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1.发明授权Process for preparing L-amino acids with corynebacteria with enhanced nucleotide sequences coding for pknD 有权用增强的编码pknD的核苷酸序列制备具有棒状杆菌的L-氨基酸的方法公开(公告)号：US07226763B2
公开(公告)日：2007-06-05
申请号：US11110837
申请日：2005-04-21
申请人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
发明人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
CPC分类号： C12N9/1205 , C12R1/15
摘要： An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译： 一种分离的多核苷酸，其含有选自以下的多核苷酸序列：（a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％的多核苷酸，（b） 编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，（c）与（a）或（b）的多核苷酸互补的多核苷酸和（ d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状杆菌制备L-氨基酸的发酵方法，其中至少pknD基因是 扩增和使用含有本发明序列的多核苷酸作为杂交探针。
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公开(公告)号：US20050255566A1
公开(公告)日：2005-11-17
申请号：US11110837
申请日：2005-04-21
申请人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
发明人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
CPC分类号： C12N9/1205 , C12R1/15
摘要： An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译： 一种分离的多核苷酸，其含有选自以下的多核苷酸序列：（a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％的多核苷酸，（b） 编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，（c）与（a）或（b）的多核苷酸互补的多核苷酸和（ d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状杆菌制备L-氨基酸的发酵方法，其中至少pknD基因是 扩增和使用含有本发明序列的多核苷酸作为杂交探针。
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公开(公告)号：US06927052B2
公开(公告)日：2005-08-09
申请号：US09949971
申请日：2001-09-12
申请人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
发明人： Brigitte Bathe , Indra Schroeder , Mike Farwick , Thomas Hermann
CPC分类号： C12N9/1205 , C12R1/15
摘要： An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
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