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公开(公告)号：US20060099614A1
公开(公告)日：2006-05-11
申请号：US11223848
申请日：2005-09-08
申请人： Peter Gill , Javaid Hussain , Adam Long
发明人： Peter Gill , Javaid Hussain , Adam Long
CPC分类号： C12Q1/6881 , C12Q1/6858 , C12Q2600/156 , C12Q2537/149 , C12Q2531/113
摘要： The invention provides improved techniques for investigating DNA samples, which offers improved sensitivity and specifity. The invention provides a method of investigating single nucleotide polymorphisms in a sample of DNA, the method comprising contacting the DNA containing sample with at least one first set of primers, amplifying the DNA using those primers to give an amplified product, contacting at least a portion of the amplified product with at least one second set of primers, amplifying the DNA using those second set of primers to give a further amplified product and examining one or more characteristics of the further amplified product, one or more of the primers of the first set of primers including a locus specific portion and a further portion, the locus specific portion of one of those one or more of the primers annealing to one side of the SNP under investigation.
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公开(公告)号：US20090170106A1
公开(公告)日：2009-07-02
申请号：US12272434
申请日：2008-11-17
申请人： Peter GILL , Javaid Hussain , Adam Long
发明人： Peter GILL , Javaid Hussain , Adam Long
CPC分类号： C12Q1/6881 , C12Q1/6858 , C12Q2600/156 , C12Q2537/149 , C12Q2531/113
摘要： The invention provides improved techniques for investigating DNA samples, which offers improved sensitivity and specifity.The invention provides a method of investigating single nucleotide polymorphisms in a sample of DNA, the method comprising contacting the DNA containing sample with at least one first set of primers, amplifying the DNA using those primers to give an amplified product, contacting at least a portion of the amplified product with at least one second set of primers, amplifying the DNA using those second set of primers to give a further amplified product and examining one or more characteristics of the further amplified product, one or more of the primers of the first set of primers including a locus specific portion and a further portion, the locus specific portion of one of those one or more of the primers annealing to one side of the SNP under investigation.
摘要翻译： 本发明提供了用于研究DNA样品的改进技术，其提供了改进的灵敏度和特异性。 本发明提供了一种研究DNA样品中单核苷酸多态性的方法，该方法包括使含有DNA的样品与至少一组第一组引物接触，使用这些引物扩增DNA以产生扩增产物，使至少部分 的扩增产物与至少一个第二组引物扩增DNA，使用第二组引物扩增DNA，得到进一步扩增产物并检查进一步扩增产物的一个或多个特征，第一组的一个或多个引物 包括基因座特异部分和另外部分的引物的一个或多个引物中的一个的基因座特异性部分退火到正在研究的SNP的一侧。
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公开(公告)号：US20070178497A1
公开(公告)日：2007-08-02
申请号：US11615046
申请日：2006-12-22
申请人： Peter Gill , Javaid Hussain , Adam Long , Gillian Tully
发明人： Peter Gill , Javaid Hussain , Adam Long , Gillian Tully
CPC分类号： C12Q1/6881 , C12Q1/6858 , C12Q2600/156 , G16B30/00 , G16B40/00 , C12Q2537/143 , C12Q2535/125
摘要： The invention provides a method for obtaining additional information about DNA mixtures arising from a variety of sources and/or a variety of concentrations. In particular, the invention provides a method for indicating the likelihood that a DNA mixture arose from sources of a defined type where: the DNA mixture is formed by DNA samples from more than one source, the method involving the determination of the identity of the alleles present at a locus for the DNA in the mixture; determining a first probability function for the situation where the DNA mixture is formed from samples arising from the given person and from a first other person; determining a second probability function for the situation where the DNA mixture is formed from samples arising from a second other person and a first other person; using the first probability function as numerator and the second probability function as denominator in determining a likelihood ratio for the mixture having arisen from the defined type of sources considered in the first probability function; determining such likelihood ratios for a plurality of loci; and combining the likelihood ratios to give a combined likelihood ratio for the mixture having arisen from the defined type of sources considered in the first probability function.
摘要翻译： 本发明提供了一种获得关于由各种来源和/或多种浓度产生的DNA混合物的附加信息的方法。 特别地，本发明提供了一种用于指示DNA混合物从定义类型的来源产生的可能性的方法，其中：DNA混合物由来自多于一个来源的DNA样品形成，所述方法涉及确定等位基因的身份 存在于混合物中DNA的位点; 确定由给定人和第一另一个人产生的样品形成DNA混合物的情况的第一概率函数; 确定由第二另一个人和第一另一个人产生的样品形成DNA混合物的情况的第二概率函数; 使用第一概率函数作为分子，并且将第二概率函数作为分母来确定从在第一概率函数中考虑的限定类型的源产生的混合物的似然比; 确定多个基因座的似然比; 并且将似然比组合以给出从在第一概率函数中考虑的所定义类型的源产生的混合物的组合似然比。
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公开(公告)号：US20050014183A1
公开(公告)日：2005-01-20
申请号：US10892854
申请日：2004-07-15
申请人： Peter Gill , Javaid Hussain , Adam Long
发明人： Peter Gill , Javaid Hussain , Adam Long
CPC分类号： C12Q1/6876 , C12Q2600/156 , C12Q2600/16
摘要： Primer mixtures and methods of investigating single nucleotide polymorphisms in samples of DNA are provided having particular primer sequences. The primer mixtures and methods are aimed at achieving optimised performance in multiplexes.
摘要翻译： 提供引物混合物和研究DNA样品中单核苷酸多态性的方法，其具有特定的引物序列。 引物混合物和方法旨在实现多路复用中的优化性能。
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公开(公告)号：US20060003369A1
公开(公告)日：2006-01-05
申请号：US11153837
申请日：2005-06-15
申请人： Peter Gill , Javaid Hussain , Adam Long , Gillian Tully
发明人： Peter Gill , Javaid Hussain , Adam Long , Gillian Tully
IPC分类号： C12Q1/68
CPC分类号： G16B30/00
摘要： A method of evaluating primers for use in the amplification of DNA sequences, a method of producing a mixture of primers for such a purpose and a method of amplifying a plurality of DNA sequences using a mixture of primers is provided in which the evaluation involves performing one or more initial evaluations, obtaining a plurality of SNP sites and related potential primer identities which are passes, generating primers corresponding to those primer identities and conducting a test amplification process using those primers in conjunction with a further evaluation of the results, the pass primers therefrom forming a pool of primer candidates from which one or more primers for use in the amplification of DNA sequences are selected. The invention provides a faster, cheaper and more versatile technique for developing multiplexes, particularly for forensic science type investigations.
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