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公开(公告)号：US5804433A
公开(公告)日：1998-09-08
申请号：US735963
申请日：1996-10-23
CPC分类号： C12N9/0036 , C10G32/00
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供了一种新的黄素还原酶，其已从红球菌属菌株sp。 IGTS8并纯化至均匀。 黄素还原酶在含有机硫化合物的化石燃料的生物催化脱硫中有用。 该方法包括以下步骤：（1）使化石燃料与含有能够切割碳 - 硫键的生物催化剂和速率增加量的本发明的黄素还原酶的水相接触，从而形成化石燃料和水相 混合物; （2）将混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
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公开(公告)号：US5733773A
公开(公告)日：1998-03-31
申请号：US662810
申请日：1996-06-12
申请人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
发明人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02 , C12N1/20
CPC分类号： C12N9/0036 , C10G32/00 , C12P1/00
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.
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公开(公告)号：US5919683A
公开(公告)日：1999-07-06
申请号：US105057
申请日：1998-06-26
CPC分类号： C12N9/0036 , C10G32/00
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供了一种新的黄素还原酶，其已从红球菌属菌株sp。 IGTS8并纯化至均匀。 黄素还原酶在含有机硫化合物的化石燃料的生物催化脱硫中有用。 该方法包括以下步骤：（1）使化石燃料与含有能够切割碳 - 硫键的生物催化剂和速率增加量的本发明的黄素还原酶的水相接触，从而形成化石燃料和水相 混合物; （2）将混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
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公开(公告)号：US06274372B1
公开(公告)日：2001-08-14
申请号：US09304214
申请日：1999-05-03
IPC分类号： C10G3200
CPC分类号： C12N9/0036 , C10G32/00
摘要： The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon—sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon—sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供了一种新的黄素还原酶，其已从红球菌属菌株sp。 IGTS8并纯化至均匀。 黄素还原酶在含有机硫化合物的化石燃料的生物催化脱硫中有用。 该方法包括以下步骤：（1）使化石燃料与含有能够切割碳 - 硫键的生物催化剂和速率增加量的本发明的黄素还原酶的水相接触，从而形成化石燃料和水相 混合物; （2）将混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
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5.发明授权公开(公告)号：US06235519B1
公开(公告)日：2001-05-22
申请号：US09030995
申请日：1998-02-26
IPC分类号： C12N114
CPC分类号： C12N9/0004 , C10G32/00 , C12P1/06 , C12P3/00 , C12R1/365
摘要： The present invention relates to an isolated nucleic acid molecule which encodes one or more of the enzymes which catalyze one or more steps in the desulfurization of thiophene, or a homologue or active fragment thereof. The invention also includes a recombinant microorganism containing one or more such heterologous nucleic acid molecules. The invention also provides a method for desulfurizing a fossil fuel containing thiophene and/or one or more substituted thiophenes. The method comprises contacting the fossil fuel with an organism containing a recombinant nucleic acid molecule which encodes an enzyme which catalyzes the desulfurization of thiophene.
摘要翻译： 本发明涉及编码一种或多种催化噻吩脱硫中的一个或多个步骤的酶或其同系物或活性片段的分离的核酸分子。 本发明还包括含有一种或多种这样的异源核酸分子的重组微生物。 本发明还提供了一种使含有噻吩和/或一种或多种取代的噻吩的化石燃料脱硫的方法。 该方法包括使化石燃料与含有重组核酸分子的生物接触，所述重组核酸分子编码催化噻吩脱硫的酶。
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公开(公告)号：US5985650A
公开(公告)日：1999-11-16
申请号：US25527
申请日：1998-02-18
申请人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
发明人： Charles H. Squires , Wan Ji , Lei Xi , Beatrice C. Ortego , Olga S. Pogrebinsky , Kevin A. Gray , John D. Childs
IPC分类号： C12N15/09 , C10G32/00 , C10L1/04 , C12N1/21 , C12N9/02 , C12N15/00 , C12N15/53 , C12P1/00 , C12R1/01 , C12R1/19 , C12S1/02
CPC分类号： C12N9/0036 , C10G32/00 , C12P1/00
摘要： The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of:a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture;b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; andc) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.
摘要翻译： 本发明涉及通过向生物催化剂中加入黄素蛋白来提高化石燃料脱硫反应速率的发现。 本发明涉及提高含有机硫化合物的化石燃料脱硫速率的方法，包括以下步骤：a）使化石燃料与含有能够裂解碳 - 硫键的生物催化剂的水相接触， 增加量的黄素蛋白，从而形成化石燃料和水相混合物; b）将步骤（a）的混合物保持在足以通过生物催化剂裂解有机硫分子的碳 - 硫键的条件下，从而导致具有降低的有机硫含量的化石燃料; 和c）从所得水相中分离具有降低的有机硫含量的化石燃料。 本发明还涉及含有一种或多种重组DNA分子的重组微生物，所述重组DNA分子编码能够脱硫含有有机硫分子的化石燃料并且编码黄素蛋白的生物催化剂。 本发明还涉及包含（a）能够使含有有机硫分子的化石燃料脱硫的生物催化剂和（b）黄素蛋白的组合物。
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公开(公告)号：US5952208A
公开(公告)日：1999-09-14
申请号：US851088
申请日：1997-05-05
CPC分类号： C12N9/0051 , C10G32/00 , C12N15/52
摘要： The present invention provides novel recombinant pseudomonads which contain a heterologous nucleic acid molecule comprising a nucleotide sequence encoding one or more desulfurization enzymes which are components of a biodesulfurization catalyst. The invention also provides a method of desulfurizing a carbonaceous material, such as a fossil fuel, which comprises organosulfur compounds. The method includes the steps of The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a recombinant biocatalyst which is capable of cleaving carbon-sulfur bonds and, optionally, a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organosulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
摘要翻译： 本发明提供新的重组假单胞菌，其含有包含作为生物脱硫催化剂组分的一种或多种脱硫酶的核苷酸序列的异源核酸分子。 本发明还提供一种包含有机硫化合物的含碳材料如化石燃料的脱硫方法。 该方法包括以下步骤：该方法包括以下步骤：（1）使化石燃料与含有能够裂解碳 - 硫键的重组生物催化剂的水相和任选的黄素蛋白接触，从而形成化石燃料和水性 相混合物 （2）在足以使生物催化剂裂解有机硫分子的碳 - 硫键的条件下保持混合物，从而导致具有降低的有机硫含量的化石燃料; 和（3）从所得水相中分离具有降低的有机硫含量的化石燃料。
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