摘要:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译:一种分离的多核苷酸,其含有选自以下的多核苷酸序列:(a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%的多核苷酸,(b) 编码含有与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列的多肽,(c)与(a)或(b)的多核苷酸互补的多核苷酸和( d)包含(a),(b)或(c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状杆菌制备L-氨基酸的发酵方法,其中至少pknD基因是 扩增和使用含有本发明序列的多核苷酸作为杂交探针。
摘要:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译:一种分离的多核苷酸,其含有选自以下的多核苷酸序列:(a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%的多核苷酸,(b) 编码含有与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列的多肽,(c)与(a)或(b)的多核苷酸互补的多核苷酸和( d)包含(a),(b)或(c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状杆菌制备L-氨基酸的发酵方法,其中至少pknD基因是 扩增和使用含有本发明序列的多核苷酸作为杂交探针。
摘要:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要:
A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigC gene from Corynebacterium glutamicum, and a host-vector system having a coryneform host bacterium, such as Corynebacterium glutamicum, and a vector which carries at least the sigC gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码来自谷氨酸棒杆菌的sigC基因的多核苷酸序列的分离的多核苷酸,以及具有棒状杆菌宿主细菌如谷氨酸棒杆菌的宿主 - 载体系统,以及至少携带至少sigC基因的载体 SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to a process for the production of L-amino acids, in which the following steps are carried out: a) fermentation of the coryneform bacteria producing the desired L-amino acid, in which at least the mqo gene is attenuated, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the L-amino acid, and, optionally, bacteria are used in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally enhanced, or bacteria are used in which at least some of the metabolic pathways reducing formation of the desired L-amino acid are excluded.
摘要:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要翻译:一种分离的多核苷酸,其含有选自以下的多核苷酸序列:(a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%的多核苷酸,(b) 编码含有与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列的多肽,(c)与(a)或(b)的多核苷酸互补的多核苷酸和( d)包含(a),(b)或(c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用其中至少pknB基因被扩增的棒状杆菌制备L-氨基酸的发酵方法 以及使用含有本发明序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码σ因子M(sigM)基因的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸,以及具有其中sigM基因以增强形式存在的棒状细胞宿主细菌的宿主 - 载体系统和载体, 至少携带根据SEQ ID No 1的sigM基因,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep34 gene, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.