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1.发明申请公开(公告)号：US20140234851A1
公开(公告)日：2014-08-21
申请号：US14236974
申请日：2012-08-03
CPC分类号： C12Q1/6897 , G01N33/566 , G01N33/582 , G01N33/6872
摘要： The present invention provides modular extracellular sensors, nucleic acids encoding such sensors, and cells expressing such sensors, and methods of employing such sensors and cells for detecting extracellular ligands. In certain embodiments, the extracellular sensors comprise a ligand binding domain, a transmembrane domain, a protease domain, a protease cleavage site, and a transcription factor. In other embodiments, a pair of extracellular receptors is provided where both receptors contain a ligand binding domain and transmembrane domain, and one receptor contains a protease cleavage site and a transcription factor and the other receptor contains a protease domain.
摘要翻译： 本发明提供了模块化细胞外传感器，编码这种传感器的核酸和表达这种传感器的细胞，以及使用这种传感器和细胞来检测细胞外配体的方法。 在某些实施方案中，细胞外传感器包括配体结合结构域，跨膜结构域，蛋白酶结构域，蛋白酶切割位点和转录因子。 在其它实施方案中，提供了一对细胞外受体，其中两个受体含有配体结合结构域和跨膜结构域，一个受体含有蛋白酶切割位点和转录因子，另一个受体含有蛋白酶结构域。
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公开(公告)号：US09732392B2
公开(公告)日：2017-08-15
申请号：US14236974
申请日：2012-08-03
IPC分类号： C12P21/02 , C12Q1/68 , G01N33/566 , G01N33/58 , G01N33/68 , C12N9/48 , C12N9/50 , C12N5/10 , C12N15/79
CPC分类号： C12Q1/6897 , G01N33/566 , G01N33/582 , G01N33/6872
摘要： The present invention provides modular extracellular sensors, nucleic acids encoding such sensors, and cells expressing such sensors, and methods of employing such sensors and cells for detecting extracellular ligands. In certain embodiments, the extracellular sensors comprise a ligand binding domain, a transmembrane domain, a protease domain, a protease cleavage site, and a transcription factor. In other embodiments, a pair of extracellular receptors is provided where both receptors contain a ligand binding domain and transmembrane domain, and one receptor contains a protease cleavage site and a transcription factor and the other receptor contains a protease domain.
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公开(公告)号：US07851196B2
公开(公告)日：2010-12-14
申请号：US11739441
申请日：2007-04-24
IPC分类号： C12N7/02 , C12N7/00 , C12N7/04 , C12N15/00 , C12N1/02 , C12N13/00 , C12Q1/70 , A01N63/00 , A61K39/00 , A61K39/12 , A61K39/235 , C07H21/02
CPC分类号： C12N7/00 , C12N15/86 , C12N2750/14143 , C12N2750/14151
摘要： The present invention provides methods of purifying encapsidated virus, e.g., viral particles comprising viral nucleic acid, from compositions comprising encapsidated viral nucleic acid and viral particles that lack viral nucleic acid; methods for reducing the particle:genome ratio in a preparation of encapsidated viral nucleic acid; and methods for selectively inactivating viral particles that lack viral nucleic acid in a liquid composition comprising encapsidated viral nucleic acid and the viral particles that lack viral nucleic acid. The methods generally involve subjecting the composition to hydrostatic pressure such that the viral particles lacking viral nucleic acid are selectively inactivated.
摘要翻译： 本发明提供了从包含病毒核酸的组合物和缺乏病毒核酸的病毒颗粒中提取包封病毒，例如包含病毒核酸的病毒颗粒的方法; 用于在包封的病毒核酸制剂中降低颗粒：基因组比例的方法; 以及用于选择性地灭活含有包被病毒核酸的液体组合物中缺乏病毒核酸的病毒颗粒和缺乏病毒核酸的病毒颗粒的方法。 方法通常包括使组合物经受静水压力，使得缺乏病毒核酸的病毒颗粒被选择性地失活。
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公开(公告)号：US07625570B1
公开(公告)日：2009-12-01
申请号：US11372552
申请日：2006-03-09
CPC分类号： C12N7/00 , C12N15/86 , C12N2750/14143 , C12N2750/14151
摘要： The present invention provides methods of purifying adeno-associated virus (AAV) from compositions comprising AAV and at least a second, non-AAV; and methods for selectively inactivating a non-adeno-associated virus (non-AAV) in a liquid composition comprising AAV and the non-AAV. The methods generally involve subjecting the composition to hydrostatic pressure such that the non-AAV is selectively inactivated.
摘要翻译： 本发明提供了从包含AAV和至少第二非AAV的组合物中纯化腺相关病毒（AAV）的方法; 以及用于在包含AAV和非AAV的液体组合物中选择性灭活非腺相关病毒（非AAV）的方法。 方法通常包括使组合物经受静水压力，使得非AAV被选择性地失活。
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公开(公告)号：US20070254352A1
公开(公告)日：2007-11-01
申请号：US11739441
申请日：2007-04-24
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N15/86 , C12N2750/14143 , C12N2750/14151
摘要： The present invention provides methods of purifying encapsidated virus, e.g., viral particles comprising viral nucleic acid, from compositions comprising encapsidated viral nucleic acid and viral particles that lack viral nucleic acid; methods for reducing the particle:genome ratio in a preparation of encapsidated viral nucleic acid; and methods for selectively inactivating viral particles that lack viral nucleic acid in a liquid composition comprising encapsidated viral nucleic acid and the viral particles that lack viral nucleic acid. The methods generally involve subjecting the composition to hydrostatic pressure such that the viral particles lacking viral nucleic acid are selectively inactivated.
摘要翻译： 本发明提供了从包含病毒核酸的组合物和缺乏病毒核酸的病毒颗粒中提取包封病毒，例如包含病毒核酸的病毒颗粒的方法; 用于在包封的病毒核酸制剂中降低颗粒：基因组比例的方法; 以及用于选择性地灭活含有包被病毒核酸的液体组合物中缺乏病毒核酸的病毒颗粒和缺乏病毒核酸的病毒颗粒的方法。 方法通常包括使组合物经受静水压力，使得缺乏病毒核酸的病毒颗粒被选择性地失活。
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