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    Triazine derivatives as universal peptide isotope tag reagents (U-PIT)
    1.
    发明授权
    Triazine derivatives as universal peptide isotope tag reagents (U-PIT) 失效
    三嗪衍生物作为通用肽同位素标签试剂（U-PIT）

    公开(公告)号：US07425451B2

    公开(公告)日：2008-09-16

    申请号：US10318845

    申请日：2002-12-13

    Applicant: Karla M. Robotti ,  James Alexander Apffel, Jr.

    Inventor： Karla M. Robotti ,  James Alexander Apffel, Jr.

    IPC: G01N33/00 ,  C07D403/00 ,  A01N43/66 ,  A61K31/53

    CPC classification number: C07D403/12 ,  C07D401/14

    Abstract: Compounds, compositions, methods for sequencing proteins and peptides, and methods for identifying proteins and peptides in a mixture, are disclosed. Compounds of formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety capable of being isotopically labeled, and C is a charge replacement group, are used to label the peptides at the N-terminus or the C-terminus. The tagged peptides can then be analyzed by mass spectroscopy.

    Abstract translation: 公开了用于测定蛋白质和肽的化合物，组合物，方法以及用于鉴定混合物中的蛋白质和肽的方法。 其中A是亲核反应性基团的式A-B-C化合物，B是能够同位素标记的可检测部分，C是电荷取代基，用于在N末端或C末端标记肽。 然后可以通过质谱法分析标记的肽。

    Evanescent wave sensor with attached ligand
    2.
    发明授权
    Evanescent wave sensor with attached ligand 有权
    具有附着配体的渐逝波传感器

    公开(公告)号：US07396676B2

    公开(公告)日：2008-07-08

    申请号：US11143358

    申请日：2005-05-31

    Applicant: Karla M. Robotti ,  Daniel B. Roitman

    Inventor： Karla M. Robotti ,  Daniel B. Roitman

    IPC: C12M1/34

    CPC classification number: G01N21/553 ,  G01N33/54353 ,  Y10S435/808 ,  Y10S436/805

    Abstract: The invention in particular embodiments provides an evanescent wave sensor which includes a ligand bound to a sensor substrate via a NCYX linker moiety, as defined herein. Methods of making the subject evanescent wave sensors are also provided which include contacting a first reactive moiety which has an isocyanato (or isothiocyanato) moiety with a second reactive moiety which has an hydroxyl, thiol, or amino moiety, as further described herein. Also provided by the invention are methods in which a subject evanescent wave sensor is contacted with a sample, and binding of analytes in the sample to the sensor is assessed by evanescent wave detection. The invention also provides kits and systems for performing the subject methods.

    Abstract translation: 本发明在特定实施方案中提供了一种ev逝波传感器，其包括通过如本文所定义的NCYX接头部分与传感器底物结合的配体。 还提供了制备对象ev逝波传感器的方法，其包括使具有异氰酸酯基（或异硫氰酸酯基）部分的第一反应性部分与具有羟基，硫醇或氨基部分的第二反应性部分接触，如本文进一步描述的。 本发明还提供了一种方法，其中对象ev逝波传感器与样品接触，并且通过ev逝波检测来评估样品中的分析物与传感器的结合。 本发明还提供用于执行主题方法的套件和系统。

    Methods and compositions for modifying the surface of a polymeric substrate
    3.
    发明授权
    Methods and compositions for modifying the surface of a polymeric substrate 失效
    用于改性聚合物基材表面的方法和组合物

    公开(公告)号：US07201834B2

    公开(公告)日：2007-04-10

    申请号：US10386581

    申请日：2003-03-11

    Applicant: Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    Inventor： Sally A. Swedberg ,  Karla M. Robotti ,  Karin Caldwell

    IPC: G01N27/453

    CPC classification number: C08J7/047 ,  C08G65/329 ,  C08G65/33303 ,  C08G65/334 ,  C08J2471/00 ,  C09D171/02 ,  C25D13/22

    Abstract: Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

    Abstract translation: 提供了改性聚合物基材表面的方法和组合物。 在本方法中，聚合物基材的表面的至少一部分与生物结垢的表面活性剂组合物接触。 组合物的表面活性剂分子的至少一部分用在约2至12的pH范围内电荷稳定的非反应性带电端基末端修饰。本发明方法和组合物特别用于电泳装置，例如 作为毛细管电泳装置。

    Microfluidic Devices Comprising Fluid Flow Paths Having Monolithic Chromatographic Material
    5.
    发明申请
    Microfluidic Devices Comprising Fluid Flow Paths Having Monolithic Chromatographic Material 有权
    包含具有单片色谱材料的流体流路的微流体装置

    公开(公告)号：US20110028669A1

    公开(公告)日：2011-02-03

    申请号：US12902008

    申请日：2010-10-11

    Applicant: KARLA M. ROBOTTI

    Inventor： KARLA M. ROBOTTI

    IPC: C08F20/06 ,  C08F112/08

    CPC classification number: G01N30/6095 ,  G01N2030/528 ,  Y10T29/494 ,  Y10T156/10

    Abstract: Aspects of the invention include a microfluidic device having a fluid flow path and a monolithic chromatographic material covalently bound to at least a portion of a polymeric surface of the fluid flow path. Aspects of the invention further include methods of making and using such devices.

    Abstract translation: 本发明的方面包括具有流体流动路径和共价结合到流体流动路径的聚合物表面的至少一部分的整体色谱材料的微流体装置。 本发明的方面还包括制造和使用这些装置的方法。

    Enrichment and tagging of glycosylated proteins
    10.
    发明授权
    Enrichment and tagging of glycosylated proteins 失效
    糖基化蛋白的富集和标记

    公开(公告)号：US07384754B2

    公开(公告)日：2008-06-10

    申请号：US10699449

    申请日：2003-10-31

    Applicant: Karla M. Robotti

    Inventor： Karla M. Robotti

    IPC: G01N33/53 ,  G01N33/00 ,  C12P21/06

    CPC classification number: G01N33/50 ,  C07K1/1077 ,  C07K1/16 ,  G01N33/54306 ,  G01N2400/00 ,  Y10T436/141111

    Abstract: A method useful in the analysis of glycosylated proteins, in which a mixture containing glycosylated proteins and unglycosylated proteins is contacted with a resin that includes a nucleophile bound to a solid support via a linker. The contacting is performed under conditions sufficient to result in removal of the glycosyl group from the glycosylated proteins and to concomitantly result in the deglycosylated proteins covalently bound to the solid support. The deglycosylated proteins bound to the solid support may be rinsed to remove proteins that are not covalently bound to the solid support. The deglycosylated proteins are released from the solid support and may be subjected to further purification and/or analysis.

    Abstract translation: 用于分析糖基化蛋白质的方法，其中含有糖基化蛋白质和未糖基化蛋白质的混合物与包含通过接头与固体支持物结合的亲核试剂的树脂接触。 接触在足以导致从糖基化蛋白质中除去糖基的条件下进行，并且伴随地导致与固体支持物共价结合的去糖基化蛋白质。 可以漂洗与固体支持物结合的去糖基化蛋白质以去除不共价结合固体支持物的蛋白质。 去糖基化的蛋白质从固体支持物中释放，并可进行进一步的纯化和/或分析。

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