon by the laser radiation. A laser scanning microscope for carrying out this process is also disclosed.

Abstract translation: 公开了一种用于共焦显微镜的方法，其中将激光耦合到相对于时间相对于样本的不同位置的显微镜光束路径中，并且从被照射的反射和发射的光产生扫描平面的图像 位置。 在激光束从位置到位置的偏转期间进行光谱组成和光强度的变化，而偏转以不间断的方式继续。 以这种方式，彼此相邻的样本的至少两个位置由具有不同光谱特性的光和不同强度的激光辐射作用。 通过在显微镜光束路径的偏转期间周期性地中断激光的耦合，使得只有图像场的选定部分被激光辐射作用才有可能。 还公开了用于进行该方法的激光扫描显微镜。

公开(公告)号：US20090228618A1

公开(公告)日：2009-09-10

申请号：US12379193

申请日：2009-02-13

Applicant: Andreas Kuehm ,  Nico Presser ,  Gunter Moehler

Inventor： Andreas Kuehm ,  Nico Presser ,  Gunter Moehler

IPC: G06F3/00

CPC classification number: G06F13/4059

Abstract: A peripheral interface and process for data transfer, especially for laser scanning microscopes. The peripheral interface permits a gap-free transfer of data with high transmission speed at low cost and using a non-real-time-enabled operating system of the control computer. In a peripheral interface having a connection for a system bus of a control computer, a peripheral connection for a peripheral device and a control unit serving for one-way transmission of a predetermined amount of data from the control computer to the peripheral device and/or vice versa accesses via the system bus, a work memory region of the control computer serves as buffers preassigned to it, where the control unit prepares for the control computer a progress report of the transfer for retrieval and the control unit of the control computer is informed of the progress of the processing of the buffer independently of the transfer, in which case it allows for the progress of the processing when accessing the buffer. The peripheral device can be part of a laser scanning microscope.

Abstract translation: 用于数据传输的外围接口和过程，尤其适用于激光扫描显微镜。 外围接口允许以低成本传输高传输速率的数据，并使用控制计算机的非实时启用操作系统。 在具有用于控制计算机的系统总线的连接的外围接口中，用于外围设备的外围连接和用于从控制计算机向外围设备单向传输预定数量的数据的控制单元和/或 反过来通过系统总线访问，控制计算机的工作存储器区域用作预先分配给它的缓冲器，其中控制单元为控制计算机准备用于检索的传送的进度报告，并且控制计算机的控制单元被通知 独立于传输处理缓冲区的进度，在这种情况下，它允许在访问缓冲区时进行处理。 外围设备可以是激光扫描显微镜的一部分。

公开(公告)号：US07271897B2

公开(公告)日：2007-09-18

申请号：US11182465

申请日：2005-07-15

Applicant: Ralf Wolleschensky ,  Gunter Moehler ,  Volker Gerstner

Inventor： Ralf Wolleschensky ,  Gunter Moehler ,  Volker Gerstner

IPC: G01J3/30 ,  G01N21/00

CPC classification number: G02B21/0004 ,  G02B21/16

Abstract: A method of optical detection of characteristic quantities of an illuminated specimen comprising detecting a signal that is backscattered, reflected and/or fluoresced and/or transmitted from the specimen by a spatially resolving detector wherein radiation coming from the specimen is imaged on the detector, shifting the position of the radiation which is measured in a spatially resolved manner relative to the detector and determining intermediate values by an algorithm from the signals measured in different shifts for purposes of increasing the spatial resolution of the detector. An arrangement for performing the method is also disclosed.

Abstract translation: 一种光学检测照明样本的特征量的方法，包括通过空间分辨检测器检测从样品反向散射，反射和/或发射和/或发射和/或发射的信号，其中来自样品的辐射被成像在检测器上，移位 以相对于检测器的空间分辨方式测量的辐射的位置，并且为了增加检测器的空间分辨率，由不同位移测量的信号通过算法确定中间值。 还公开了一种用于执行该方法的装置。