Linker composition comprising silica-forming peptides

    公开(公告)号:US11633491B2

    公开(公告)日:2023-04-25

    申请号:US16793081

    申请日:2020-02-18

    IPC分类号: A61K47/65 A61K47/64

    摘要: The present disclosure relates to the use of silica-forming peptides (SFPs) Volp1 and Salp1 for protein fusion tags for protein purification, silica formation and self-encapsulation, and controlled release of biomolecules. After preparing a fusion protein containing the Volp1 or Salp1 peptide at the C-terminal of the protein, the adsorption conditions for silica (SiO2) surface were optimized. As a result, a high-purity fusion protein could be purified without an additional tag for purification. The fusion protein containing the silica-forming peptide was self-encapsulated and stably immobilized in the silica matrix through reaction with a silica precursor. The Volp1 and Salp1 peptides showed stronger adsorption ability for silica than the previously known R5 peptide. Using these characteristics, a silica complex wherein two proteins are encapsulated was prepared by mixing the R5 fusion protein with the Volp1 or Salp1 fusion protein. The resulting controlled release system allows the release of the R5 fusion protein in the first step and release of the Salp1 fusion protein in the second step based on the difference in adsorption affinity.

    COMPLEX LIGHT MODULATOR, HOLOGRAPHIC DISPLAY APPARATUS, AND METHOD OF GENERATING HOLOGRAM PATTERN

    公开(公告)号:US20220299937A1

    公开(公告)日:2022-09-22

    申请号:US17574811

    申请日:2022-01-13

    IPC分类号: G03H1/00 G03H1/02 G03H1/22

    摘要: Provided is a complex light modulator including a phase modulator, and an amplitude modulator, wherein the phase modulator and the amplitude modulator are configured to generate a first complex pixel having first complex light modulation characteristics and a second complex pixel having second complex light modulation characteristics, wherein the first complex pixel includes first sub-complex pixels that are provided in a 3×3 format and are configured to implement the first complex light modulation characteristics, wherein the second complex pixel includes second sub-complex pixels that are provided in a 3×3 format and are configured to implement the second complex light modulation characteristics, and wherein the first complex pixel and the second complex pixel respectively include a first overlapping pixel that is included in the first sub-complex pixels and the second sub-complex pixels.