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    T7 phage peptide display system and uses thereof
    4.
    发明授权
    T7 phage peptide display system and uses thereof 有权
    T7噬菌体肽显示系统及其用途

    公开(公告)号：US09217145B2

    公开(公告)日：2015-12-22

    申请号：US12121176

    申请日：2008-05-15

    申请人： Alexander V. Sokoloff ,  Jon Asher Wolff ,  James John Ludtke

    发明人： Alexander V. Sokoloff ,  Jon Asher Wolff ,  James John Ludtke

    IPC分类号： A01K67/027 ,  C12N15/00 ,  C12Q1/70 ,  C12N15/10 ,  C07K14/005

    CPC分类号： C12N15/1037 ,  C07K14/005 ,  C12N2795/10222

    摘要： A bacteriophage T7 display vector for expressing and displaying an exogenous peptide, wherein the display vector comprises a polynucleotide encoding a bacteriophage T7 tail fiber protein p17 in which a hepatocyte-targeting determinant sequence is inactivated, and wherein a cloning site is contained in a coding sequence for the tail fiber protein p17 or in a coding sequence for a capsid protein p10B. Also provided are a host cell containing the display vector as described above, a bacteriophage T7 particle comprising at least one copy of a p17 protein which comprises an exogenous peptide displayed thereon, or at least one copy of a p10 protein with an exogenous peptide displayed thereon, wherein a hepatocyte-targeting determinant sequence on the bacteriophage T7 tail fiber protein p17 is inactivated. The present invention further provides a viral lysate containing assembled bacteriophage T7 particle described above, and a method for determining if a candidate peptide is a liver-targeting peptide, or for screening for liver targeting peptides.

    摘要翻译： 用于表达和显示外源肽的噬菌体T7展示载体，其中所述显示载体包含编码噬菌体T7尾纤维蛋白质p17的多核苷酸，其中肝细胞靶向决定簇序列失活，并且其中克隆位点包含在编码序列 对于尾纤维蛋白p17或在衣壳蛋白p10B的编码序列中。 还提供了含有如上所述的显示载体的宿主细胞，包含至少一个拷贝的p17蛋白的噬菌体T7颗粒，其包含其上显示的外源肽，或至少一个拷贝的p10蛋白与其上显示的外源肽 其中噬菌体T7尾纤维蛋白p17上的肝细胞靶向决定簇序列失活。 本发明还提供了含有上述组装的噬菌体T7颗粒的病毒裂解液，以及用于确定候选肽是否为肝靶向肽或用于筛选肝脏靶向肽的方法。

    Novel T7 Phage Peptide Display System and Uses Thereof
    5.
    发明申请
    Novel T7 Phage Peptide Display System and Uses Thereof 有权
    新型T7噬菌体肽显示系统及其用途

    公开(公告)号：US20090113562A1

    公开(公告)日：2009-04-30

    申请号：US12121176

    申请日：2008-05-15

    申请人： Alexander V. Sokoloff ,  Jon Asher Wolff ,  James John Ludtke

    发明人： Alexander V. Sokoloff ,  Jon Asher Wolff ,  James John Ludtke

    IPC分类号： A01K67/027 ,  C12N15/00 ,  C12N5/06 ,  C12Q1/70

    CPC分类号： C12N15/1037 ,  C07K14/005 ,  C12N2795/10222

    摘要： A bacteriophage T7 display vector for expressing and displaying an exogenous peptide, wherein the display vector comprises a polynucleotide encoding a bacteriophage T7 tail fiber protein p17 in which a hepatocyte-targeting determinant sequence is inactivated, and wherein a cloning site is contained in a coding sequence for the tail fiber protein p17 or in a coding sequence for a capsid protein p10B. Also provided are a host cell containing the display vector as described above, a bacteriophage T7 particle comprising at least one copy of a p17 protein which comprises an exogenous peptide displayed thereon, or at least one copy of a p10 protein with an exogenous peptide displayed thereon, wherein a hepatocyte-targeting determinant sequence on the bacteriophage T7 tail fiber protein p17 is inactivated. The present invention further provides a viral lysate containing assembled bacteriophage T7 particle described above, and a method for determining if a candidate peptide is a liver-targeting peptide, or for screening for liver targeting peptides.

    摘要翻译： 用于表达和显示外源肽的噬菌体T7展示载体，其中所述显示载体包含编码噬菌体T7尾纤维蛋白质p17的多核苷酸，其中肝细胞靶向决定簇序列失活，并且其中克隆位点包含在编码序列 对于尾纤维蛋白p17或在衣壳蛋白p10B的编码序列中。 还提供了含有如上所述的显示载体的宿主细胞，包含至少一个拷贝的p17蛋白的噬菌体T7颗粒，其包含其上显示的外源肽，或至少一个拷贝的p10蛋白与其上显示的外源肽 其中噬菌体T7尾纤维蛋白p17上的肝细胞靶向决定簇序列失活。 本发明还提供了含有上述组装的噬菌体T7颗粒的病毒裂解液，以及用于确定候选肽是否为肝靶向肽或用于筛选肝脏靶向肽的方法。