公开(公告)号：US08680252B2

公开(公告)日：2014-03-25

申请号：US12518595

申请日：2007-12-10

申请人： Mark A. Emalfarb ,  Peter J. Punt ,  Cornelia Van Zeijl ,  Cornelius Van Den Hondel ,  Jan Verdoes ,  Richard P. Burlingame

发明人： Mark A. Emalfarb ,  Peter J. Punt ,  Cornelia Van Zeijl ,  Cornelius Van Den Hondel ,  Jan Verdoes ,  Richard P. Burlingame

IPC分类号： C12P1/00 ,  C12P21/04 ,  C07H21/02

CPC分类号： C12N15/80 ,  C07K16/00 ,  C07K2317/14 ,  C07K2319/30 ,  C12N9/58 ,  C12N15/1082 ,  C12P21/02 ,  C12R1/645 ,  C12Y204/0201 ,  C12Y401/01023

摘要： The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.

摘要翻译： 本发明提供了在丝状真菌宿主中表达和随后筛选DNA文库，特别是合成的，基因组和cDNA文库的方法。 特别地，本发明提供了载体，宿主菌株和用于表达和筛选复合DNA文库的方法，包括但不限于表达一种，两种或更多种可变成分和/或由两种或两种以上制备的组合（组合）文库 或更多的亚图书馆（例如，用于表达和筛选免疫球蛋白（包括整个免疫球蛋白蛋白的片段和衍生物）和其他受体或复合DNA文库或文库的文库）。 本发明可用于表达和筛选大量蛋白质和蛋白质复合物，包括人类蛋白质。 本发明还涉及新的真菌蛋白酶序列。

公开(公告)号：US20120030839A1

公开(公告)日：2012-02-02

申请号：US12518595

申请日：2007-12-10

申请人： Mark A. Emalfarb ,  Peter J. Punt ,  Cornelia Van Zeijl ,  Cornelius Van Den Hondel ,  Jan Verdoes ,  Richard P. Burlingame

发明人： Mark A. Emalfarb ,  Peter J. Punt ,  Cornelia Van Zeijl ,  Cornelius Van Den Hondel ,  Jan Verdoes ,  Richard P. Burlingame

IPC分类号： A01H5/00 ,  C12N1/15 ,  C12N1/14 ,  C40B50/06 ,  C40B20/00 ,  C40B30/00 ,  C40B30/04 ,  C12N1/21 ,  C12N9/58 ,  C12N15/80 ,  C12N15/85 ,  C12N15/81 ,  C40B10/00 ,  C40B40/02 ,  A61K39/00 ,  C12P21/00 ,  C07H21/04 ,  C07K16/40 ,  C12P21/06 ,  C12N15/00 ,  A61P37/04 ,  C12N15/63

CPC分类号： C12N15/80 ,  C07K16/00 ,  C07K2317/14 ,  C07K2319/30 ,  C12N9/58 ,  C12N15/1082 ,  C12P21/02 ,  C12R1/645 ,  C12Y204/0201 ,  C12Y401/01023

摘要： The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.

摘要翻译： 本发明提供了在丝状真菌宿主中表达和随后筛选DNA文库，特别是合成的，基因组和cDNA文库的方法。 特别地，本发明提供了载体，宿主菌株和用于表达和筛选复合DNA文库的方法，包括但不限于表达一种，两种或更多种可变成分和/或由两种或两种以上制备的组合（组合）文库 或更多的亚图书馆（例如，用于表达和筛选免疫球蛋白（包括整个免疫球蛋白蛋白的片段和衍生物）和其他受体或复合DNA文库或文库的文库）。 本发明可用于表达和筛选大量蛋白质和蛋白质复合物，包括人类蛋白质。 本发明还涉及新的真菌蛋白酶序列。