摘要:
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
摘要翻译:编码修饰的萤光素酶多肽和底物的分离的多核苷酸。 OgLuc变体多肽与SEQ ID NO:1具有至少60%的氨基酸序列同一性,并且在对应于SEQ ID NO:1中的氨基酸的位置具有至少一个氨基酸取代.OgLuc变体多肽具有至少一个 相对于野生型Oplophorus萤光素酶的相应多肽,增强的发光,增强的信号稳定性和增强的蛋白质稳定性。
摘要:
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
摘要翻译:编码修饰的萤光素酶多肽和底物的分离的多核苷酸。 OgLuc变体多肽与SEQ ID NO:1具有至少60%的氨基酸序列同一性,并且在对应于SEQ ID NO:1中的氨基酸的位置具有至少一个氨基酸取代.OgLuc变体多肽具有至少一个 相对于野生型Oplophorus萤光素酶的相应多肽,增强的发光,增强的信号稳定性和增强的蛋白质稳定性。
摘要:
The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.