公开(公告)号：US07411060B2

公开(公告)日：2008-08-12

申请号：US10717573

申请日：2003-11-21

申请人： Jen-Leih Wu ,  Guor Mour Her

发明人： Jen-Leih Wu ,  Guor Mour Her

IPC分类号： C07H21/04 ,  A01K67/033

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/058 ,  A01K2227/40 ,  A01K2267/03 ,  C07K14/461 ,  C07K14/475 ,  C12N15/8509

摘要： The present invention relates to expression control sequences of a vertebrate liver fatty acid binding protein (L-FABP) gene that, when operably linked to a reporter (e.g., a heterologous reporter, such as the green fluorescent protein (GFP)), directly express the reporter in a fashion that mimics the liver-specific development of the L-FABP gene in the vertebrate.

摘要翻译： 本发明涉及当与报告物（例如，异源报告物，例如绿色荧光蛋白（GFP））可操作地连接时，脊椎动物肝脏脂肪酸结合蛋白（L-FABP）基因的表达控制序列直接表达 记者以模仿L-FABP基因在脊椎动物肝脏特异性发育的方式。

公开(公告)号：US08383880B2

公开(公告)日：2013-02-26

申请号：US12705509

申请日：2010-02-12

申请人： Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

发明人： Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

IPC分类号： A01K67/027 ,  C12N15/00

CPC分类号： A01K67/0275 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C07K2319/60 ,  C12N9/0028 ,  C12N15/8509 ,  C12N2800/90

摘要： A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.

摘要翻译： 公开了在转基因鱼中诱导雄性和/或雌性不孕症的方法。 还公开了产生具有感兴趣的表型和/或基因型的不育GM鱼的方法。 该方法涉及产生GM基因组鱼，其基因组包含可操作地连接于选自卵巢特异性启动子和睾丸特异性启动子的鱼性腺特异性启动子的外源转基因。 转基因包含选自还原酶和光敏剂的自杀基因，其中还原酶可操作地连接到报告基因。 如果用有效量的还原酶激活的细胞毒性前药处理表达还原酶的GM鱼，或者如果用光照射处理表达光敏剂的GM鱼，则诱导GM鱼的不育。

公开(公告)号：US20100212039A1

公开(公告)日：2010-08-19

申请号：US12705509

申请日：2010-02-12

申请人： JEN-LEIH WU ,  SHAO-YANG HU ,  GUOR MOUR HER

发明人： JEN-LEIH WU ,  SHAO-YANG HU ,  GUOR MOUR HER

IPC分类号： A01K67/027 ,  A01K67/02

CPC分类号： A01K67/0275 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C07K2319/60 ,  C12N9/0028 ,  C12N15/8509 ,  C12N2800/90

摘要： A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.

摘要翻译： 公开了在转基因鱼中诱导雄性和/或雌性不孕症的方法。 还公开了产生具有感兴趣的表型和/或基因型的不育GM鱼的方法。 该方法涉及产生GM基因组鱼，其基因组包含可操作地连接于选自卵巢特异性启动子和睾丸特异性启动子的鱼性腺特异性启动子的外源转基因。 转基因包含选自还原酶和光敏剂的自杀基因，其中还原酶可操作地连接到报告基因。 如果用有效量的还原酶激活的细胞毒性前药处理表达还原酶的GM鱼，或者如果用光照射处理表达光敏剂的GM鱼，则诱导GM鱼的不育。