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    Methods and Apparatus for Amplifying Nucleic Acids
    2.
    发明申请
    Methods and Apparatus for Amplifying Nucleic Acids 审中-公开
    用于扩增核酸的方法和装置

    公开(公告)号：US20160046977A1

    公开(公告)日：2016-02-18

    申请号：US14738179

    申请日：2015-06-12

    申请人： Nihan Aydemir ,  Jadranka Travas-Sejdic ,  Clive William Evans ,  David Edward Williams

    发明人： Nihan Aydemir ,  Jadranka Travas-Sejdic ,  Clive William Evans ,  David Edward Williams

    IPC分类号： C12Q1/68 ,  G01N27/02

    CPC分类号： C12Q1/686 ,  G01N27/02 ,  G01N27/026 ,  C12Q2561/113 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/537 ,  C12Q2565/607

    摘要： The present invention relates to methods and apparatuses for amplifying, detecting, and optionally quantifying, nucleic acids. In one aspect the method comprises (a) providing a reaction volume comprising (i) a first electrode comprising an electrochemically-active conducting polymer, a first single-stranded nucleic acid molecule capable of hybridizing to a target nucleic acid, wherein the first nucleic acid molecule is covalently attached to the electrochemically-active conducting polymer, and (ii) a second electrode, (b) providing a reaction mixture to the reaction volume, the reaction mixture comprising a target nucleic acid, a nucleic acid polymerase, a redox couple, and nucleic acid amplification reagents, (c) amplifying the nucleic acid, and (d) measuring the impedance of the first electrode at least once during the nucleic acid amplification reaction.

    摘要翻译： 本发明涉及用于扩增，检测和任选定量核酸的方法和装置。 在一个方面，该方法包括（a）提供反应体积，其包含（i）包含电化学活性导电聚合物的第一电极，能够与靶核酸杂交的第一单链核酸分子，其中所述第一核酸 分子共价连接到电化学活性导电聚合物上，和（ii）第二电极，（b）向反应体积提供反应混合物，反应混合物包含靶核酸，核酸聚合酶，氧化还原对， 和核酸扩增试剂，（c）扩增核酸，和（d）在核酸扩增反应期间至少测量一次第一电极的阻抗。

    Methods and Apparatus for Amplifying Nucleic Acids
    4.
    发明申请
    Methods and Apparatus for Amplifying Nucleic Acids 审中-公开

    公开(公告)号：US20190062808A1

    公开(公告)日：2019-02-28

    申请号：US16189731

    申请日：2018-11-13

    申请人： Nihan Aydemir ,  Jadranka Travas-Sejdic ,  Clive William Evans ,  David Edward Williams

    发明人： Nihan Aydemir ,  Jadranka Travas-Sejdic ,  Clive William Evans ,  David Edward Williams

    IPC分类号： C12Q1/686 ,  G01N27/02

    摘要： The present invention relates to methods and apparatuses for amplifying, detecting, and optionally quantifying, nucleic acids. In one aspect the method comprises (a) providing a reaction volume comprising (i) a first electrode comprising an electrochemically-active conducting polymer, a first single-stranded nucleic acid molecule capable of hybridizing to a target nucleic acid, wherein the first nucleic acid molecule is covalently attached to the electrochemically-active conducting polymer, and (ii) a second electrode, (b) providing a reaction mixture to the reaction volume, the reaction mixture comprising a target nucleic acid, a nucleic acid polymerase, a redox couple, and nucleic acid amplification reagents, (c) amplifying the nucleic acid, and (d) measuring the impedance of the first electrode at least once during the nucleic acid amplification reaction.

    MICROFABRICATED DEVICE
    6.
    发明申请
    MICROFABRICATED DEVICE 审中-公开
    微型设备

    公开(公告)号：US20100061870A1

    公开(公告)日：2010-03-11

    申请号：US11989984

    申请日：2006-08-01

    申请人： Mark B. Cannell ,  Ralph Paul Cooney ,  Paul Kilmartin ,  Christian Soeller ,  Jadranka Travas-Sejdic

    发明人： Mark B. Cannell ,  Ralph Paul Cooney ,  Paul Kilmartin ,  Christian Soeller ,  Jadranka Travas-Sejdic

    IPC分类号： F04B39/00

    CPC分类号： F04B43/043 ,  A61M5/14228 ,  B81B3/0021 ,  B81B2201/036 ,  F04B43/082 ,  F04B43/12

    摘要： A microfabricated device (10) includes a structure (12) defining a closed fluid delivery channel (14), the channel (14) having an inlet (16) and an opposed outlet (18). A conducting polymer actuator (20) is arranged within the fluid delivery channel (14). At least a part of the actuator (20) is configured to vary its cross sectional area in a direction transverse to a direction of fluid flow in the channel (14). An actuator control arrangement (22) is carried by the structure (12) for controlling the actuator (20) to cause the actuator (20) to expand and contract cyclically and sequentially along the length of the actuator (20) to vary the cross sectional area of the channel (14) cyclically and sequentially to effect a peristaltic pumping action to deliver fluid from the inlet (16) of the channel (14) to the outlet (18) of the channel (14).

    摘要翻译： 微加工装置（10）包括限定封闭的流体输送通道（14）的结构（12），所述通道（14）具有入口（16）和相对的出口（18）。 导电聚合物致动器（20）布置在流体输送通道（14）内。 致动器（20）的至少一部分被构造成在垂直于通道（14）中的流体流动方向的方向上改变其横截面积。 致动器控制装置（22）由结构（12）承载，用于控制致动器（20），以使致动器（20）沿致动器（20）的长度循环地和顺序地膨胀和收缩，以改变横截面 通道（14）的周边和顺序的区域以实现蠕动泵送动作，以将流体从通道（14）的入口（16）输送到通道（14）的出口（18）。