公开(公告)号：US20060281155A1

公开(公告)日：2006-12-14

申请号：US11509662

申请日：2006-08-25

申请人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Suto ,  Janos Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Nee Igloy

发明人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Suto ,  Janos Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Nee Igloy

IPC分类号： C12P7/62

CPC分类号： C12R1/645 ,  C12P7/62

摘要： The present invention relates to a new microbial process for the preparation of the compound formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6β-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6β-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

公开(公告)号：US20050124051A1

公开(公告)日：2005-06-09

申请号：US11014658

申请日：2004-12-16

申请人： Antonia Jekkel ,  Gabor Ambrus ,  Eva Ilkoy ,  Ildiko Horvath ,  Attila Konya ,  Istvan Szabo ,  Zsuzsanna Nagy ,  Gyula Horvath ,  Julia Mozes ,  Istvan Barta ,  Gyorgy Somogyi ,  Janos Salat ,  Sandor Boros

发明人： Antonia Jekkel ,  Gabor Ambrus ,  Eva Ilkoy ,  Ildiko Horvath ,  Attila Konya ,  Istvan Szabo ,  Zsuzsanna Nagy ,  Gyula Horvath ,  Julia Mozes ,  Istvan Barta ,  Gyorgy Somogyi ,  Janos Salat ,  Sandor Boros

IPC分类号： C12P7/62 ,  C07C69/30 ,  C12P1/04 ,  C12P7/42 ,  C12P17/06 ,  C12R1/29 ,  C12R1/31 ,  C12N1/21 ,  C12N9/18

CPC分类号： C12R1/29 ,  C12P7/42 ,  C12P17/06

摘要： The present invention relates to a new microbial process for the preparation of compound of formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged culture of a strain which is able to 6β-hydroxylate the compound of formula (II) in aerobic fermentation and by the separation and purification of the product of formula (I) formed in the course of the biocoversion. The latter comprises the cultivation of a Micromonospora strain which is able to 6β-hydroxylate a compound of general formula (II)—wherein R is as defined above—at 25-32° C. on a nutrient medium containing available carbon—and nitrogen sources and mineral salts, thereafter feeding the substrate to be transformed into the developing culture, then hydroxilating the substrate until finishing of the bioconversion, then separating the compound of formula (I) from the culture broth and, if desired, purifying the same.

摘要翻译： 本发明涉及一种由通式（II）化合物制备式（I）化合物的新型微生物方法，其中R代表碱金属或铵离子，通过浸没培养的能够 在好氧发酵中通过分解和纯化在生物转化过程中形成的式（I）的产物，使式（II）化合物6β-羟化。 后者包括培养微孢子虫菌株，其能够在含有可用碳源和氮源的营养培养基上在25-32℃下将通式（II）化合物（其中R如上定义）6β-羟基化 然后将待转化的底物喂入显影培养物，然后将底物氢化，直到生物转化完成，然后从培养液中分离式（I）化合物，如果需要，将其纯化。

公开(公告)号：US06245921B1

公开(公告)日：2001-06-12

申请号：US09497806

申请日：2000-02-03

申请人： Istvan Barta ,  Aniko Tegdes ,  Valeria Szell ,  Csaba Szabo ,  Edit Nagy Nee Arvai ,  Vilmos Keri ,  David Leonov ,  IIdiko Lang ,  Margit Bidlo Nee Igloy ,  Gyula Jerkovich ,  Janos Salat

发明人： Istvan Barta ,  Aniko Tegdes ,  Valeria Szell ,  Csaba Szabo ,  Edit Nagy Nee Arvai ,  Vilmos Keri ,  David Leonov ,  IIdiko Lang ,  Margit Bidlo Nee Igloy ,  Gyula Jerkovich ,  Janos Salat

IPC分类号： C07D31500

CPC分类号： C12P17/162

摘要： A process for the isolation of the pseudomonic acid A antibiotic of pharmaceutical quality from the culture broth of one of the pseudomonic acid A-producing species of the Pseudomonas bacterium genus comprising the extraction of the biosynthesized pseudomonic acid A from the culture broth at acidic pH with a chlorinated aliphatic hydrocarbon or isobutyl acetate, followed by purification, is disclosed. The invention includes processes for purification of the isolated pseudomonic acid A, including (a) by the distribution of the evaporated extract residue between the aqueous-alcohol and some aliphatic or aromatic hydrocarbon, and then the extraction of the increased water-containing aqueous-alcoholic phase with methylene chloride, ethyl acetate, or isobutyl acetate; (b) by the extraction of the extract with aqueous ammonium hydrogen carbonate, alkali metal hydroxide or ammonium hydroxide solution and the acidification of the resulting alkaline aqueous extract, then reextraction again with a chlorinated aliphatic hydrocarbon or isobutyl acetate; and (c) by the concentration of the extract and the recrystallization of the crystalline pseudomonic acid A in a mixture of isobutyl acetate and petroleum ether, or acetonitrile, or aqueous acetonitrile.

摘要翻译： 从假单胞菌属的假单胞菌素A产生物种之一的培养液中分离出假单胞菌A抗生素药物质量的方法，包括在酸性pH下从培养液中提取生物合成的假单胞菌酸A， 公开了氯化脂族烃或乙酸异丁酯，然后纯化。 本发明包括纯化分离的假单胞菌酸A的方法，包括（a）通过在含水醇和某些脂族或芳族烃之间分配蒸发的提取物残渣，然后提取含水的含水醇 与二氯甲烷，乙酸乙酯或乙酸异丁酯反应; （b）通过用碳酸氢铵水溶液，碱金属氢氧化物或氢氧化铵溶液萃取提取物，并将得到的碱性水提取物酸化，然后用氯化脂族烃或乙酸异丁酯再次萃取; 和（c）通过提取物的浓度和结晶假单胞菌酸A在乙酸异丁酯和石油醚或乙腈或乙腈水溶液的混合物中的重结晶。