公开(公告)号：US20120196285A1

公开(公告)日：2012-08-02

申请号：US13362299

申请日：2012-01-31

申请人： Patricia Okamoto ,  Jan Godoski ,  Thomas Scholl

发明人： Patricia Okamoto ,  Jan Godoski ,  Thomas Scholl

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/1003

摘要： Methods for enriching specific microparticles, such as fetal microparticles or disease specific microparticles, in a biological sample are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds specific microparticles, and separating fractions of the biological sample, wherein the fraction that contains the binding molecule is enriched for the specific microparticles. Also disclosed are methods for enriching fetal nucleic acids by enriching fetal microparticles in a fraction of the biological sample and isolating nucleic acids from the enriched fraction. Methods for facilitating prenatal diagnosis of fetal chromosomal abnormalities are disclosed. In certain embodiments, the methods include combining a biological sample with a molecule that binds fetal microparticles, separating fractions of the biological sample, isolating nucleic acids from the fraction enriched for fetal microparticles, and analyzing the nucleic acids for the presence of a mutation.

摘要翻译： 公开了在生物样品中富集特定微粒例如胎儿微粒或疾病特异性微粒的方法。 在某些实施方案中，所述方法包括将生物样品与结合特定微粒的分子结合，并分离生物样品的级分，其中含有结合分子的级分富集特定的微粒。 还公开了通过富集生物样品的一部分中的胎儿微粒并从富集部分分离核酸来富集胎儿核酸的方法。 公开了促进胎儿染色体异常产前诊断的方法。 在某些实施方案中，所述方法包括将生物样品与结合胎儿微粒的分子组合，分离生物样品的级分，从富集胎儿微粒的级分中分离核酸，以及分析核酸以存在突变。

公开(公告)号：US20120264628A1

公开(公告)日：2012-10-18

申请号：US13362655

申请日：2012-01-31

申请人： Patricia Okamoto ,  Jan Godoski ,  Thomas Scholl

发明人： Patricia Okamoto ,  Jan Godoski ,  Thomas Scholl

IPC分类号： C07H1/08 ,  G01N21/64 ,  C07H21/04 ,  C40B30/04 ,  G01N27/62 ,  G01N27/26 ,  C12Q1/68 ,  G01N21/76

CPC分类号： C12M33/14 ,  C12M47/02 ,  C12N15/1017 ,  C12Q1/6806 ,  C12Q2531/113

摘要： Embodiments of the present invention provide methods for the enrichment of rare microparticles, cells, or nucleic acids from a complex mixture using serial size exclusion filtration. Also provided are less invasive methods for detecting chromosomal or genetic abnormalities in a fetus, by enriching fetal microparticles in maternal plasma using serial size exclusion filtration, and isolating and analyzing the fetal nucleic acids from the fetal microparticles. Methods for diagnosis of diseases such as cancer are also provided, including enriching disease specific microparticles in the patient's plasma using serial size exclusion filtration, and isolating and analyzing the nucleic acids from the disease specific microparticles.

摘要翻译： 本发明的实施方案提供了使用连续大小排阻过滤从复杂混合物中富集稀有微粒，细胞或核酸的方法。 还提供了用于检测胎儿染色体或遗传异常的侵入性较低的方法，通过使用连续大小排阻过滤富集母体血浆中的胎儿微粒，并从胎儿微粒分离和分析胎儿核酸。 还提供了用于诊断疾病如癌症的方法，包括使用连续大小排阻过滤来富集患者血浆中的疾病特异性微粒，以及分离和分析来自疾病特异性微粒的核酸。