公开(公告)号：US07968305B2

公开(公告)日：2011-06-28

申请号：US10104300

申请日：2002-03-22

Applicant: Xiaobo Wang ,  Lei Wu ,  Jun Quan Xu ,  Ming Xiang Huang ,  Weiping Yang ,  Jing Cheng ,  Jia Xu

Inventor： Xiaobo Wang ,  Lei Wu ,  Jun Quan Xu ,  Ming Xiang Huang ,  Weiping Yang ,  Jing Cheng ,  Jia Xu

IPC: G01N33/53

CPC classification number: G01N33/5005 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/049 ,  B03C5/026 ,  B03C5/028 ,  C12Q1/6869 ,  G01N15/1031 ,  G01N33/48728 ,  C12Q2565/629

Abstract: The present invention recognizes that the determination of ion transport function or property using direct detection methods, such as patch-clamps, whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and methods of use that allow for the direct analysis of ion transport function or property using microfabricated structures that can allow for automated detection of ion transport function or property. These biochips and methods of use thereof are particularly appropriate for automating the detection of ion transport function or property, particularly for screening purposes.

Abstract translation: 本发明认识到，利用间接检测方法（例如基于FRET的检测系统）的方法，优选使用直接检测方法（例如贴片钳，全细胞记录或单通道记录）的离子转运功能或性质的确定。 本发明提供了生物芯片和使用方法，其允许使用可以允许自动检测离子迁移功能或性质的微结构的直接分析离子转运功能或性质。 这些生物芯片及其使用方法特别适用于自动化离子迁移功能或性质的检测，特别是用于筛选目的。