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    Boresight verification device
    1.
    发明授权
    Boresight verification device 失效
    视觉验证设备

    公开(公告)号：US08739677B1

    公开(公告)日：2014-06-03

    申请号：US13374177

    申请日：2011-12-05

    申请人： James Travis Robbins ,  Luis J. Rodriguez ,  Danny L. Wilson

    发明人： James Travis Robbins ,  Luis J. Rodriguez ,  Danny L. Wilson

    IPC分类号： F41G1/00

    CPC分类号： F41A31/02 ,  F41G1/545

    摘要： A boresight verification device (BVD) comprised of a circular housing with a rear portion of smaller diameter and a front portion of larger diameter. The front portion securely holds a level. The circular housing also contains a plurality of spring plungers which grip the inside of a muzzle when BVD is inserted into a muzzle for use. A tooling ball provides a stable reference point.

    摘要翻译： 一种视轴验证装置（BVD），其包括具有较小直径后部的圆形壳体和较大直径的前部。 前部牢固地保持一个水平。 当BVD插入枪口用于使用时，圆形壳体还包含多个弹簧柱塞，其夹紧枪口内部。 工具球提供了稳定的参考点。

    THERMAL TREATMENT OF A CONTAMINATED VOLUME OF MATERIAL
    2.
    发明申请
    THERMAL TREATMENT OF A CONTAMINATED VOLUME OF MATERIAL 有权
    污染物质的热处理

    公开(公告)号：US20130310624A1

    公开(公告)日：2013-11-21

    申请号：US13673793

    申请日：2012-11-09

    申请人： David Glynn Thomas ,  Thomas Reece Peargin ,  Roderick James Travis

    发明人： David Glynn Thomas ,  Thomas Reece Peargin ,  Roderick James Travis

    IPC分类号： A62D3/40

    CPC分类号： A62D3/40 ,  B09B3/0066 ,  B09B3/0083 ,  B09C1/06 ,  B09C1/08 ,  E21B21/066 ,  F23C99/006 ,  F23G7/14 ,  F23G2209/24

    摘要： A method for treating a volume of porous contaminated material is described. A combustible mixture (24) comprising a porous matrix and a distributed combustible contaminant is conveyed to a reaction vessel (12) having an ignition system (16). The ignition system is activated to initiate a smoldering combustion process in the reaction vessel and an oxidizer is distributed into the reaction vessel to sustain the smoldering combustion process. The porous matrix may comprise drill cuttings (324) with residual drilling fluid.

    摘要翻译： 描述了一种处理多孔污染物质的方法。 包含多孔基质和分布的可燃性污染物的可燃混合物（24）被输送到具有点火系统（16）的反应容器（12）。 点火系统被激活以启动反应容器中的阴燃燃烧过程，并且将氧化剂分配到反应容器中以维持阴燃燃烧过程。 多孔基质可以包括具有残余钻井液的钻屑（324）。

    Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis
    3.
    发明授权
    Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis 失效
    牙龈卟啉单胞菌脯氨酰三肽基肽酶核酸

    公开(公告)号：US06875851B1

    公开(公告)日：2005-04-05

    申请号：US09518550

    申请日：2000-03-03

    申请人： James Travis ,  Jan Potempa ,  Agnieszka Banbula

    发明人： James Travis ,  Jan Potempa ,  Agnieszka Banbula

    IPC分类号： A61K39/00 ,  C12N9/48 ,  C01N21/02

    CPC分类号： C12N9/48 ,  A61K39/00

    摘要： The present invention provides isolated polypeptides, prolyl tripeptidyl-peptidases, and active analogs, active fragments or active modifications thereof, having amidolytic activity for cleavage of a peptide bond present in a target peptide having at least 30 amino acids. Isolated nucleic acid fragments encoding isolated prolyl tripeptidyl-peptidases are also provided, as are methods of reducing growth of a bacterium by inhibiting a prolyl tripeptidyl-peptidase.

    摘要翻译： 本发明提供分离的多肽，脯氨酰三肽基肽酶和活性类似物，其活性片段或其活性修饰物，其具有用于切割存在于具有至少30个氨基酸的靶肽中的肽键的酰胺分解活性。 还提供了编码分离的脯氨酰三肽基肽酶的分离的核酸片段，以及通过抑制脯氨酰三肽基肽酶来减少细菌生长的方法。

    Polypeptide having amidolytic activity for a serpin
    4.
    发明授权
    Polypeptide having amidolytic activity for a serpin 失效
    针对丝氨酸蛋白酶抑制剂具有酰胺分解活性的多肽

    公开(公告)号：US06833262B1

    公开(公告)日：2004-12-21

    申请号：US10030330

    申请日：2001-10-19

    申请人： James Travis ,  Jan S. Potempa ,  Daniel C. Nelson

    发明人： James Travis ,  Jan S. Potempa ,  Daniel C. Nelson

    IPC分类号： C12N952

    CPC分类号： C12N9/6472 ,  A61K39/00

    摘要： An isolated oral bacterial polypeptide having amidolytic activity for cleavage of denatured polypeptides and nondenatured serpin polypeptides and particularly a human &agr;1-proteinase inhibitor polypeptide is provided. The mature polypeptide of the invention has a molecular weight of about 70 kD to about 80 kD. Also provided is an isolated nucleic acid sequence encoding the oral bacterial polypeptide of the invention, methods for identifying inhibitors of the polypeptide and compositions such as immunogenic compositions and inhibitor compositions.

    摘要翻译： 提供了具有用于切割变性多肽和非变性丝氨酸蛋白酶多肽，特别是人α1-蛋白酶抑制剂多肽的酰胺分解活性的分离的口腔细菌多肽。 本发明的成熟多肽具有约70kD至约80kD的分子量。 还提供了编码本发明的口腔细菌多肽的分离的核酸序列，用于鉴定多肽的抑制剂的方法和组合物，例如免疫原性组合物和抑制剂组合物。

    Porphyromonas gingivalis arginine-specific proteinase coding sequences
    5.
    发明授权
    Porphyromonas gingivalis arginine-specific proteinase coding sequences 有权
    牙龈卟啉单胞菌精氨酸特异性蛋白酶编码序列

    公开(公告)号：US06274718B1

    公开(公告)日：2001-08-14

    申请号：US09490931

    申请日：2000-01-25

    申请人： James Travis ,  Jan Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff

    发明人： James Travis ,  Jan Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff

    IPC分类号： C07H2104

    CPC分类号： C12N9/52

    摘要： Provided herein is a nucleotide sequence encoding an Arg-specific gingipain, said Arg-gingipain characterized as having an apparent molecular mass of 50 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and an apparent molecular mass of 44 kDa as estimated by gel filtration chromatography, said gingipain-1 having amidolytic and proteolytic activity for cleavage after arginine residues and having no amidolytic and/or proteolytic activity for cleavage after lysine residues, wherein the amidolytic and/or proteolytic activity is inhibited by cysteine protease group-specific inhibitors including iodoacetamide, iodoacetic acid, N-ethylmaleimide, leupeptin, antipain, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, TLCK, TPCK, p-aminobenzamidine, N-chlorosuccinamide, and chelating agents including EDTA and EGTA, wherein the amidolytic and/or proteolytic activity of said gingipain-1 is not sensitive to inhibition by human cystatin C, &agr;2-macroglobulin, &agr;1-proteinase inhibitor, antithrombin III, &agr;2-antiplasmin, serine protease group-specific inhibitors including diisopropylfluorophosphate, phenylmethyl sulfonylfluoride and proteolytic activities of ginigipan-1 are stabilized by Ca2+ and 3,4-diisochlorocoumarin, and wherein the amidolytic and/or wherein the amidolytic and/or proteolytic activities of said gingipain-1 are stimulated by glycine-containing peptides and glycine analogues, and methods for preparation. As specifically exemplified, high and low molecular weight forms of Arg-gingipains and sequences encoding them are prepared from Porphyromonas gingivalis. Said high molecular weight Arg-gingipain comprises a proteolytic component as described hereinabove and at least one hemagglutinin component.

    摘要翻译： 本文提供了编码Arg-特异性姜黄素的核苷酸序列，所述Arg-营养蛋白的特征在于通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳估计的表观分子量为50kDa，通过凝胶过滤色谱法估测的表观分子量为44kDa 所述鹅膏苷-1具有用于在精氨酸残基之后切割的酰胺分解和蛋白水解活性，并且在赖氨酸残基之后不具有用于切割的酰胺解和/或蛋白水解活性，其中所述酰胺分解和/或蛋白水解活性被半胱氨酸蛋白酶组特异性抑制剂包括碘乙酰胺， 碘乙酸，N-乙基马来酰亚胺，亮抑酶肽，抗胰蛋白酶，反式环氧琥珀酰基-L-亮氨酰氨基 - （4-胍基）丁烷，TLCK，TPCK，对氨基脒，N-氯代琥珀酰胺和螯合剂，包括EDTA和EGTA，其中酰胺分解和 /或所述鹅蛋白-1的蛋白水解活性对人半胱氨酸蛋白酶抑制剂C，α2-巨球蛋白，alph α-淀粉样蛋白酶抑制剂，抗凝血酶III，α2-抗纤溶酶原，丝氨酸蛋白酶组特异性抑制剂，包括二异丙基氟磷酸酯，苯基甲基磺酰氟和金丝雀蛋白-1的蛋白水解活性由Ca2 +和3,4-二氯氯香豆素稳定，其中酰胺分解和/或其中 甘氨酸-1的酰胺分解和/或蛋白水解活性由含甘氨酸的肽和甘氨酸类似物及其制备方法刺激。 如具体示例的，由牙龈卟啉单胞菌制备高分子量和低分子量形式的Arg- ing ip ains和编码它们的序列。 所述高分子量的精胍碱包含如上所述的蛋白水解组分和至少一种血凝素组分。

    Porphyromonas gingivalis arginine-specific proteinase
    6.
    发明授权
    Porphyromonas gingivalis arginine-specific proteinase 失效
    牙龈卟啉单胞菌精氨酸特异性蛋白酶

    公开(公告)号：US5523390A

    公开(公告)日：1996-06-04

    申请号：US119361

    申请日：1993-09-10

    申请人： James Travis ,  Jan S. Potempa ,  Philip J. Barr ,  Nadine Pavloff

    发明人： James Travis ,  Jan S. Potempa ,  Philip J. Barr ,  Nadine Pavloff

    IPC分类号： C12N9/52 ,  C12N9/14 ,  C07H19/00 ,  C12N9/48

    CPC分类号： C12N9/52

    摘要： Provide herein is a substantially pure gingipain-1 preparation, gingipain-1 being characterized as having an apparent molecular mass of 50 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and an apparent molecular mass of 44 kDa as estimated by gel filtration chromatography, said gingipain-1 having amidolytic and proteolytic activity for cleavage after arginine residues and having no amidolytic and/or proteolytic activity for cleavage after lysine residues, wherein the amidolytic and/or proteolytic activity is inhibited by cysteine protease group-specific inhibitors including iodoacetamide, iodoacetic acid, N-ethylmaleimide, leupeptin, antipain, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, TLCK, TPCK, p-aminobenzamidine, N-chlorosuccinamide, and chelating agents including EDTA and EGTA, wherein the amidolytic and/or proteolytic activity of said gingipain-1 is not sensitive to inhibition by human cystatin C, .alpha.2-macroglobulin, .alpha.1-proteinase inhibitor, antithrombin III, .alpha.2-antiplasmin, serine protease group-specific inhibitors including diisopropylfluorophosphate, phenylmethyl sulfonylfluoride and 3,4-diisochlorocoumarin, and wherein the amidolytic and/or proteolytic activities of gingipain-1 are stabilized by Ca.sup.2+ and wherein the amidolytic and/or proteolytic activities of said gingipain-1 are stimulated by glycine-containing peptides and glycine analogues, and methods for preparation. As specifically exemplified, Arg-gingipain-1 and Arg-gingipain-2 are purified from Porphyromonas gingivalis, and the mature Arg-gingipain-2 has an amino acid sequence as given in SEQ ID NO:5 from amino acid 1 through amino acid 510. Also provided are nucleic acid sequences encoding Arg-gingipain-2. The nucleotide coding sequence of the mature Arg-gingipain-2 is given in SEQ ID NO:4, from nucleotide 1630 through nucleotide 3105.

    摘要翻译： 本文提供的是基本上纯的姜黄素-1制剂，姜黄素-1的特征在于通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳估计的表观分子量为50kDa，通过凝胶过滤色谱法估计的表观分子量为44kDa，所述 姜黄素-1具有酰胺分解和蛋白水解活性，用于精氨酸残基之后的切割，并且在赖氨酸残基之后不具有用于切割的酰氨基裂解和/或蛋白水解活性，其中酰胺分解和/或蛋白水解活性被半胱氨酸蛋白酶组特异性抑制剂抑制，包括碘乙酰胺，碘乙酸 ，N-乙基马来酰亚胺，亮抑酶肽，抗胰蛋白酶，反式 - 环氧琥珀酰基-L-亮氨酰氨基 - （4-胍基）丁烷，TLCK，TPCK，对氨基苯甲脒，N-氯代琥珀酰胺和包括EDTA和EGTA的螯合剂，其中酰胺分解和/ 所述姜黄素-1的蛋白水解活性对人半胱氨酸蛋白酶抑制剂C，α2-巨球蛋白，α1-蛋白酶 e抑制剂，抗凝血酶III，α2-抗血纤维蛋白酶，丝氨酸蛋白酶组特异性抑制剂，包括二异丙基氟磷酸酯，苯基甲基磺酰氟和3,4-二氯代香豆素，其中盖蛋白-1的酰胺分解和/或蛋白水解活性由Ca 2+稳定，其中酰胺分解 和/或所述鹅蛋白-1的蛋白水解活性由含甘氨酸的肽和甘氨酸类似物及其制备方法刺激。 如具体示例，从牙龈卟啉单胞菌中纯化Arg-gingipain-1和Arg-gingipain-2，并且成熟的Arg-gingipain-2具有如SEQ ID NO：5中从氨基酸1至氨基酸510所给出的氨基酸序列 还提供了编码Arg-gingipain-2的核酸序列。 成熟Arg-gingipain-2的核苷酸编码序列在SEQ ID NO：4中由核苷酸1630至核苷酸3105给出。

    Determination of oxidized .alpha.-1-proteinase inhibitor in serum or plasma
    10.
    发明授权
    Determination of oxidized .alpha.-1-proteinase inhibitor in serum or plasma 失效
    血清或血浆中氧化的α-1蛋白酶抑制剂的测定

    公开(公告)号：US4605616A

    公开(公告)日：1986-08-12

    申请号：US654966

    申请日：1984-09-27

    申请人： James Travis

    发明人： James Travis

    IPC分类号： C12Q1/37 ,  C12Q1/38 ,  C12N9/99

    CPC分类号： C12Q1/37

    摘要： A new method of determining oxidized .alpha.-1-proteinase inhibitor in serum or plasma for use in studying the development of chronic obstructive lung disease is disclosed. Levels of oxidized .alpha.-1-proteinase inhibitor indicate the potential for emphysema development with higher levels showing a decrease in lung protection against elastolytic enzymes such as elastase. This method can be used for patients with a potential for chronic obstructive lung disease rather than having to use bronchial lavage methods for such patients. No other method is known to exist for determining oxidized .alpha.-1-proteinase inhibitor in serum or plasma.

    摘要翻译： 公开了一种在血清或血浆中测定氧化型α-1蛋白酶抑制剂用于研究慢性阻塞性肺疾病发展的新方法。 氧化的α-1蛋白酶抑制剂的水平表明肺气肿发展的潜力较高，显示出对弹性蛋白酶等弹性蛋白酶的肺保护作用下降。 这种方法可用于患有慢性阻塞性肺疾病的患者，而不是为此类患者使用支气管灌洗方法。 没有其他方法可用于测定血清或血浆中的氧化型α-1蛋白酶抑制剂。