公开(公告)号：US20110151467A1

公开(公告)日：2011-06-23

申请号：US13059703

申请日：2009-08-31

申请人： Kanako Usui ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

发明人： Kanako Usui ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

IPC分类号： C12Q1/68 ,  C12N9/10

CPC分类号： C12N15/1096 ,  C12Q1/6848 ,  C12Q2563/179 ,  C12Q2561/113 ,  C12Q2521/301 ,  C12Q2521/107

摘要： A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.

摘要翻译： 用于扩增通过逆转录反应合成的cDNA并检测作为逆转录反应的模板的RNA的组合物，所述组合物含有热稳定的DNA聚合酶，热稳定性核糖核酸酶H和嵌入染料。 由于本发明的组合物可以抑制用作cDNA合成模板的RNA对核酸扩增反应的影响，因此该组合物可用于RNA的检测，并且更有用于定量具有所需序列的RNA 实时RT-PCR。

公开(公告)号：US20080171337A1

公开(公告)日：2008-07-17

申请号：US12013033

申请日：2008-01-11

申请人： Koji MIYAZAKI ,  Akihisa Nakajima ,  Kanako Usui ,  Fumitsugu HINO ,  HIroyuki MUKAI ,  Ikunoshin KATO

发明人： Koji MIYAZAKI ,  Akihisa Nakajima ,  Kanako Usui ,  Fumitsugu HINO ,  HIroyuki MUKAI ,  Ikunoshin KATO

IPC分类号： C12Q1/68 ,  C12N13/00 ,  C12M1/42

CPC分类号： C12N15/1013

摘要： A method for isolating a nucleic acid from a cell-containing sample by using a magnet with a removable cover attached thereto and a plurality of containers arranged on a table, the plurality of containers having (1) a first container for mixing a cell-containing sample and magnetic beads to which cells can be adhered, (2) a cleaning tank for cleaning the magnetic beads with cells adhering thereto, and (3) a second container for heating the cleaned magnetic beads, the method includes: mixing a liquid including cell-containing sample and magnetic beads in the cell-containing sample in the first container so as to adhere cells in the cell-containing sample to magnetic beads; taking out the magnetic beads from the first container and cleaning the magnetic beads; and suspending the magnetic beads in a re-suspension buffer in the second container and heating the second container at 80 to 120° C. for 20 to 30°.

摘要翻译： 一种通过使用附着有可移除盖的磁体和布置在桌子上的多个容器从含细胞样品中分离核酸的方法，所述多个容器具有（1）第一容器，用于混合含细胞的 可以粘附细胞的样品和磁珠，（2）用于清洁附着有细胞的磁珠的清洗槽，和（3）用于加热清洁的磁珠的第二容器，该方法包括： 在第一容器中的含细胞样品中的样品和磁珠，以将细胞含有样品中的细胞粘附到磁珠上; 从第一容器中取出磁珠并清洁磁珠; 并将磁珠悬浮在第二容器中的再悬浮缓冲液中，并将第二容器在80至120℃下加热20至30℃。

公开(公告)号：US20110278229A1

公开(公告)日：2011-11-17

申请号：US13106252

申请日：2011-05-12

申请人： Kanako USUI ,  Sayuri KISHIDA ,  Tomomi TANAKA ,  Hiroyuki MUKAI

发明人： Kanako USUI ,  Sayuri KISHIDA ,  Tomomi TANAKA ,  Hiroyuki MUKAI

IPC分类号： B01D24/32 ,  C08L5/12 ,  C08J5/20 ,  B01D15/36

CPC分类号： C12N15/1003

摘要： A centrifugation method for separating a high specific gravity substance and a low specific gravity substance from a mixture thereof, which prevents the contamination of the high specific gravity substance in collecting the low specific gravity substance after a separation operation, is provided. The centrifugation method includes centrifuging a mixture comprising a high specific gravity substance, a low specific gravity substance, and at least one of a thermoreversible gel and a dissolved gelling agent capable of forming a thermoreversible gel, in which the thermoreversible gel or the dissolved gelling agent forms a gel layer which separates the low specific gravity substance and the high specific gravity substance. The centrifugation is preferably carried out at a temperature equal to or lower than the gelation temperature of the gelling agent.

摘要翻译： 提供了一种用于从其混合物中分离高比重物质和低比重物质的离心方法，其防止在分离操作之后收集低比重物质时高比重物质的污染。 离心方法包括将包含高比重物质，低比重物质和能够形成热可逆凝胶的热可逆凝胶和溶解胶凝剂中的至少一种的混合物离心，其中热可逆凝胶或溶解的胶凝剂 形成分离低比重物质和高比重物质的凝胶层。 离心优选在等于或低于胶凝剂凝胶化温度的温度下进行。

公开(公告)号：US10760074B2

公开(公告)日：2020-09-01

申请号：US13059703

申请日：2009-08-31

申请人： Kanako Usui ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

发明人： Kanako Usui ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12Q1/6848

摘要： A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.