公开(公告)号：US07396677B2

公开(公告)日：2008-07-08

申请号：US10703368

申请日：2003-11-07

申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/22 ,  B01L3/00 ,  B01L3/02

CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

摘要： A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法和装置。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。

公开(公告)号：US20050112583A1

公开(公告)日：2005-05-26

申请号：US10703368

申请日：2003-11-07

申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

IPC分类号： B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N20060101 ,  G01N35/10

CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

摘要： A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法和装置。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。

公开(公告)号：US20080268458A1

公开(公告)日：2008-10-30

申请号：US12169618

申请日：2008-07-08

申请人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

发明人： Tim Patno ,  Jennifer Hollenstein ,  Christian Kronshage ,  Christopher Khoury ,  Mark Weber ,  Tom Westberg ,  William Cork

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  B01L3/5025 ,  B01L3/5027 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1097 ,  C12Q2563/155 ,  C12Q2565/519

摘要： A method is provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

摘要翻译： 提供了一种制备用于检测预定靶核酸的测试样品的方法。 该方法包括以下步骤：提供包含与纳米颗粒连接的寡核苷酸并提供含有测试样品和测试探针的杂交单元的测试探针，其中所述杂交单元还包括目标样品基底和连接到第一侧的分布歧管 的基底。 该方法还包括以下步骤：将加工流体歧管夹紧到杂交单元的分配歧管上，使测试样品变性并制备用于通过在处理流体源歧管之间泵送多个处理流体来检测预定靶核酸的测试样品 以及分配多肽以将测试探针和预定靶核酸与目标样品底物杂交，以洗涤杂交样品并扩增杂交样品的可检测参数。