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    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
    2.
    发明授权
    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays 有权
    多分析物均匀荧光免疫测定的装置和方法

    公开(公告)号：US06316274B1

    公开(公告)日：2001-11-13

    申请号：US09516307

    申请日：2000-03-01

    申请人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin Caldwell ,  Vera Janatová ,  Shao-Chie Huang

    发明人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin Caldwell ,  Vera Janatová ,  Shao-Chie Huang

    IPC分类号： G01N33543

    CPC分类号： G01N21/6452 ,  C07C281/02 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N21/7703 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807

    摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.

    摘要翻译： 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用具有整体半柱面透镜的平面波导，并具有多分析物特征和校准特征，以及改善的消逝场强度。 生物传感器和测定方法的优选实施方案具有每个针对不同分析物特异性的捕获分子的斑块，其邻近设置在单个贮存器内。 捕获分子通过捕获分子上的硫醇基团的特异性偶联而固定在波导表面上的贴片上，而光致亲和性交联剂又连接到波导表面或非特异性结合抗性涂层上 表面。 通过在涉及光亲和性交联剂的涉及掩模，激光光源等的选择性照射的过程中选择性地照射波导表面的一部分来产生不同抗体的斑块。

    Methods and compositions for modifying the surface of a polymeric substrate
    4.
    发明授权
    Methods and compositions for modifying the surface of a polymeric substrate 失效
    用于改性聚合物基材表面的方法和组合物

    公开(公告)号：US06706161B1

    公开(公告)日：2004-03-16

    申请号：US09095493

    申请日：1998-06-10

    申请人： Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    发明人： Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    IPC分类号： G01N2726

    CPC分类号： C08J7/047 ,  C08G65/329 ,  C08G65/33303 ,  C08G65/334 ,  C08J2471/00 ,  C09D171/02 ,  C25D13/22

    摘要： Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of a bout 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

    摘要翻译： 提供了改性聚合物基材表面的方法和组合物。 在本方法中，聚合物基材的表面的至少一部分与生物结垢的表面活性剂组合物接触。 组合物的表面活性剂分子的至少一部分用在反应2-12的pH范围内电荷稳定的非反应性带电端基末端修饰。本发明的方法和组合物特别用于电泳装置， 如毛细管电泳装置。

    Hydrogels of water soluble polymers crosslinked by protein domains
    5.
    发明授权
    Hydrogels of water soluble polymers crosslinked by protein domains 有权
    由蛋白质结构域交联的水溶性聚合物的水凝胶

    公开(公告)号：US07179487B1

    公开(公告)日：2007-02-20

    申请号：US09336259

    申请日：1999-06-18

    申请人： Jindrich Kopecek ,  Russell Stewart ,  Karin Caldwell ,  Chung Wang ,  Chih-Hu Ho

    发明人： Jindrich Kopecek ,  Russell Stewart ,  Karin Caldwell ,  Chung Wang ,  Chih-Hu Ho

    IPC分类号： A61K9/00 ,  A61K38/03 ,  A61K38/16

    CPC分类号： A61K9/06 ,  A61K38/00 ,  A61K47/42

    摘要： A stimuli-responsive, hybrid hydrogel wherein the bulk of the polymer is made up of relatively inexpensive water soluble polymer strands crosslinked by protein domains. The responsiveness of the gel is controlled or modulated by the protein component.The physical and biological properties of the hydrogel are determined by specifically designed or engineered protein domains.The crosslinking of the protein domains to the water soluble polymers is by means of non-covalent bonding such as chelation or coordination bonding, biotin-avidin bonding, protein—protein interaction and protein-ligand interaction, or by means of covalent bonding. Methods of making and using the polymer-protein hydrogels are disclosed in this application.

    摘要翻译： 一种刺激响应性混合型水凝胶，其中大部分聚合物由相对便宜的由蛋白质结构域交联的水溶性聚合物链构成。 凝胶的响应性由蛋白质组分控制或调节。 水凝胶的物理和生物学性质通过专门设计或改造的蛋白质结构域来确定。 蛋白质结构域与水溶性聚合物的交联是通过非共价键合，例如螯合或配位键合，生物素 - 抗生物素蛋白键合，蛋白质 - 蛋白质相互作用和蛋白质 - 配体相互作用，或通过共价键。 在本申请中公开了制备和使用聚合物 - 蛋​​白质水凝胶的方法。

    Methods and compositions for modifying the surface of a polymeric substrate
    8.
    发明授权
    Methods and compositions for modifying the surface of a polymeric substrate 失效
    用于改性聚合物基材表面的方法和组合物

    公开(公告)号：US07201834B2

    公开(公告)日：2007-04-10

    申请号：US10386581

    申请日：2003-03-11

    申请人： Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    发明人： Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    IPC分类号： G01N27/453

    CPC分类号： C08J7/047 ,  C08G65/329 ,  C08G65/33303 ,  C08G65/334 ,  C08J2471/00 ,  C09D171/02 ,  C25D13/22

    摘要： Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

    摘要翻译： 提供了改性聚合物基材表面的方法和组合物。 在本方法中，聚合物基材的表面的至少一部分与生物结垢的表面活性剂组合物接触。 组合物的表面活性剂分子的至少一部分用在约2至12的pH范围内电荷稳定的非反应性带电端基末端修饰。本发明方法和组合物特别用于电泳装置，例如 作为毛细管电泳装置。

    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
    9.
    发明授权
    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays 失效
    多分析物均匀荧光免疫测定的装置和方法

    公开(公告)号：US06979567B2

    公开(公告)日：2005-12-27

    申请号：US10008339

    申请日：2001-11-13

    申请人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin Caldwell ,  Vera Janatová ,  Shao-Chie Huang

    发明人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin Caldwell ,  Vera Janatová ,  Shao-Chie Huang

    IPC分类号： C07C281/02 ,  C08G65/329 ,  G01N21/25 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  C12M3/00

    CPC分类号： G01N21/6452 ,  C07C281/02 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N21/7703 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807

    摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semicylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method has patches of capture molecules, each specific for a different analyte disposed adjacently within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers, which in turn are coupled to the waveguide surface or to a nonspecific binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers, the selective irradiation involving a mask, a laser light source, or the like.

    摘要翻译： 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用具有整体半圆柱透镜的平面波导，并具有多分析物特征和校准特征，以及改善的消逝场强度。 生物传感器和测定方法的优选实施方案具有捕获分子的斑块，每个捕获分子具有针对在单个贮存器内相邻设置的不同分析物的特征。 捕获分子通过捕获分子上的硫醇基团的特异性偶联而固定在波导表面上的贴片上，而光致亲和交联剂依次耦合到波导表面或表面上的非特异性结合抗性涂层 。 在偶联光亲和性交联剂，涉及掩模的选择性照射，激光光源等的过程中，通过选择性地照射波导表面的一部分来产生不同抗体的贴片。