公开(公告)号：US06753142B2

公开(公告)日：2004-06-22

申请号：US09811842

申请日：2001-03-19

申请人： Charles J. Link ,  Tatiana Seregina ,  Nicholas N. Vahanian ,  James N. Higginbotham ,  W. Jay Ramsey ,  Bradley J. Powers ,  Sachet A. Shukla ,  Won Bin Young

发明人： Charles J. Link ,  Tatiana Seregina ,  Nicholas N. Vahanian ,  James N. Higginbotham ,  W. Jay Ramsey ,  Bradley J. Powers ,  Sachet A. Shukla ,  Won Bin Young

IPC分类号： C12Q168

CPC分类号： C12N15/10 ,  C12N15/1034

摘要： The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

摘要翻译： 本发明一般涉及用于鉴定差异蛋白表达模式的方法和组合物，并且伴随地直接或间接参与不同组织类型，疾病状态或诊断所需的其它细胞差异或药物治疗靶标的活性遗传区域 。

公开(公告)号：US06897020B2

公开(公告)日：2005-05-24

申请号：US09811842

申请日：2001-03-19

申请人： Charles J. Link ,  Tatiana Seregina ,  Nicholas N. Vahanian ,  James N. Higginbotham ,  W. Jay Ramsey ,  Bradley J. Powers ,  Sachet A. Shukla ,  Won Bin Young

发明人： Charles J. Link ,  Tatiana Seregina ,  Nicholas N. Vahanian ,  James N. Higginbotham ,  W. Jay Ramsey ,  Bradley J. Powers ,  Sachet A. Shukla ,  Won Bin Young

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/10 ,  C12N15/1034

摘要： The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

摘要翻译： 本发明一般涉及用于鉴定差异蛋白表达模式的方法和组合物，并且伴随地直接或间接参与不同组织类型，疾病状态或诊断所需的其它细胞差异或药物治疗靶标的活性遗传区域 。

公开(公告)号：US06875610B2

公开(公告)日：2005-04-05

申请号：US09871183

申请日：2001-05-31

申请人： James N. Higginbotham ,  Charles J. Link ,  William J. Ramsey

发明人： James N. Higginbotham ,  Charles J. Link ,  William J. Ramsey

IPC分类号： A61K48/00 ,  C12N15/86 ,  C12N15/861 ,  C07H21/04 ,  C12N5/10 ,  C12N15/63 ,  C12N15/64

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N7/00 ,  C12N2710/10343

摘要： The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

摘要翻译： 本发明包括病毒载体方法和组合物，其包含转运到受体细胞的反式互补复制无能病毒载体，优选腺病毒载体。 两个载体彼此互补，从而允许病毒复制，其协同组合增强载体中包含的遗传序列的基因递送和基因表达。

公开(公告)号：US07125549B2

公开(公告)日：2006-10-24

申请号：US10297099

申请日：2001-05-31

申请人： James N. Higginbotham ,  William J. Ramsey ,  Charles J. Link, Jr.

发明人： James N. Higginbotham ,  William J. Ramsey ,  Charles J. Link, Jr.

IPC分类号： C12N15/63 ,  C12N15/64 ,  C12N15/861 ,  C12N15/867 ,  C12N15/869

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N7/00 ,  C12N2710/10343

摘要： The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

公开(公告)号：US07078179B2

公开(公告)日：2006-07-18

申请号：US10838750

申请日：2004-05-04

申请人： Teresa Di Colandrea ,  Cherisa Meyer ,  Won-Bin Young ,  James N. Higginbotham ,  Mario Mautino ,  Charles J. Link, Jr.

发明人： Teresa Di Colandrea ,  Cherisa Meyer ,  Won-Bin Young ,  James N. Higginbotham ,  Mario Mautino ,  Charles J. Link, Jr.

IPC分类号： G01N33/567 ,  G01N33/53 ,  C12Q1/68 ,  C12N15/63 ,  C12N15/86

CPC分类号： G01N33/5023 ,  C07K16/40 ,  C07K2317/11 ,  C07K2317/23 ,  C12N9/1051 ,  C12N2799/027 ,  G01N2333/91102

摘要： A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-α Galactosyltransferase (αGT) enzyme for the production of αGalactosyl-(1,3)Galactosyl (αGal) epitopes on the surface of αGal-negative cells. These cells which are genetically modified to express the αGT enzyme and αGal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the αGal epitopes on the cell surface. Cells labeled with the anti-αGal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of αGal-positive cells from within a population of αGal-negative cells without the need to expose cells to antibiotics for any period of time. In addition, the specification provides a method for the production and purification of anti-αGal antibodies from chicken egg yolk.

摘要翻译： 描述了用于快速鉴定和分离细胞的方法，所述方法基于是否存在用于产生α-半乳糖基 - （1,3）半乳糖基（αGal）表位的异位表达的N-乙酰乳糖苷酶3-α半乳糖转移酶（αGT） 在αGal阴性细胞的表面。 然后通过识别和结合细胞表面上的αGal表位的抗体组合物来标记经遗传修饰以表达糖基化脂质和细胞表面蛋白质上的αGT表位的这些细胞。 用抗αGal抗体标记的细胞可以通过荧光激活细胞分选（FACS）进行分选，或通过磁性淘选技术分离。 该方法适用于从αGal阴性细胞群体内快速阳性或阴性选择αGal阳性细胞，无需在任何时间段内将细胞暴露于抗生素。 此外，本说明书提供了从鸡蛋黄制备和纯化抗αGal抗体的方法。