公开(公告)号：US5252493A

公开(公告)日：1993-10-12

申请号：US221248

申请日：1988-07-22

申请人： Koichi Fujiwara ,  Juichi Noda ,  Hiromichi Mizutani ,  Hiroko Mizutani

发明人： Koichi Fujiwara ,  Juichi Noda ,  Hiromichi Mizutani ,  Hiroko Mizutani

IPC分类号： G01N21/51 ,  G01N33/543 ,  G01N33/58 ,  G01N35/00 ,  G01N35/10 ,  G01N33/553

CPC分类号： G01N21/51 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/585 ,  G01N2035/1025 ,  G01N35/0098

摘要： An ultra-sensitive laser magnetic immunoassay method comprising the steps of: labeling an antigen or antibody with micro-particles of a magnetic substance to form a magnetic-labeled body; subjecting a specimen and the magnetic-labeled body to an antigen-antibody reaction to form a reacted body-specimen complex; separating and removing unreacted body from the reacted complex; guiding and concentrating the reacted complex magnetically; irradiating the concentrated complex with a laser beam; detecting outgoing light from a measurement system to provide a quantitative result in the picogram range. An apparatus comprising a specimen container for storing a specimen labeled with magnetic micro-particles, concentration means for guiding and locally concentrating the specimen, a laser beam irradiating optical system for guiding a laser beam into the specimen, and a light receiving system arranged so as to detect outgoing beams from the specimen.

摘要翻译： PCT No.PCT / JP87 / 00694 Sec。 371日期：1988年7月22日 102（e）日期1988年7月22日PCT提交1987年9月22日PCT公布。 出版物WO88 / 02118 日本3月24日，1988年。一种超灵敏的激光磁性免疫测定方法，其特征在于，包括以下步骤：利用磁性物质的微粒子标记抗原或抗体，形成磁性标记体; 将标本和磁性标记物进行抗原 - 抗体反应，形成反应体样品复合体; 从反应的络合物中分离和除去未反应的物质; 引导和浓缩反应复合物磁性; 用激光束照射浓缩络合物; 检测来自测量系统的出射光，以提供皮克范围的定量结果。 一种装置，包括：用于存储用磁性微粒子标记的标本的检体容器，用于引导和局部集中样本的浓缩装置，用于将激光束引导到样本中的激光束照射光学系统;以及光接收系统， 以检测来自样品的出射光束。

公开(公告)号：US5238811A

公开(公告)日：1993-08-24

申请号：US875529

申请日：1992-04-27

申请人： Koichi Fujiwara ,  Hiroko Mizutani ,  Hiromichi Mizutani, deceased ,  Shuichi Shibata ,  Koichi Arishima ,  Mitsutoshi Hoshino ,  Yasushi Hoshino

发明人： Koichi Fujiwara ,  Hiroko Mizutani ,  Hiromichi Mizutani, deceased ,  Shuichi Shibata ,  Koichi Arishima ,  Mitsutoshi Hoshino ,  Yasushi Hoshino

IPC分类号： C12Q1/70 ,  G01N33/553

CPC分类号： G01N27/745 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/813 ,  Y10S436/817

摘要： A laser magnetic immunoassay (LMIA) technique is presented which combines the high detection sensitivity of the radioimmunoassay (RIA) technique with the simplicity of particle agglutination (PA) technique. The LMIA technique dispenses with the separation step of bound and free species (B/F separation), yet provides detectivity of the order of picogram of viral species per milliliter of analyte solution. The procedure includes of the steps of: preparing a superparamagnetic-labeled body of antigen or antibody; subjecting a specimen sample and the magnetic-labeled body to a specific immunoreaction to produce a reacted body; subjecting the reacted body containing both bound and free species to a spot magnetic field gradient; irradiating the spot with a laser beam; measuring the intensity of the outgoing light from the spot; and making a quantitative determination of the virus according to the time difference in the outgoing light signals generated by bound and free species.

摘要翻译： 提出了一种将放射免疫测定（RIA）技术的高检测灵敏度与粒子凝集（PA）技术的简单结合在一起的激光磁免疫测定（LMIA）技术。 LMIA技术免除了结合和游离物质（B / F分离）的分离步骤，但是每毫升分析物溶液提供了病毒物质的皮克级数的检测率。 该方法包括以下步骤：制备抗原或抗体的超顺磁标记体; 对标本样品和磁性标记物进行特异性免疫反应以产生反应体; 使含有结合物和自由物质的反应体经受斑点磁场梯度; 用激光束照射点; 测量来自现场的出射光的强度; 并根据绑定和自由物种产生的出射光信号的时差进行病毒的定量测定。

公开(公告)号：US5236824A

公开(公告)日：1993-08-17

申请号：US812132

申请日：1991-12-18

申请人： Koichi Fujiwara ,  Hiroko Mizutani ,  Hiromichi Mizutani, deceased ,  Shuichi Shibata ,  Koichi Arishima ,  Mitsutoshi Hoshino ,  Yasushi Hoshino

发明人： Koichi Fujiwara ,  Hiroko Mizutani ,  Hiromichi Mizutani, deceased ,  Shuichi Shibata ,  Koichi Arishima ,  Mitsutoshi Hoshino ,  Yasushi Hoshino

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N35/00

CPC分类号： G01N33/585 ,  G01N33/54326 ,  G01N35/0098 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/813 ,  Y10S436/817

摘要： An in-situ laser magnetic immunoassay ("LMIA") method which eliminates the step of B/F separation generally required in the labeling method of immunoassays. The laser magnetic immunoassay permits a quantitative determination of a target immunological substance, for example, an antigen, an antibody, lymphocytes, viruses, tumorous cells and infections cells, in an analyte solution containing both bound and free species. A transitory increase in the magnetophoretic scattering of laser beam is observed when the analyte solution contains magnetic-labeled, bound target analyte, while no such increase is observed in a control test solution, containing only the relevant reagents. A magnetophoretic LMIA apparatus is provided which includes a magnetic gradient generating device which forms an integral part of the in-situ LMIA.

摘要翻译： 原位激光免疫测定（“LMIA”）方法消除了免疫测定的标记方法通常需要的B / F分离步骤。 激光磁性免疫测定允许在含有结合和游离物种的分析物溶液中定量测定靶免疫学物质，例如抗原，抗体，淋巴细胞，病毒，肿瘤细胞和感染细胞。 当分析物溶液含有磁性标记的结合目标分析物时，观察到激光束的磁电泳散射的短暂增加，而在仅包含相关试剂的对照试验溶液中没有观察到这种增加。 提供了一种磁电泳LMIA装置，其包括形成原位LMIA的组成部分的磁梯度发生装置。