公开(公告)号：US09422568B2

公开(公告)日：2016-08-23

申请号：US14059900

申请日：2013-10-22

Applicant: Nadja Jessberger ,  Andreas Burkovski ,  Brigitte Bathe ,  Alexander Reth

Inventor： Nadja Jessberger ,  Andreas Burkovski ,  Brigitte Bathe ,  Alexander Reth

IPC: C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N15/77 ,  C07K14/34 ,  C12P13/04 ,  C12P13/08

CPC classification number: C12N15/77 ,  C07K14/34 ,  C12P13/04 ,  C12P13/08

Abstract: A process for preparing L-amino acids employing coryneform bacteria in which the AmtR regulator has been attenuated is provided. Recombinant bacteria, polynucleotides and vectors corresponding to or having the attenuated AmtR regulator are disclosed.

Abstract translation: 提供了使用其中AmtR调节剂已被减毒的棒状细菌制备L-氨基酸的方法。 公开了对应于或具有衰减的AmtR调节剂的重组细菌，多核苷酸和载体。

公开(公告)号：US09045762B2

公开(公告)日：2015-06-02

申请号：US14532259

申请日：2014-11-04

Applicant: Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

Inventor： Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

IPC: C12P13/08 ,  C12N15/77 ,  C12P13/10 ,  C12P13/06

CPC classification number: C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10

Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.

公开(公告)号：US08592187B2

公开(公告)日：2013-11-26

申请号：US13027732

申请日：2011-02-15

Applicant: Brigitte Bathe ,  Wilfried Claes ,  Silke Jerrentrup ,  Caroline Kreutzer ,  Georg Thierbach

Inventor： Brigitte Bathe ,  Wilfried Claes ,  Silke Jerrentrup ,  Caroline Kreutzer ,  Georg Thierbach

IPC: C12P13/04 ,  C12P13/22 ,  C12P13/08 ,  C12P13/06

CPC classification number: C12N15/77 ,  A23K10/12 ,  A23K20/147 ,  C07K14/34 ,  C12P13/04 ,  C12P13/08 ,  C12P13/227

Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.

Abstract translation: 本发明涉及编码OxyR转录调节子变体的棒状细菌的oxyR基因的突变体和等位基因，以及使用包含这些等位基因的细菌产生氨基酸的方法。

公开(公告)号：US20120190084A1

公开(公告)日：2012-07-26

申请号：US13353426

申请日：2012-01-19

Applicant: Frank Schneider ,  Stella Molck ,  Brigitte Bathe

Inventor： Frank Schneider ,  Stella Molck ,  Brigitte Bathe

IPC: C12P13/12 ,  C12N1/21

CPC classification number: C12P13/12 ,  C12N9/13 ,  C12R1/15 ,  C12Y208/01001 ,  Y02P20/52

Abstract: Disclosed herein is a process for the fermentative preparation of sulphur-containing amino acids chosen from the group of L-methionine, L-cysteine, L-cystine, L-homocysteine and L-homocystine, comprising the steps: a) provision of a microorganism of the family Enterobacteriaceae or of a microorganism of the family Corynebacteriaceae which has an increased thiosulphate sulphurtransferase activity compared with the particular starting strain; b) fermentation of the microorganism from a) in a medium which contains an inorganic source of sulphur chosen from the group of salt of dithiosulphuric acid or a mixture of a salt of dithiosulphuric acid and a salt of sulphuric acid, a fermentation broth being obtained, and c) concentration of the sulphur-containing amino acid in the fermentation broth from b).

Abstract translation: 本文公开了一种选自L-甲硫氨酸，L-半胱氨酸，L-胱氨酸，L-高半胱氨酸和L-高胱氨酸的含硫氨基酸发酵制备方法，包括以下步骤：a）提供微生物 的家族肠杆菌科或科氏杆菌科的微生物，其与特定的起始菌株相比具有增加的硫代硫酸盐转移酶活性; b）从含有选自二硫代硫酸盐或二硫代硫酸盐与硫酸盐的混合物的硫的无机硫源的培养基中培养微生物，发酵培养基， 和c）来自b）的发酵液中的含硫氨基酸的浓度。

公开(公告)号：US20120164698A1

公开(公告)日：2012-06-28

申请号：US13379654

申请日：2010-06-18

Applicant: Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

Inventor： Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

IPC: C12P13/04 ,  C12P13/12 ,  C12N1/21 ,  C12P13/22 ,  C12N15/54 ,  C12P13/08 ,  C12P13/06

CPC classification number: C12P13/08 ,  C12N9/1205 ,  C12Y207/01023

Abstract: The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.

Abstract translation: 本发明涉及一种制备有机化合物的方法，其特征在于进行以下步骤：a）分泌L-氨基酸的微生物的发酵，该微生物含有编码具有多磷酸依赖性多肽的过表达多核苷酸 NAD +激酶活性，在发酵培养基中形成发酵液，b）所述化合物在所述发酵液中和/或所述微生物细胞中的积累。 本发明涉及通过发酵其中具有多磷酸依赖性NAD +激酶的多肽被过表达的微生物来制备有机化合物的方法。

公开(公告)号：US20110318791A1

公开(公告)日：2011-12-29

申请号：US13118645

申请日：2011-05-31

Applicant: Stephan HANS ,  Brigitte Bathe ,  Georg Thierbach

Inventor： Stephan HANS ,  Brigitte Bathe ,  Georg Thierbach

IPC: C12P13/24

CPC classification number: C12N9/12

Abstract: The invention relates to processes for fermentative production of L-proline using bacteria which contain mutated variants of the proB gene.

Abstract translation: 本发明涉及使用含有proB基因突变变体的细菌发酵生产L-脯氨酸的方法。

公开(公告)号：US07915394B2

公开(公告)日：2011-03-29

申请号：US12696449

申请日：2010-01-29

Applicant: Natalie Schischka ,  Brigitte Bathe ,  Georg Thierbach

Inventor： Natalie Schischka ,  Brigitte Bathe ,  Georg Thierbach

IPC: C12P1/00 ,  C12P13/04 ,  C07H21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/20 ,  C12N1/21

CPC classification number: C12P13/08 ,  C12N9/0006 ,  C12P13/227

Abstract: The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.

Abstract translation: 本发明涉及编码葡萄糖-6-磷酸脱氢酶（EC：1.1.1.49）的OpcA亚基变体的棒状杆菌细菌的opcA基因的突变体和等位基因，以及制备氨基酸，特别是L-赖氨酸和 L-色氨酸通过使用含有所述等位基因的细菌。

公开(公告)号：US20100255544A1

公开(公告)日：2010-10-07

申请号：US12553647

申请日：2009-09-03

Applicant: Brigitte Bathe ,  Caroline Kreutzer ,  Bettina Mockel ,  Georg Thierbach

Inventor： Brigitte Bathe ,  Caroline Kreutzer ,  Bettina Mockel ,  Georg Thierbach

IPC: C12P19/38 ,  C12P1/00 ,  C12P19/30 ,  C12P13/04 ,  C12P13/24 ,  C12P13/22 ,  C12P13/20 ,  C12P13/14 ,  C12P13/12 ,  C12P13/08 ,  C12P13/06 ,  C12N15/74 ,  C12N1/21 ,  C12N15/63

CPC classification number: C12P13/08

Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.

Abstract translation: 本发明涉及棒状细菌，代替开放阅读框（ORF）的单一拷贝，天然存在于特定所需位点（基因座）的基因或等位基因具有至少两个开放阅读框（ORF）的拷贝， 基因或等位基因，优选串联排列，以及任选地在另一基因位点处的开放阅读框（ORF），所述基因或等位基因的至少第三拷贝，以及通过这些发酵制备化合物的方法 菌。

公开(公告)号：US20100159523A1

公开(公告)日：2010-06-24

申请号：US12565533

申请日：2009-09-23

Applicant: BRIGITTE BATHE ,  CAROLINE KREUTZER ,  BETTINA MOCKEL ,  GEORG THIERBACH

Inventor： BRIGITTE BATHE ,  CAROLINE KREUTZER ,  BETTINA MOCKEL ,  GEORG THIERBACH

IPC: C12P19/38 ,  C12P19/30 ,  C12P13/04 ,  C12P13/24 ,  C12P13/22 ,  C12P13/20 ,  C12P13/14 ,  C12P13/12 ,  C12P13/10 ,  C12P13/08 ,  C12P13/06 ,  C12N15/74 ,  C12N1/20 ,  C12N15/63

CPC classification number: C12R1/15 ,  C12N15/52 ,  C12P13/08

Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.

Abstract translation: 本发明涉及除了至少一个拷贝存在于编码合成蛋白质或RNA的开放阅读框（ORF），基因或等位基因的天然位点（基因座）外的棒状细菌， 在每种情况下，该开放阅读框（ORF）的第二个，任选的第三或第四个拷贝，基因或等位基因在每种情况下都是整合到染色体中的形式的第二个，任选的第三或第四个位点，并且用于制备化合物 通过这些细菌的发酵。

公开(公告)号：US07618798B2

公开(公告)日：2009-11-17

申请号：US11705714

申请日：2007-02-14

Applicant: Brigitte Bathe ,  Stephan Hans ,  Natalie Schischka ,  Georg Thierbach

Inventor： Brigitte Bathe ,  Stephan Hans ,  Natalie Schischka ,  Georg Thierbach

IPC: C12P13/04 ,  C12P13/22

CPC classification number: C12P13/04 ,  C12N9/0006

Abstract: The invention relates to mutants and alleles of the coryneform bacterium mqo gene which encodes malate quinone oxidoreductases which contain any amino acid apart from L-serine at position 111, or a comparable position, in the amino acid sequence, and to processes for fermentatively preparing amino acids, preferably L-lysine, L-tryptophan and L-proline, using bacteria which comprise these alleles.

Abstract translation: 本发明涉及棒状细菌mqo基因的突变体和等位基因，其编码苹果酸醌氧化还原酶，其在氨基酸序列中含有111位的L-丝氨酸或氨基酸序列的相当位置的任何氨基酸，以及用于发酵制备氨基酸的方法 酸，优选L-赖氨酸，L-色氨酸和L-脯氨酸，使用包含这些等位基因的细菌。