公开(公告)号：US20150152463A1

公开(公告)日：2015-06-04

申请号：US14609603

申请日：2015-01-30

申请人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

发明人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

IPC分类号： C12P21/00

CPC分类号： C12P21/005 ,  C12N9/0071 ,  C12N9/1051 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2510/04 ,  C12Y114/18002

摘要： The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.

摘要翻译： 本发明提供缺乏CMP-Neu5Ac羟化酶（Cmah）和/或糖蛋白α-1,3-半乳糖基转移酶（Ggta1）的非人哺乳动物细胞系。 还提供了使用本文公开的细胞来生产具有人类糖基化模式的重组蛋白质的方法。

公开(公告)号：US4673641A

公开(公告)日：1987-06-16

申请号：US573642

申请日：1984-01-25

申请人： Henry J. George ,  Richard A. Krzyzek ,  Lynn W. Enquist ,  Roger J. Watson

发明人： Henry J. George ,  Richard A. Krzyzek ,  Lynn W. Enquist ,  Roger J. Watson

IPC分类号： A61K39/00 ,  A61K39/245 ,  C12N15/66 ,  C12N15/70 ,  C12P21/04 ,  C12P21/00 ,  C07K3/24 ,  C12N1/00 ,  C12N1/02 ,  C12N15/00 ,  C12P21/02

CPC分类号： C12N15/70 ,  C12N15/66 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/61 ,  C07K2319/75

摘要： Methods are provided for stabilization and purification of proteins produced in host cell systems. The methods provide for significant stabilization of any unfused non-bacterial protein that is produced in the same host cell that is producing an aggregate-forming fusion protein or any aggregate-forming protein.

摘要翻译： 提供了用于稳定和纯化在宿主细胞系统中产生的蛋白质的方法。 所述方法提供在产生聚集体形成融合蛋白或任何聚集体形成蛋白质的相同宿主细胞中产生的任何未融合的非细菌蛋白质的显着稳定化。

公开(公告)号：US09062338B1

公开(公告)日：2015-06-23

申请号：US14609603

申请日：2015-01-30

申请人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

发明人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

IPC分类号： C12P21/00

CPC分类号： C12P21/005 ,  C12N9/0071 ,  C12N9/1051 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2510/04 ,  C12Y114/18002

摘要： The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.

摘要翻译： 本发明提供缺乏CMP-Neu5Ac羟化酶（Cmah）和/或糖蛋白α-1,3-半乳糖基转移酶（Ggta1）的非人哺乳动物细胞系。 还提供了使用本文公开的细胞来生产具有人类糖基化模式的重组蛋白质的方法。

公开(公告)号：US20130004992A1

公开(公告)日：2013-01-03

申请号：US13538825

申请日：2012-06-29

申请人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

发明人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

IPC分类号： C12N5/10 ,  C12P21/00

CPC分类号： C12P21/005 ,  C12N9/0071 ,  C12N9/1051 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2510/04 ,  C12Y114/18002

摘要： The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.

公开(公告)号：US06623981B2

公开(公告)日：2003-09-23

申请号：US09237061

申请日：1999-01-26

申请人： Jeffrey T. Billheimer ,  Dietmar A. Seiffert ,  Leah A. Breth ,  Timothy C. Burn ,  Ira B. Dicker ,  Henry J. George ,  Gregory F. Hollis ,  Jeannine M. Hollis ,  Jennifer E. Kochie ,  Karyn T. O'Neil

发明人： Jeffrey T. Billheimer ,  Dietmar A. Seiffert ,  Leah A. Breth ,  Timothy C. Burn ,  Ira B. Dicker ,  Henry J. George ,  Gregory F. Hollis ,  Jeannine M. Hollis ,  Jennifer E. Kochie ,  Karyn T. O'Neil

IPC分类号： G01N33553

CPC分类号： G01N33/6893 ,  C07K14/70557 ,  C07K16/2848 ,  G01N33/566 ,  G01N33/68 ,  G01N2333/70546 ,  G01N2500/00 ,  Y10S435/968 ,  Y10S436/811

摘要： This invention relates to the detection of patients at risk for developing integrin antagonist/agonist mediated disease states. This invention relates to assays useful for the detection in a patient bodily fluid sample of drug-dependent antibodies which bind to integrins, or intergrin-associated proteins or complexes thereof in the presence of an integrin antagonist/agonist. This invention also relates to assays useful for the detection in a patient bodily fluid sample of drug-dependent antibodies (DDABS) that bind to integrins, including the platelet glycoprotein IIb/IIIa (GPIIb/IIIa), in the presence of a integrin agonist and/or antagonist. This invention also relates to procedures for identifying integrin antagonists/agonists that are less prone to elicit integrin antagonist/agonist mediated disease states. This invention also relates to procedures which increase the recovery of integrin-directed antibodies in body fluids, resulting in an increased sensitivity and specificity of DDAB detection assays. This invention also relates to procedures for treating blood samples, which dissociate antibodies to GPIIb/IIIa from the platelet surface, thereby increasing the recovery from the platelet supernatant. This invention also relates to the use of different GPIIb/IIIa preparations to identify patients at risk for early-onset thrombocytopenia upon treatment with GPIIb/IIIa antagonist/agonists, thereby increasing the specificity of antibody detection. This invention also relates to the use of DDABs as a positive control and calibration standard for DDAB assays. Such methods, procedures and assays are useful for identifying patients who may be at risk to develop disease states mediated by treatment with integrin antagonists/agonists.

摘要翻译： 本发明涉及检测发展为整合素拮抗剂/激动剂介导的疾病状态的风险的患者。 本发明涉及可用于在整联蛋白拮抗剂/激动剂存在下结合整联蛋白或整合蛋白相关蛋白或其复合物的药物依赖性抗体的患者体液样品中的检测。 本发明还涉及用于在整联蛋白激动剂存在下结合整联蛋白（包括血小板糖蛋白IIb / IIIa（GPIIb / IIIa））的药物依赖性抗体（DDABS）的患者体液样品中检测的测定法， /或拮抗剂。 本发明还涉及识别不易引起整联蛋白拮抗剂/激动剂介导的疾病状态的整联蛋白拮抗剂/激动剂的方法。本发明还涉及增加体液中整联蛋白定向抗体的回收率的方法，导致敏感性增加 和DDAB检测测定的特异性。 本发明还涉及用于治疗血液样品的方法，其从血小板表面解离GPIIb / IIIa的抗体，从而增加血小板上清液的回收。 本发明还涉及使用不同的GPIIb / IIIa制剂来鉴定在用GPIIb / IIIa拮抗剂/激动剂治疗时患有早发性血小板减少症的患者，从而增加抗体检测的特异性。本发明还涉及使用DDAB 作为DDAB测定的阳性对照和校准标准。这些方法，程序和测定法可用于鉴定可能患有通过用整联蛋白拮抗剂/激动剂治疗介导的疾病状态的风险的患者。

公开(公告)号：US08980583B2

公开(公告)日：2015-03-17

申请号：US13538825

申请日：2012-06-29

申请人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

发明人： Nan Lin ,  Henry J. George ,  Joaquina Mascarenhas ,  Trevor N. Collingwood ,  Kevin J. Kayser ,  Katherine Achtien

IPC分类号： C12N5/10 ,  C12N9/02 ,  C12P21/00 ,  C12N9/10

CPC分类号： C12P21/005 ,  C12N9/0071 ,  C12N9/1051 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2510/04 ,  C12Y114/18002

摘要： The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.

摘要翻译： 本发明提供缺乏CMP-Neu5Ac羟化酶（Cmah）和/或糖蛋白α-1,3-半乳糖基转移酶（Ggta1）的非人哺乳动物细胞系。 还提供了使用本文公开的细胞来生产具有人类糖基化模式的重组蛋白质的方法。