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    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants
    2.
    发明授权
    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants 有权
    具有在植物中起作用的真菌3'终止序列的重组表达盒

    公开(公告)号：US07910717B2

    公开(公告)日：2011-03-22

    申请号：US11962357

    申请日：2007-12-21

    申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8216

    摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element. and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

    摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包括可操作地连接到具有终止密码子的编码序列和真菌终止序列的植物启动子。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件。 和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。

    Recombinant expression cassettes with a fungal 3' termination sequence that function in plants
    3.
    发明授权
    Recombinant expression cassettes with a fungal 3' termination sequence that function in plants 有权
    具有在植物中起作用的真菌3'终止序列的重组表达盒

    公开(公告)号：US07893239B2

    公开(公告)日：2011-02-22

    申请号：US11962414

    申请日：2007-12-21

    申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8216

    摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

    摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有终止密码子的编码序列和真菌终止序列的植物启动子。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。

    Plastid transit peptides
    4.
    发明授权
    Plastid transit peptides 失效
    质体转运肽

    公开(公告)号：US07557260B2

    公开(公告)日：2009-07-07

    申请号：US12015342

    申请日：2008-01-16

    申请人： Michael Lassner ,  Jack Q. Wilkinson

    发明人： Michael Lassner ,  Jack Q. Wilkinson

    IPC分类号： C12N15/09 ,  C12N15/82 ,  A01H5/00 ,  C07H21/00

    CPC分类号： C07K14/415 ,  C07K14/00 ,  C12N15/8221

    摘要： The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

    摘要翻译： 本发明提供了将附着部分（例如，多肽）定位于植物质体中的新型质体转运肽。 本发明还涉及用于将多肽定位于植物质体的方法和组合物，包括但不限于转基因植物生产。

    Regulatory element for heterologous protein production in the fruiting body of filamentous fungi
    6.
    发明授权
    Regulatory element for heterologous protein production in the fruiting body of filamentous fungi 有权
    丝状真菌子实体异源蛋白质生产的调控因子

    公开(公告)号：US09145561B2

    公开(公告)日：2015-09-29

    申请号：US13819356

    申请日：2011-08-30

    申请人： Zhixin Shi ,  Jack Q. Wilkinson ,  Donald S. Walters ,  C. Peter Romaine

    发明人： Zhixin Shi ,  Jack Q. Wilkinson ,  Donald S. Walters ,  C. Peter Romaine

    IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N1/15 ,  C12N15/81 ,  C12N15/80 ,  C12N15/113 ,  C07K14/81 ,  C07K14/37

    CPC分类号： C12N15/80 ,  C07K14/37 ,  C07K14/8117 ,  C12N15/113

    摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

    摘要翻译： 本发明提供调节真菌中核苷酸序列表达的组合物和方法。 组合物是从双孢蘑菇凝集素基因分离的组织优选启动子的新核苷酸序列。 序列优先驱动表达水果体组织。 提供了使用本文公开的调节序列在真菌中表达核苷酸序列的方法。 该方法包括转化真菌细胞以包含可操作地连接到本发明的一个或多个调节序列的核苷酸序列，并从转化的细胞再生稳定转化的真菌。

    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants
    7.
    发明授权
    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants 有权
    具有在植物中起作用的真菌3'终止序列的重组表达盒

    公开(公告)号：US07851611B2

    公开(公告)日：2010-12-14

    申请号：US11959752

    申请日：2007-12-19

    申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8216

    摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

    摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有终止密码子的编码序列和真菌终止序列的植物启动子。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。

    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants
    10.
    发明授权
    Recombinant expression cassettes with a fungal 3′ termination sequence that function in plants 有权
    具有在植物中起作用的真菌3'终止序列的重组表达盒

    公开(公告)号：US07851613B2

    公开(公告)日：2010-12-14

    申请号：US11960919

    申请日：2007-12-20

    申请人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    发明人： Jack Q. Wilkinson ,  Kevin McBride ,  Sean Bertain

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8216

    摘要： The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop condon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

    摘要翻译： 本发明提供了包含在植物中有功能的真菌3'终止序列的重组表达盒。 重组表达盒包含可操作地连接到具有停止螺旋的编码序列的植物启动子和真菌终止序列。 真菌3'终止序列与编码序列是异源的。 真菌3'终止序列包括结构特征，包括切割位点，定位元件和上游元件。 本发明还包括用于构建植物表达盒并将盒引入植物细胞的方法。