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27 条记录 (耗时 0.033 秒)

    Fluorescence dyes and their applications for whole-cell fluorescence screening assays for caspases, peptidases, proteases and other enzymes and the use thereof
    2.
    发明授权
    Fluorescence dyes and their applications for whole-cell fluorescence screening assays for caspases, peptidases, proteases and other enzymes and the use thereof 失效
    荧光染料及其在半胱氨酸蛋白酶，肽酶，蛋白酶和其他酶的全细胞荧光筛选测定中的应用及其用途

    公开(公告)号：US06984718B2

    公开(公告)日：2006-01-10

    申请号：US10138375

    申请日：2002-05-06

    申请人： Han-Zhong Zhang ,  Sui Xiong Cai ,  John A. Drewe ,  Wu Yang

    发明人： Han-Zhong Zhang ,  Sui Xiong Cai ,  John A. Drewe ,  Wu Yang

    IPC分类号： C07K5/00 ,  G01N33/573

    CPC分类号： C07D493/10 ,  C07D311/82 ,  C07K5/06191 ,  C07K5/0827 ,  C07K5/1027 ,  C07K7/06 ,  Y10T436/142222

    摘要： The present invention relates to novel fluorescent dyes, novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of caspases and other enzymes involved in apoptosis in whole cells, cell lines and tissue samples derived from any living organism or organ. The reporter molecules and assay processes can be used in drug screening procedures to identify compounds which act as inhibitors or inducers of the caspase cascade in whole cells or tissues. The reagents and assays described herein are also useful for determining the chemosensitivity of human cancer cells to treatment with chemotherapeutic drugs. The present invention also relates to novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of type 2 methionine aminopeptidase, HIV protease, adenovirus protease, HSV-1 protease, HCMV protease and HCV protease.

    摘要翻译： 本发明涉及新的荧光染料，新的荧光和荧光报道分子和新的酶测定方法，其可用于检测来自任何活生物体的全细胞，细胞系和组织样品中参与细胞凋亡的半胱天冬酶和其它酶的活性 或器官。 报告分子和测定方法可以用于药物筛选程序中，以鉴定在整个细胞或组织中作为半胱天冬酶级联的抑制剂或诱导剂的化合物。 本文所述的试剂和测定法也可用于确定人类癌细胞对用化学治疗药物治疗的化学敏感性。 本发明还涉及可用于检测2型甲硫氨酸氨基肽酶，HIV蛋白酶，腺病毒蛋白酶，HSV-1蛋白酶，HCMV蛋白酶和HCV蛋白酶的活性的新的荧光和荧光报道分子和新的酶测定方法。

    Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for caspases and other enzymes and the use thereof
    5.
    发明授权
    Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for caspases and other enzymes and the use thereof 有权
    荧光或荧光报告分子及其在半胱氨酸蛋白酶和其他酶的全细胞荧光筛选测定中的应用及其用途

    公开(公告)号：US06335429B1

    公开(公告)日：2002-01-01

    申请号：US09521650

    申请日：2000-03-08

    申请人： Sui Xiong Cai ,  John F. W. Keana ,  John A. Drewe ,  Han-Zhong Zhang

    发明人： Sui Xiong Cai ,  John F. W. Keana ,  John A. Drewe ,  Han-Zhong Zhang

    IPC分类号： C07K1702

    CPC分类号： G01N15/1456 ,  C12Q1/68 ,  G01N33/533 ,  G01N2015/1006 ,  G01N2015/1488 ,  Y10S436/80

    摘要： The present invention relates to novel fluorescent dyes, novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of caspases and other enzymes involved in apoptosis in whole cells, cell lines and tissue samples derived from any living organism or organ. The reporter molecules and assay processes can be used in drug screening procedures to identify compounds which act as inhibitors or inducers of the caspase cascade in whole cells or tissues. The reagents and assays described herein are also useful for determining the chemosensitivity of human cancer cells to treatment with chemotherapeutic drugs. The present invention also relates to novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of type 2 methionine aminopeptidase, dipeptidyl peptidase IV, calpain, aminopeptidase, HIV protease, adenovirus protease, HSV-1 protease, HCMV protease and HCV protease.

    摘要翻译： 本发明涉及新的荧光染料，新的荧光和荧光报道分子和新的酶测定方法，其可用于检测来自任何活生物体的全细胞，细胞系和组织样品中参与细胞凋亡的半胱天冬酶和其它酶的活性 或器官。 报告分子和测定方法可以用于药物筛选程序中，以鉴定在整个细胞或组织中作为半胱天冬酶级联的抑制剂或诱导剂的化合物。 本文所述的试剂和测定法也可用于确定人类癌细胞对用化学治疗药物治疗的化学敏感性。 本发明还涉及可用于检测2型甲硫氨酸氨基肽酶，二肽基肽酶IV，钙蛋白酶，氨基肽酶，HIV蛋白酶，腺病毒蛋白酶，HSV-1蛋白酶的活性的新型荧光和荧光报告分子和新的酶测定方法， HCMV蛋白酶和HCV蛋白酶。

    Microwave apparatus for heating contained liquid
    6.
    发明授权
    Microwave apparatus for heating contained liquid 失效
    用于加热的微波炉包含液体

    公开(公告)号：US4742202A

    公开(公告)日：1988-05-03

    申请号：US506169

    申请日：1983-06-20

    申请人： Nancy L. Campbell ,  John G. Pinto ,  John A. Drewe ,  Gerald W. Ghing ,  Franklin R. Borkat

    发明人： Nancy L. Campbell ,  John G. Pinto ,  John A. Drewe ,  Gerald W. Ghing ,  Franklin R. Borkat

    IPC分类号： H05B6/64 ,  H05B6/80 ,  H05B6/78

    CPC分类号： H05B6/64

    摘要： An apparatus is provided for heating contained liquid, and more particula for thawing frozen blood components which are contained in a bag. The heating apparatus includes a microwave oven which has a cavity for receiving objects to be heated. A track is mounted in the oven cavity and has a peak and a valley with respect to the cavity floor. A tray is provided for supporting the liquid container. A device is provided for pivotally supporting the tray within the cavity with the tray in engagement with the track means, and a device is located within the oven for imparting relative motion between the tray and the track. With this arrangement the liquid container can be moved in a rocking motion to cause a mixing between the warm and cold portions of the liquid, and in particular with regard to frozen blood will cause the thawed liquid portions of the blood to flow over the still frozen portions so as to dissipate heat therebetween.

    摘要翻译： 提供一种用于加热所含液体的装置，更具体地说，用于解冻包含在袋中的冷冻血液成分。 加热装置包括微波炉，其具有用于接收被加热物体的空腔。 轨道安装在烤箱腔中，并且具有相对于空腔底板的峰谷。 提供用于支撑液体容器的托盘。 提供一种装置，用于将托盘枢转地支撑在空腔内，托盘与轨道装置接合，并且装置位于炉内以便在托盘和轨道之间施加相对运动。 通过这种布置，液体容器可以以摇摆的方式运动，从而在液体的温暖部分之间进行混合，特别是关于冻结的血液将导致解冻的液体部分流过静止的 以便在它们之间散热。