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27 条记录 (耗时 0.037 秒)

    Vehicles for stable transfer of green fluorescent protein gene and methods of use for same
    7.
    发明授权
    Vehicles for stable transfer of green fluorescent protein gene and methods of use for same 失效
    用于稳定转移绿色荧光蛋白基因的载体及其使用方法

    公开(公告)号：US06541197B2

    公开(公告)日：2003-04-01

    申请号：US08786531

    申请日：1997-01-21

    申请人： Charles J. Link, Jr. ,  John P. Levy ,  Suming Wang ,  Tatiana Seregina

    发明人： Charles J. Link, Jr. ,  John P. Levy ,  Suming Wang ,  Tatiana Seregina

    IPC分类号： C12Q170

    CPC分类号： C12N15/86 ,  C12N2740/13043 ,  C12Q1/6897

    摘要： The present invention describes an efficient retroviral or viral based method that allows easy and quick identification of gene transfer in living, transduced mammalian cells. Retroviral and viral vector producer cells were generated containing a gene for an improved humanized red-shifted, Green Fluorescent Protein (hRGFP) which increases the resulting fluorescence yield after excitation. This humanized, red-shifted GFP (hRGFP) gene was cloned into several vectors and transfected into various packaging cell lines to produce vibrant green fluorescence after excitation with blue light at 450-490 nm. These vectors represent a substantial advance over currently available gene transfer marking systems or wild-type GFP marker systems none of which have been stably transfected into cells.

    摘要翻译： 本发明描述了一种有效的基于逆转录病毒或病毒的方法，其允许容易且快速地鉴定生物，转导的哺乳动物细胞中的基因转移。 产生逆转录病毒和病毒载体生产细胞，其包含用于改善的人源化红移绿色荧光蛋白（hRGFP）的基因，其增加激发后产生的荧光产率。 将这种人源化的红移GFP（hRGFP）基因克隆到几个载体中并转染到各种包装细胞系中，以在450-490nm的蓝光激发后产生鲜艳的绿色荧光。 这些载体代表了目前可用的基因转移标记系统或野生型GFP标记系统的实质进展，其中没有一个已经稳定转染到细胞中。

    Herpes simplex virus amplicon mini-vector gene transfer system
    9.
    发明授权
    Herpes simplex virus amplicon mini-vector gene transfer system 失效
    单纯疱疹病毒扩增子小载体基因转移系统

    公开(公告)号：US5830727A

    公开(公告)日：1998-11-03

    申请号：US578500

    申请日：1995-12-26

    申请人： Suming Wang ,  Charles J. Link, Jr.

    发明人： Suming Wang ,  Charles J. Link, Jr.

    IPC分类号： C12N15/869 ,  C12N15/389 ,  C12N5/10 ,  C12N15/86

    CPC分类号： C12N15/86 ,  C12N2710/16643

    摘要： A novel HSV mini viral vector is disclosed. The vector comprises HSV and EBV genes which allow it to remain in episomal state, to have very high transfection and infection, and to tolerate up to 140 kb of foreign DNA. Techniques and genetic constructs for producing the vectors, for constructing the vectors and transfection and infection to recipient cells are disclosed.

    摘要翻译： 公开了一种新型HSV迷你病毒载体。 载体包含HSV和EBV基因，其允许其保持在附加状态，具有非常高的转染和感染，并且耐受高达140kb的外源DNA。 公开了用于产生载体的技术和遗传构建体，用于构建载体和转染和感染受体细胞。

    Antitumor vaccination using allogeneic tumor cells expressing alpha (1,3)-galactosyltransferase
    10.
    发明授权
    Antitumor vaccination using allogeneic tumor cells expressing alpha (1,3)-galactosyltransferase 有权
    使用表达α（1,3） - 半乳糖转移酶的同种异体肿瘤细胞的抗肿瘤疫苗接种

    公开(公告)号：US08535658B2

    公开(公告)日：2013-09-17

    申请号：US12878756

    申请日：2010-09-09

    申请人： Charles J. Link, Jr. ,  Tatiana Seregina ,  Gabriela Rossi

    发明人： Charles J. Link, Jr. ,  Tatiana Seregina ,  Gabriela Rossi

    IPC分类号： A61K48/00 ,  A61K39/39 ,  A61K31/70 ,  A61K39/395

    CPC分类号： A61K39/395 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2267/0331 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5156

    摘要： The invention relates to methods and compositions for causing the selective targeting and killing of tumor cells. Through ex vivo gene therapy protocols tumor cells are engineered to express an α (1,3) galactosyl epitope. The cells are then irradiated or otherwise killed and administered to a patient. The α galactosyl epitope causes opsonization of the tumor cell enhancing uptake of the opsonized tumor cell by antigen presenting cells which results in enhanced tumor specific antigen presentation. The animal's immune system thus is stimulated to produce tumor specific cytotoxic cells and antibodies which will attack and kill tumor cells present in the animal.

    摘要翻译： 本发明涉及用于引起肿瘤细胞选择性靶向和杀伤的方法和组合物。 通过离体基因治疗方案，肿瘤细胞被工程化以表达α（1,3）半乳糖基表位。 然后将细胞照射或以其它方式杀死并给予患者。 α半乳糖基表位导致肿瘤细胞的调理作用，通过抗原呈递细胞增强调理肿瘤细胞的摄取，导致增强的肿瘤特异性抗原呈递。 因此，动物的免疫系统被刺激以产生将攻击和杀死存在于动物中的肿瘤细胞的肿瘤特异性细胞毒性细胞和抗体。