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    NUCLEIC ACID FOR USE IN ALGAE AND USE THEREOF
    1.
    发明申请
    NUCLEIC ACID FOR USE IN ALGAE AND USE THEREOF 审中-公开
    核酸用于阿尔及其使用

    公开(公告)号：US20110014708A1

    公开(公告)日：2011-01-20

    申请号：US12505210

    申请日：2009-07-17

    申请人： Huai-Jen Tsai ,  Si-Shen Li

    发明人： Huai-Jen Tsai ,  Si-Shen Li

    IPC分类号： C12N1/13 ,  C07H21/04 ,  C12N15/09

    CPC分类号： C07K14/79 ,  C12N1/12 ,  C12N15/8257

    摘要： The present invention provides a modified nucleic acid for expressing bovine lactoferricin (LFB) in algae, which consists of SEQ ID NO: 3. The present invention also provides a method of producing a foreign desired gene product in algae, comprising: (a) weakening or removing cell wall of the algae by a protein enzyme solution to make algae become suitable for gene transfer; (b) transferring a foreign gene encoding the desired gene product into the algae; and (c) expressing the foreign gene to produce the desired gene product. The present invention further provides a feed composition comprising a transgenic algae or its offspring, wherein the cell wall of said transgenic algae is weakened or removed by a protein enzyme solution.

    摘要翻译： 本发明提供了用于在藻类中表达牛乳铁蛋白（LFB）的修饰的核酸，其由SEQ ID NO：3组成。本发明还提供了一种在藻类中制备外来期望的基因产物的方法，包括：（a）弱化 或通过蛋白酶溶液去除藻类的细胞壁，使藻类变得适合于基因转移; （b）将编码所需基因产物的外源基因转移到藻类中; 和（c）表达外源基因以产生所需的基因产物。 本发明还提供了包含转基因藻类或其后代的饲料组合物，其中所述转基因藻类的细胞壁被蛋白酶溶液削弱或去除。

    Method for producing heart-specific fluorescence of non-human eukaryotic animals
    2.
    发明申请
    Method for producing heart-specific fluorescence of non-human eukaryotic animals 有权
    生产非人类真核动物心脏特异性荧光的方法

    公开(公告)号：US20050273874A1

    公开(公告)日：2005-12-08

    申请号：US11017814

    申请日：2004-12-22

    申请人： Huai-Jen Tsai ,  Chiu-Ju Huang ,  Chung-Der Hsiao

    发明人： Huai-Jen Tsai ,  Chiu-Ju Huang ,  Chung-Der Hsiao

    IPC分类号： A01K67/00 ,  A01K67/027 ,  C12N15/85

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/0375 ,  C12N2830/008

    摘要： A method of expressing in vivo heart-specific fluorescence in transgenic line of zebrafish is developed, which provides a research model for studying heart-related gene functions and performing gene therapies in the future. The method comprises the following steps. Firstly, a plasmid is constructed. This plasmid construct includes the upstream regulatory region, the exon 1, the intron 1, and the exon 2 of cmlc2 gene, cDNA of GFP, wherein the cmlc2 gene and GFP cDNA form a cassette, and inverted terminal repeats from adeno-associated virus are flanked at both sides of this cassette. The plasmid construct is linearized and microinjected into one-celled zebrafish fertilized eggs. Lastly, the heart-specific fluorescent expressed zebrafish are selected and the germline-transmitting transgenic strain is generated.

    摘要翻译： 开发了在斑马鱼转基因系中体内表达体内特异性荧光的方法，为研究心脏相关基因功能和未来基因治疗提供了研究依据。 该方法包括以下步骤。 首先构建质粒。 该质粒构建体包括上游调节区，cmlc2基因的外显子1，内含子1和外显子2，GFP的cDNA，其中cmlc2基因和GFP cDNA形成盒，来自腺相关病毒的反向末端重复 侧面在这个盒子的两侧。 将质粒构建体线性化并显微注射到单细胞斑马鱼受精卵中。 最后，选择心脏特异性荧光表达的斑马鱼，并产生种系传播转基因株。

    Methods for producing transgenic medaka
    4.
    发明申请
    Methods for producing transgenic medaka 审中-公开
    生产转基因药物的方法

    公开(公告)号：US20060162005A1

    公开(公告)日：2006-07-20

    申请号：US11377838

    申请日：2006-03-17

    申请人： Huai-Jen Tsai

    发明人： Huai-Jen Tsai

    IPC分类号： A01K67/027

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/43595

    摘要： The invention relates to a recombinant DNA method for producing transgenic medaka. The invention also relates to novel gene fragments for producing the transgenic medaka. The invention further relates to novel transgenic medaka.

    摘要翻译： 本发明涉及用于产生转基因药物的重组DNA方法。 本发明还涉及用于产生转基因med to的新基因片段。 本发明还涉及新型转基因药物。

    Method for producing heart-specific fluorescence of non-human eukaryotic animals
    7.
    发明授权
    Method for producing heart-specific fluorescence of non-human eukaryotic animals 有权
    生产非人类真核动物心脏特异性荧光的方法

    公开(公告)号：US07355095B2

    公开(公告)日：2008-04-08

    申请号：US11017814

    申请日：2004-12-22

    申请人： Huai-Jen Tsai ,  Chiu-Ju Huang ,  Chung-Der Hsiao

    发明人： Huai-Jen Tsai ,  Chiu-Ju Huang ,  Chung-Der Hsiao

    IPC分类号： C12N15/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/0375 ,  C12N2830/008

    摘要： A method of expressing in vivo heart-specific fluorescence in transgenic line of zebrafish is developed, which provides a research model for studying heart-related gene functions and performing gene therapies in the future. The method comprises the following steps. Firstly, a plasmid is constructed. This plasmid construct includes the upstream regulatory region, the exon 1, the intron 1, and the exon 2 of cmlc2 gene, cDNA of GFP, wherein the cmlc2 gene and GFP cDNA form a cassette, and inverted terminal repeats from adeno-associated virus are flanked at both sides of this cassette. The plasmid construct is linearized and microinjected into one-celled zebrafish fertilized eggs. Lastly, the heart-specific fluorescent expressed zebrafish are selected and the germline-transmitting transgenic strain is generated.

    摘要翻译： 开发了在斑马鱼转基因系中体内表达体内特异性荧光的方法，为研究心脏相关基因功能和未来基因治疗提供了研究依据。 该方法包括以下步骤。 首先构建质粒。 该质粒构建体包括上游调节区，cmlc2基因的外显子1，内含子1和外显子2，GFP的cDNA，其中cmlc2基因和GFP cDNA形成盒，来自腺相关病毒的反向末端重复 侧面在这个盒子的两侧。 将质粒构建体线性化并显微注射到单细胞斑马鱼受精卵中。 最后，选择心脏特异性荧光表达的斑马鱼，并产生种系传播转基因株。

    METHOD FOR ENHANCING PRODUCTION OF PATHOGEN-RESISTANT PROTEINS USING BIOREACTOR
    8.
    发明申请
    METHOD FOR ENHANCING PRODUCTION OF PATHOGEN-RESISTANT PROTEINS USING BIOREACTOR 审中-公开
    使用生物反应器增强抗病原蛋白生产的方法

    公开(公告)号：US20120054880A1

    公开(公告)日：2012-03-01

    申请号：US13216396

    申请日：2011-08-24

    申请人： HUAI-JEN TSAI ,  CHENG-YUNG LIN

    发明人： HUAI-JEN TSAI ,  CHENG-YUNG LIN

    IPC分类号： C12N15/63

    CPC分类号： C12N15/67 ,  A01K67/0275 ,  A01K2217/00 ,  A01K2227/40 ,  A01K2267/01

    摘要： A method for enhancing production of pathogen-resistant proteins using bioreactor comprising: (a) constructing a plasmid which comprises a nucleic acid sequence of exogenous pathogen-resistant protein, a nucleic acid sequence of fluorescence protein, a nucleic acid sequence of protease-cutting site and an α-actin promoter; (b) transferring the constructed plasmid into fish embryo(s) of selected line after linearization; (c) domesticating the fish to enable to grow, mate and spawn; and (d) collecting fish egg(s), fish embryo(s) and adult fish(s) to obtain pathogen-resistant protein-rich organism(s) is described. A nucleic acid construct comprising an α-actin promoter, a nucleic acid sequence of exogenous pathogen-resistant protein and a nucleic acid sequence of fluorescence protein which are operably linked in the 5′ to 3′ direction is also presented. An expression vector comprising said nucleic acid construct is further described.

    摘要翻译： 一种使用生物反应器增强病原体抗性蛋白质生产的方法，包括：（a）构建质粒，其包含外源性病原体抗性蛋白质的核酸序列，荧光蛋白的核酸序列，蛋白酶切割位点的核酸序列 和α-肌动蛋白启动子; （b）线性化后将构建的质粒转移到选定品系的鱼胚中; （c）驯养鱼以使其生长，交配和产卵; 描述了（d）收集鱼卵，鱼胚和成年鱼以获得富含病原体的富含蛋白质的生物体。 还提出了包含α-肌动蛋白启动子，外源性病原体抗性蛋白的核酸序列和荧光蛋白的核酸序列的核酸构建体，其在5'至3'方向上可操作地连接。 进一步描述了包含所述核酸构建体的表达载体。

    Recombinant plasmid expressing two fluorescence genes
    10.
    发明授权
    Recombinant plasmid expressing two fluorescence genes 失效
    表达两种荧光基因的重组质粒

    公开(公告)号：US07365177B2

    公开(公告)日：2008-04-29

    申请号：US10759268

    申请日：2004-01-20

    申请人： Huai-Jen Tsai

    发明人： Huai-Jen Tsai

    IPC分类号： C07H21/02 ,  C07H21/04 ,  A01K67/027

    CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/43595 ,  C12N15/8509 ,  C12N2830/008

    摘要： Recombinant plasmids comprising (a) a ubiquitous promoter, (b) one fluorescent gene, the gene being operably liked to and inserted downstream of the ubiquitous promoter, (c) a skin-specific or muscle-specific promoter, and (d) another fluorescent gene, the gene being operably linked to and inserted downstream the skin-specific or muscle specific promoter. The ubiquitous promoter and the skin-specific or muscle promoter have the adverse directional property and the ubiquitous promoter and the skin-specific or muscle-specific promoter are located upstream of the fluorescent gene and the another fluorescent gene respectively so as to have the directional property which permits transcription of the genes. Host cells, transgenic fish harboring the plasmid of the invention and methods of producing a transgenic fish can be made with the plasmids of the invention.

    摘要翻译： 重组质粒，其包含（a）普遍存在的启动子，（b）一个荧光基因，该基因可操作地喜欢并插入普遍存在的启动子的下游，（c）皮肤特异性或肌肉特异性启动子，和（d）另一种荧光 基因，该基因与皮肤特异性或肌肉特异性启动子可操作地连接并插入下游。 无处不在的启动子和皮肤特异性或肌肉启动子具有不利的方向性质，普遍存在的启动子和皮肤特异性或肌肉特异性启动子分别位于荧光基因和另一荧光基因的上游，以具有定向性 这允许基因的转录。 宿主细胞，携带本发明质粒的转基因鱼和生产转基因鱼的方法可用本发明的质粒制备。