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18 条记录 (耗时 0.016 秒)

    Plant tubby-like proteins
    1.
    发明授权
    Plant tubby-like proteins 有权
    植物卵巢样蛋白

    公开(公告)号：US08410337B2

    公开(公告)日：2013-04-02

    申请号：US12372231

    申请日：2009-02-17

    申请人： Jei-Fu Shaw ,  Chia-ping Lai ,  Po-Hong Chen

    发明人： Jei-Fu Shaw ,  Chia-ping Lai ,  Po-Hong Chen

    IPC分类号： A01H5/00 ,  C12N5/14 ,  C12N15/82

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N15/8271 ,  Y02A40/146

    摘要： An isolated polypeptide containing an amino acid sequence at least 70% identical to a Tubby-like protein (SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11), and an isolated nucleic acid encoding the polypeptide. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing one of SEQ ID NOs:1-11; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant having a reduced level(s) of one or more of the Tubby-like proteins. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.

    摘要翻译： 一种分离的多肽，其含有与Tubby样蛋白质（SEQ ID NO：1,2,3,4,5,6,7,8,9,10或11）至少70％相同的氨基酸序列，以及 分离的编码多肽的核酸。 公开了一种分离的核酸，其在严格条件下与含有SEQ ID NO：1-11之一的探针杂交; 或其互补序列。 还公开了（1）含有这种核酸的转化细胞或转基因植物和（2）具有降低的一种或多种类似蛋白质的水平的转化细胞或转基因植物。 在本发明的范围内也是用于制备转化细胞或转基因植物的方法。

    Mutant-type lipases and applications thereof
    3.
    发明授权
    Mutant-type lipases and applications thereof 失效

    公开(公告)号：US07049122B2

    公开(公告)日：2006-05-23

    申请号：US10233443

    申请日：2002-09-04

    申请人： Rey-Chang Chang ,  Shu-Jen Chou ,  Jei-Fu Shaw

    发明人： Rey-Chang Chang ,  Shu-Jen Chou ,  Jei-Fu Shaw

    IPC分类号： C12N9/20

    CPC分类号： C12N9/18 ,  C12N9/20 ,  C12Y301/01003

    摘要： The present invention provides mutant-type lipases which demonstrate superior lipolytic and esterific activities. The mutant-type lipases are characterized by an amino acid alteration at the residue immediately followed either the serine residue or the histidine residue or both residues of the Ser-His-Asp catalytic triad. The Ser-His-Asp catalytic triad is known to be the three residues, although occur far apart in the amino acid sequence of a lipase, that contribute to the hydrolytic activity in the active site of the lipase. The amino acid residue that follows the serine residue of the Ser-His-Asp catalytic triad is alanine. The amino acid residue that follows the histidine residue of the Ser-His-Asp catalytic triad is isoleucine. The wild-type lipase is preferably originated from Staphylococcus, particularly Staphylococcus epidermindis. The present invention also relates to a method for preparing the mutant-type lipases by site-directed mutagenesis using PCR and a method for utilizing the mutant-type lipase to catalyze synthesis of flavor esters to be used in food industry.

    Recombinant Candida rugosa lipases
    4.
    发明授权
    Recombinant Candida rugosa lipases 有权

    公开(公告)号：US07052879B2

    公开(公告)日：2006-05-30

    申请号：US09943857

    申请日：2001-08-31

    申请人： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Shye-Jye Tang

    发明人： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Shye-Jye Tang

    IPC分类号： C12P19/34 ,  C07H21/04

    CPC分类号： C12N9/20 ,  C07K2319/00

    摘要： The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.

    Plant tubby-like proteins
    5.
    发明申请
    Plant tubby-like proteins 失效
    植物卵巢样蛋白

    公开(公告)号：US20050014266A1

    公开(公告)日：2005-01-20

    申请号：US10763042

    申请日：2004-01-21

    申请人： Jei-Fu Shaw ,  Chia-Ping Lai

    发明人： Jei-Fu Shaw ,  Chia-Ping Lai

    IPC分类号： C07H21/04 ,  C07K14/415 ,  C12N15/82 ,  C07K14/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63 ,  C12N15/87

    CPC分类号： C07K14/415 ,  C07H21/04 ,  C12N15/8271

    摘要： An isolated polypeptide containing an amino acid sequence at least 70% identical to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, and an isolated nucleic acid encoding the polypeptide. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing one of SEQ ID NOs:1-11; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant lacking one or more of SEQ ID NOs:1-11. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.

    摘要翻译： 含有与SEQ ID NO：1,2,3,4,5,6,7,8,9,10或11至少70％相同的氨基酸序列的分离多肽和编码该多肽的分离的核酸。 公开了一种分离的核酸，其在严格条件下与含有SEQ ID NO：1-11之一的探针杂交; 或其互补序列。 还公开了（1）含有这种核酸的转化细胞或转基因植物和（2）缺失SEQ ID NO：1-11中的一个或多个的转化细胞或转基因植物。 在本发明的范围内也是用于制备转化细胞或转基因植物的方法。

    RECOMBINANT CANDIDA RUGOSA LIPASES
    7.
    发明申请
    RECOMBINANT CANDIDA RUGOSA LIPASES 失效
    重组甘地达葛根药

    公开(公告)号：US20090053795A1

    公开(公告)日：2009-02-26

    申请号：US11841492

    申请日：2007-08-20

    申请人： Jei-Fu Shaw ,  Guan-chiun Lee ,  Shye-Jye Tang

    发明人： Jei-Fu Shaw ,  Guan-chiun Lee ,  Shye-Jye Tang

    IPC分类号： C12N15/55 ,  C12N1/21 ,  C12N1/19

    CPC分类号： C12N9/20 ,  C07K2319/00

    摘要： The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.

    摘要翻译： 本发明的特征在于分离的核酸，其包含编码假丝酵母假单胞菌脂肪酶的突变DNA，其中突变DNA与编码假丝酵母脂肪酶的野生型DNA具有80％同一性，并且包括至少12个（例如13,15 ，17或全部）对应于野生型DNA中的CTG密码子的通用丝氨酸密码子。 每个通用丝氨酸密码子独立地是TCT，TCC，TCA，TCG，AGT或AGC。 假丝酵母粗脂肪酶可以是1号，2号，3号，4号，5号或8号念珠菌。

    Novel trehalose synthase from Picrophilus torridus and methods of use thereof
    8.
    发明申请
    Novel trehalose synthase from Picrophilus torridus and methods of use thereof 有权
    来自Picrophilus torridus的新型海藻糖合成酶及其使用方法

    公开(公告)号：US20080182311A1

    公开(公告)日：2008-07-31

    申请号：US11490702

    申请日：2006-07-21

    申请人： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Yi-Shan Chen

    发明人： Jei-Fu Shaw ,  Guan-Chiun Lee ,  Yi-Shan Chen

    IPC分类号： C12N9/00 ,  C07H21/04 ,  C12N15/00 ,  C12N1/00

    CPC分类号： C12N9/90 ,  C12Y504/99016

    摘要： The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned from Picrophilus torridus. More particularly, the present invention discloses the DNA sequence for the Picrophilus torridus trehalose synthase gene, PTTS, which when expressed in a heterologous host such as Escherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.

    摘要翻译： 本发明涉及从天竺葵（Trrophicus torridus）克隆的新型耐酸耐热海藻糖合成酶的分离和功能鉴定。 更具体地，本发明公开了用于在异源宿主如大肠杆菌中表达的天竺葵torridus海藻糖合酶基因PTTS的DNA序列，其提供催化通过分子内转糖基化直接相互转化麦芽糖和海藻糖的酶活性。 此外，本发明教导了使用PTTS生产海藻糖以及生产包含海藻糖的各种有用化合物的方法。

    Plant tubby-like proteins
    9.
    发明授权
    Plant tubby-like proteins 失效
    植物卵巢样蛋白

    公开(公告)号：US07314756B2

    公开(公告)日：2008-01-01

    申请号：US10763042

    申请日：2004-01-21

    申请人： Jei-Fu Shaw ,  Chia-Ping Lai

    发明人： Jei-Fu Shaw ,  Chia-Ping Lai

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87 ,  C12N5/14

    CPC分类号： C07K14/415 ,  C07H21/04 ,  C12N15/8271

    摘要： An isolated polypeptide containing an amino acid sequence at least 70% identical to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, and an isolated nucleic acid encoding the polypeptide are disclosed. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing SEQ ID NO:20; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant lacking the polypeptide encoded by the nucleic acid. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.

    摘要翻译： 包含与SEQ ID NO：1,2,3,4,5,6,7,8,9,10或11至少70％相同的氨基酸序列的分离多肽和编码该多肽的分离的核酸是 披露 公开了一种分离的核酸，其在严格条件下与含有SEQ ID NO：20的探针杂交; 或其互补序列。 还公开了（1）含有这种核酸的转化细胞或转基因植物和（2）缺乏由核酸编码的多肽的转化细胞或转基因植物。 在本发明的范围内也是用于制备转化细胞或转基因植物的方法。