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公开(公告)号：US08343720B2
公开(公告)日：2013-01-01
申请号：US12657336
申请日：2010-01-19
申请人： Malcolm James Simons
发明人： Malcolm James Simons
CPC分类号： C12N15/1093 , C12Q1/6881 , C12Q2600/156 , G06F19/20 , G06F19/22 , Y10T436/143333
摘要： The present invention provides a method for identifying a set of target nucleotide sequences capable of identifying a member of a group of related nucleotide sequences, the method comprising the step of dividing the nucleotide sequence of each member of the group into a plurality of subsequences, wherein at least two of the subsequences overlap. The method is useful in generating probe sets capable of assigning alleles at HLA or KIR loci.
摘要翻译： 本发明提供了鉴定能够鉴定相关核苷酸序列组的成员的一组靶核苷酸序列的方法，所述方法包括将所述组的每个成员的核苷酸序列划分成多个子序列的步骤，其中 至少两个子序列重叠。 该方法可用于生成能够在HLA或KIR基因座上分配等位基因的探针组。
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公开(公告)号：US20110053789A1
公开(公告)日：2011-03-03
申请号：US12161166
申请日：2006-12-29
申请人： Malcolm James Simons
发明人： Malcolm James Simons
CPC分类号： C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a method for identifying a microarray probe set capable of identifying a member of a group of related nucleotide sequences, the method comprising the steps of providing a candidate probe set comprising at least one probe capable of differentially hybridizing to two or more members of the group of related nucleotide sequences, testing reactivity of the probe set against two or more members of the group of related nucleotide sequences, and observing the degree of difference in the patterns of reactivity of the probe set for the two or more members of the group of related nucleotide sequences.
摘要翻译： 本发明提供了一种用于鉴定能够鉴定一组相关核苷酸序列的成员的微阵列探针组的方法，所述方法包括以下步骤：提供候选探针组，所述候选探针组包括能够与两个或更多个成员差异杂交的至少一个探针 的相关核苷酸序列组，测试探针组对相关核苷酸序列组中的两个或多个成员的反应性，并观察探针组对于两个或更多个成员的反应性模式的差异程度 相关核苷酸序列组。
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公开(公告)号：US20060222166A1
公开(公告)日：2006-10-05
申请号：US11095775
申请日：2005-03-31
CPC分类号： H04M3/4938 , H04M7/006 , H04M2201/40
摘要： A telephony application host has a web service environment and a telephony application proxy. The web service environment is adapted to host one or more instances of a telephony application. The telephony application proxy is adapted to receive telephony messages associated with the one or more instances of the telephony application and to proxy each received telephony message into a web request. The telephony application is adapted to process the web request within the web service environment.
摘要翻译： 电话应用主机具有网络服务环境和电话应用代理。 网络服务环境适于承载电话应用的一个或多个实例。 电话应用代理适于接收与电话应用的一个或多个实例相关联的电话消息，并且将每个接收到的电话消息代理成web请求。 电话应用程序适于处理Web服务环境中的Web请求。
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4.发明申请Increment/decrement, chip select and selectable write to non-volatile memory using a two signal control protocol for an integrated circuit device 有权使用集成电路设备的两个信号控制协议，递增/递减，片选和可选写入非易失性存储器公开(公告)号：US20060023543A1
公开(公告)日：2006-02-02
申请号：US10946432
申请日：2004-09-21
申请人： James Simons
发明人： James Simons
IPC分类号： G11C7/00
CPC分类号： G06F13/4286
摘要： An integrated circuit having a device with an adjustable parameter utilizes a two signal control protocol to select the device, perform an up/down or increment/decrement of the parameter value with or without saving the parameter value in a non-volatile memory of the integrated circuit.
摘要翻译： 具有可调整参数的装置的集成电路利用两个信号控制协议来选择该装置，通过或不将该参数值保存在集成的非易失性存储器中来执行参数值的上/下或增量/减量 电路。
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公开(公告)号：US08673564B2
公开(公告)日：2014-03-18
申请号：US12663188
申请日：2008-06-06
申请人： Malcolm James Simons
发明人： Malcolm James Simons
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2600/156 , C12Q2535/131
摘要： The present invention is directed to in situ methods for providing a definitive haplotype of a subject. The haplotype information generated by the methods described herein is more accurate than that provided by prior art methods that only give an inferred haplotype. Accordingly, in one aspect the present invention provides an in situ method for obtaining genetic information for a polyploid subject, the method including the steps of obtaining a biological sample from the subject, the sample containing: (i) at least one paternally-derived DNA molecule, and/or (ii) at least one maternally-derived DNA molecule, analyzing any one or more of the paternally- or maternally-derived DNA molecules for nucleotide sequence information, wherein the step of analyzing determines whether any two DNA markers are present in cis on one chromosome, or in trans across two sister chromosomes. Use of in situ methods such as FISH allows for the provision of phase-specific information on DNA markers without recourse to methods for physically separating sister chromosomes. Applicants propose that method eliminates the problem of incorrect or misleading inferences concerning the phase of two or more loci within a haplotype, and allows for revelation of two or more participatory genes within a haplotype, uncomplicated by differences in modes of inheritance.
摘要翻译： 本发明涉及用于提供受试者的确定单体型的原位方法。 通过本文描述的方法产生的单倍型信息比仅提供推断的单倍型的现有技术方法提供的单体型信息更准确。 因此，一方面，本发明提供了一种用于获得多倍体受试者的遗传信息的原位方法，所述方法包括以下步骤：从受试者获得生物样品，所述样品包含：（i）至少一种亲本衍生的DNA 分子和/或（ii）至少一种母源衍生的DNA分子，分析任何一种或多种亲本或母源衍生的DNA分子中的核苷酸序列信息，其中分析步骤确定是否存在任何两个DNA标记物 在一条染色体上顺序，或穿过两条姊妹染色体。 使用诸如FISH之类的原位方法可以提供关于DNA标记的相位特异性信息，而无需采用物理分离姊妹染色体的方法。 申请人提出，该方法消除了关于单体型中两个或更多个位点的相位的错误或误导推论的问题，并且允许在单体型中揭示两个或更多个参与性基因，而不是通过继承模式的差异而复杂化。
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公开(公告)号：US20110033848A1
公开(公告)日：2011-02-10
申请号：US12663197
申请日：2008-06-06
申请人： Malcolm James Simons
发明人： Malcolm James Simons
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q1/6881 , C12Q2521/331 , C12Q2523/125
摘要： The present invention provides methods for obtaining epigenetic information for a polyploid subject, the method including the steps of obtaining a biological sample from the subject, the sample containing: (i) at least one paternally-derived DNA molecule and/or associated protein and/or, (ii) at least one maternally-derived DNA molecule and/or associated protein, analyzing any one or more of the paternally- or maternally-derived DNA molecules or associated proteins for the presence or absence of modifications, wherein the step of analyzing determines whether any two modifications are present in cis on one chromosome, or in trans across two sister chromosomes.
摘要翻译： 本发明提供了获得多倍体受试者的表观遗传信息的方法，所述方法包括从受试者获得生物样品的步骤，所述样品包含：（i）至少一种来源于亲本的DNA分子和/或相关蛋白质和/ 或者，（ii）至少一种母源衍生的DNA分子和/或相关蛋白，分析任何一种或多种所述亲本或母源衍生的DNA分子或相关蛋白，用于存在或不存在修饰，其中分析步骤 确定在一条染色体上或跨两条姐妹染色体中是否存在任何两种修饰。
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公开(公告)号：US20060105188A1
公开(公告)日：2006-05-18
申请号：US11065501
申请日：2005-02-24
申请人： James Simons
发明人： James Simons
IPC分类号： C08G18/00
CPC分类号： C09J175/06 , B32B7/12 , B32B27/32 , B32B27/36 , B32B37/12 , B32B2305/72 , C08G18/10 , C08G18/4202 , C08G18/4238 , C08G18/6644 , C08G2290/00 , Y10T428/31551 , Y10T428/31565 , Y10T428/31573 , Y10T428/3158 , Y10T428/31587 , Y10T428/31605 , Y10T428/31797
摘要： A curative containing two or more primary hydroxyl groups per molecule and one or more moieties derived from a secondary hydroxyl-containing polyol such as polypropylene glycol is prepared in a multistage process wherein the secondary hydroxyl-containing polyol is end-capped with an excess of a reactant such as a polybasic acid and the resulting functionalized intermediate is then reacted with a polyol such as glycerin or diethylene glycol containing primary hydroxyl groups. Such curatives may be used in combination with isocyanate-functionalized polyurethane prepolymers to provide two component adhesives useful for laminating thin films and/or foils.
摘要翻译： 在多级方法中制备每分子含有两个或更多个伯羟基和一个或多个衍生自含羟基的仲羟基的部分如聚丙二醇的固化剂，其中将含仲羟基的多元醇用过量的 然后将诸如多元酸和所得官能化中间体的反应物与多元醇如含有伯羟基的甘油或二甘醇反应。 这种固化剂可以与异氰酸酯官能化的聚氨酯预聚物组合使用，以提供用于层压薄膜和/或箔的双组分粘合剂。
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公开(公告)号：USD432384S
公开(公告)日：2000-10-24
申请号：US117394
申请日：2000-01-21
申请人： James Simons
设计人： James Simons
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公开(公告)号：US20100184057A1
公开(公告)日：2010-07-22
申请号：US12657336
申请日：2010-01-19
申请人： Malcolm James Simons
发明人： Malcolm James Simons
IPC分类号： C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6881 , C12Q2600/156 , G06F19/20 , G06F19/22 , Y10T436/143333
摘要： The present invention provides a method for identifying a set of target nucleotide sequences capable of identifying a member of a group of related nucleotide sequences, the method comprising the step of dividing the nucleotide sequence of each member of the group into a plurality of subsequences, wherein at least two of the subsequences overlap. The method is useful in generating probe sets capable of assigning alleles at HLA or KIR loci.
摘要翻译： 本发明提供了鉴定能够鉴定相关核苷酸序列组的成员的一组靶核苷酸序列的方法，所述方法包括将所述组的每个成员的核苷酸序列划分成多个子序列的步骤，其中 至少两个子序列重叠。 该方法可用于生成能够在HLA或KIR基因座上分配等位基因的探针组。
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公开(公告)号：US20090280484A1
公开(公告)日：2009-11-12
申请号：US12095907
申请日：2006-12-04
申请人： Malcolm James Simons
发明人： Malcolm James Simons
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683
摘要： The present invention is directed to methods for providing a definitive haplotype of a subject. The haplotype information generated by the methods described herein is more accurate than that provided by prior art methods that only give an inferred haplotype. Accordingly, in one aspect the present invention provides a method for determining a definitive haplotype of a subject the method including the steps of providing a substantially isolated haploid element from the subject, and obtaining nucleotide sequence information from the haploid element. Applicants propose that the use of a substantially isolated haploid element eliminates the problem of incorrect or misleading inferences concerning the phase of two or more loci within a haplotype, and allows for revelation of two or more participatory genes within a haplotype, uncomplicated by differences in modes of inheritance. The guarantee of strictly cis-phase associations is provided in the present methods by the use of a substantially isolated haploid element as starting material for sequence analysis.
摘要翻译： 本发明涉及提供受试者的确定单体型的方法。 通过本文描述的方法产生的单倍型信息比仅提供推断的单倍型的现有技术方法提供的单体型信息更准确。 因此，一方面，本发明提供了一种用于确定受试者的确定性单体型的方法，该方法包括以下步骤：从受试者提供基本上分离的单倍体元件，并从单倍体元件获得核苷酸序列信息。 申请人提出，使用基本上分离的单倍体元件消除了关于单体型中两个或更多个基因座的相位的不正确或误导性推断的问题，并且允许在单体型中揭示两种或更多种参与性基因，不同于模式差异 的继承。 在本方法中通过使用基本上分离的单倍体元件作为序列分析的起始材料，提供了严格顺式相关的保证。
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