公开(公告)号：US07411060B2

公开(公告)日：2008-08-12

申请号：US10717573

申请日：2003-11-21

申请人： Jen-Leih Wu ,  Guor Mour Her

发明人： Jen-Leih Wu ,  Guor Mour Her

IPC分类号： C07H21/04 ,  A01K67/033

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/058 ,  A01K2227/40 ,  A01K2267/03 ,  C07K14/461 ,  C07K14/475 ,  C12N15/8509

摘要： The present invention relates to expression control sequences of a vertebrate liver fatty acid binding protein (L-FABP) gene that, when operably linked to a reporter (e.g., a heterologous reporter, such as the green fluorescent protein (GFP)), directly express the reporter in a fashion that mimics the liver-specific development of the L-FABP gene in the vertebrate.

摘要翻译： 本发明涉及当与报告物（例如，异源报告物，例如绿色荧光蛋白（GFP））可操作地连接时，脊椎动物肝脏脂肪酸结合蛋白（L-FABP）基因的表达控制序列直接表达 记者以模仿L-FABP基因在脊椎动物肝脏特异性发育的方式。

公开(公告)号：US08383880B2

公开(公告)日：2013-02-26

申请号：US12705509

申请日：2010-02-12

申请人： Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

发明人： Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

IPC分类号： A01K67/027 ,  C12N15/00

CPC分类号： A01K67/0275 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C07K2319/60 ,  C12N9/0028 ,  C12N15/8509 ,  C12N2800/90

摘要： A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.

摘要翻译： 公开了在转基因鱼中诱导雄性和/或雌性不孕症的方法。 还公开了产生具有感兴趣的表型和/或基因型的不育GM鱼的方法。 该方法涉及产生GM基因组鱼，其基因组包含可操作地连接于选自卵巢特异性启动子和睾丸特异性启动子的鱼性腺特异性启动子的外源转基因。 转基因包含选自还原酶和光敏剂的自杀基因，其中还原酶可操作地连接到报告基因。 如果用有效量的还原酶激活的细胞毒性前药处理表达还原酶的GM鱼，或者如果用光照射处理表达光敏剂的GM鱼，则诱导GM鱼的不育。

公开(公告)号：US07670836B2

公开(公告)日：2010-03-02

申请号：US11806234

申请日：2007-05-30

申请人： Jenn-Kan Lu ,  Jen-Leih Wu ,  Tze-Ting Chiou

发明人： Jenn-Kan Lu ,  Jen-Leih Wu ,  Tze-Ting Chiou

IPC分类号： C07H17/00 ,  C07K14/00

CPC分类号： A61K45/06 ,  A01K2217/05 ,  A61K38/17 ,  C07K14/43509 ,  A61K2300/00

摘要： The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G(−) and G(+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.

摘要翻译： 本发明提供了一种抗微生物肽单链，其由对白对虾分离和纯化，并且能够通过异源表达系统如酵母中的分子克隆技术大规模生产。 Monodoncin对G（ - ）和G（+）细菌以及杀真菌活性具有广泛的抑菌和杀菌作用，可与常规抗生素联合使用，作为“鸡尾酒疗法”，以提高常规抗菌素的治疗效果 抗生素。

公开(公告)号：US06630456B2

公开(公告)日：2003-10-07

申请号：US10080656

申请日：2002-02-25

申请人： Jen-Leih Wu ,  Jiann-Ruey Hong

发明人： Jen-Leih Wu ,  Jiann-Ruey Hong

IPC分类号： A61A31713

CPC分类号： A61K38/162 ,  C12Q1/6897

摘要： The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabirnavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGEP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection.that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMcl-1a protein.

摘要翻译： 本发明提供了通过用水禽病毒特别是感染性胰腺坏死病毒（IPNV）感染水生生物来研究水生生物细胞凋亡的机制。 在Chinook鲑鱼胚胎细胞（CHSE-214）等水生细胞中IPNV的感染将细胞转化为凋亡细胞。 本发明还提供了一种通过将EGFP（变异型GFP）克隆到水生细胞并通过显微技术监测荧光来监测细胞凋亡过程中的形态学变化的方法。 荧光强度反映细胞中EGEP的表达。 最后，本发明提供了诱导或预防/抢救包括水生和脊椎动物在内的宿主细胞凋亡的方法。 凋亡可以通过IPNV感染或VP3转染诱导，VP3是从IPNV片段A衍生的32kDa蛋白质。可通过反义VP3 RNA或zfMcl-1a蛋白质预防或拯救细胞凋亡。

公开(公告)号：US06235874B1

公开(公告)日：2001-05-22

申请号：US09383212

申请日：1999-08-26

申请人： Jen-Leih Wu ,  Jyh-Yih Chen

发明人： Jen-Leih Wu ,  Jyh-Yih Chen

IPC分类号： C07K1465

CPC分类号： C07K14/65

摘要： The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH2—B—C—A—D—COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II cDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells. The isolated and purified IGF-II E domain peptide has mitogenic and anti-tumor activity.

摘要翻译： 本发明涉及可以在细胞中克隆和表达的鱼类胰岛素样生长因子II（IGF-II）cDNA的发现和构建。 本发明还涉及通过使用鱼IGF-II cDNA的基因表达系统生产生物活性鱼IGF-II多肽。 鱼IGF-II cDNA具有1977bp，其转录成前肽（信号肽），以及B，C，A，D，E结构域肽。 鱼成熟IGF-II是含有NH2-B-C-A-D-COOH结构域的单一多肽。 成熟的IGF-II多肽的重量为7kDa，具有70个氨基酸。 鱼重组IGF-II cDNA可以克隆并表达在大肠杆菌，酵母，杆状病毒和鱼细胞中。 分离和纯化的IGF-II E结构域肽具有促有丝分裂和抗肿瘤活性。

公开(公告)号：US6010882A

公开(公告)日：2000-01-04

申请号：US3708

申请日：1998-01-07

申请人： Jen-Leih Wu ,  Jyh-Yih Chen ,  Chi-Yao Chang

发明人： Jen-Leih Wu ,  Jyh-Yih Chen ,  Chi-Yao Chang

IPC分类号： C07K14/65 ,  C12N15/12 ,  C12N15/18 ,  C12N15/70 ,  C12N15/79 ,  C12N15/81 ,  C12N15/86 ,  C12N5/00 ,  C12N15/63

CPC分类号： C07K14/65

摘要： The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH.sub.2 -B-C-A-D-COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II CDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells.

摘要翻译： 本发明涉及可以在细胞中克隆和表达的鱼类胰岛素样生长因子II（IGF-II）cDNA的发现和构建。 本发明还涉及通过使用鱼IGF-II cDNA的基因表达系统生产生物活性鱼IGF-II多肽。 鱼IGF-II cDNA具有1977bp，其转录成前肽（信号肽），以及B，C，A，D，E结构域肽。 鱼成熟IGF-II是含有NH2-B-C-A-D-COOH结构域的单一多肽。 成熟的IGF-II多肽的重量为7kDa，具有70个氨基酸。 鱼重组IGF-II CDNA可以在大肠杆菌，酵母，杆状病毒和鱼细胞中克隆并表达。

公开(公告)号：US20080032385A1

公开(公告)日：2008-02-07

申请号：US11806234

申请日：2007-05-30

申请人： Jenn-Kan Lu ,  Jen-Leih Wu ,  Tze-Ting Chiou

发明人： Jenn-Kan Lu ,  Jen-Leih Wu ,  Tze-Ting Chiou

IPC分类号： C12N1/11 ,  C07H21/04 ,  C12N15/00

CPC分类号： A61K45/06 ,  A01K2217/05 ,  A61K38/17 ,  C07K14/43509 ,  A61K2300/00

摘要： The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G (−) and G (+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.

摘要翻译： 本发明提供了一种抗微生物肽单链，其由对白对虾分离和纯化，并且能够通过异源表达系统如酵母中的分子克隆技术大规模生产。 Monodoncin对G（ - ）和G（+）细菌以及杀真菌活性表现出广泛的抑菌和杀菌作用，可与常规抗生素联合使用，作为“鸡尾酒疗法”，以改善常规治疗效果 抗生素。

公开(公告)号：US07008621B2

公开(公告)日：2006-03-07

申请号：US10393268

申请日：2003-03-21

申请人： Jen-Leih Wu ,  Jiann-Ruey Hong

发明人： Jen-Leih Wu ,  Jiann-Ruey Hong

IPC分类号： A01N55/00 ,  C12N7/00 ,  C12N9/00 ,  A01K61/00

CPC分类号： A61K38/162 ,  C12Q1/6897

摘要： The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabirnavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGFP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMcl-1a protein.

摘要翻译： 本发明提供了通过用水禽病毒特别是感染性胰腺坏死病毒（IPNV）感染水生生物来研究水生生物细胞凋亡的机制。 在Chinook鲑鱼胚胎细胞（CHSE-214）等水生细胞中IPNV的感染将细胞转化为凋亡细胞。 本发明还提供了一种通过将EGFP（变异型GFP）克隆到水生细胞并通过显微技术监测荧光来监测细胞凋亡过程中的形态学变化的方法。 荧光强度反映细胞中EGFP的表达。 最后，本发明提供了诱导或预防/抢救包括水生和脊椎动物在内的宿主细胞凋亡的方法。 可以通过IPNV感染或VP3转染诱导凋亡，VP3是来自IPNV片段A的32kDa蛋白。可以通过反义VP3 RNA或zfMcl-1a蛋白来预防或拯救细胞凋亡。

公开(公告)号：US06207817B1

公开(公告)日：2001-03-27

申请号：US09414439

申请日：1999-10-07

申请人： Jen-Leih Wu ,  Jyh-Yih Chen

发明人： Jen-Leih Wu ,  Jyh-Yih Chen

IPC分类号： C07H2104

CPC分类号： C07K14/65 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/80

摘要： This present invention relates to the findings of the DNA sequences of fish insulin-like growth factor II (IGF-II) promoter regions and recombinant IGF-II promoters. These DNA sequences are capable of being expressed in eukaryotic cells and fish embryos of another fish species. The integration of the IGF-II promoter regions or recombinant IGF-II promoters into fish of another species results in the creation of a transgenic fish. The results of this invention illustrate that a fish IGF-II promoter not only can act as a growth factor to stimulate the growth and development of fish, but also is capable of being expressed in other eukaryotic cells such as in human cells.

摘要翻译： 本发明涉及鱼类胰岛素样生长因子II（IGF-II）启动子区和重组IGF-II启动子的DNA序列的发现。 这些DNA序列能够在真核细胞和另一种鱼类的鱼胚中表达。 将IGF-II启动子区域或重组IGF-II启动子整合到另一物种的鱼中导致产生转基因鱼。 本发明的结果说明，鱼IGF-II启动子不仅可以作为刺激鱼的生长和发育的生长因子，而且能够在其它真核细胞如人细胞中表达。