公开(公告)号：US20050114041A1

公开(公告)日：2005-05-26

申请号：US10974505

申请日：2004-10-26

申请人： Shady Gawad ,  Philippe Renaud ,  Karl Brander ,  Thomas Hessler

发明人： Shady Gawad ,  Philippe Renaud ,  Karl Brander ,  Thomas Hessler

IPC分类号： B01L3/00 ,  G01N15/10 ,  G01N15/12 ,  G06F19/00

CPC分类号： G01N15/1227 ,  B01L3/5027 ,  G01N15/1012 ,  G01N15/1056 ,  G01N2015/1254

摘要： A method for discrimination of particles, preferably biological cells, in a measurement channel having a liquid for transporting the particles by impedance spectroscopy. Pairs of measurement and reference electrodes are arranged in the measurement channel. During movement of a particle through the pair of measurement electrodes, the pairs of measurement and reference electrodes are admitted with same input signals having different frequencies. Measurement values at the measurement and reference electrodes are compared to determine particle specific values for the particle being moved through the measurement channel. The particle specific values for the different frequencies are normalized to a particle specific basic value at a basic frequency; and then the normalized particle specific values are compared with corresponding values of at least one reference particle at the same different frequencies. The comparison shows changes in the capacitance or in the conductance of the particle, which are used in discriminating the particle.

摘要翻译： 在具有用于通过阻抗光谱传输颗粒的液体的测量通道中鉴别颗粒，优选生物细胞的方法。 一对测量和参考电极排列在测量通道中。 在粒子通过该对测量电极移动期间，测量和参考电极对允许具有不同频率的相同输入信号。 比较测量和参考电极的测量值，以确定通过测量通道移动的粒子的粒子特定值。 将不同频率的粒子特定值归一化为基本频率下的粒子特定基准值; 然后将归一化的粒子特定值与相同不同频率的至少一个参考粒子的相应值进行比较。 比较显示用于区分颗粒的电容或电导率的变化。

公开(公告)号：US08105820B2

公开(公告)日：2012-01-31

申请号：US10584484

申请日：2004-11-15

申请人： Karl Brander ,  Louis Tiefenauer

发明人： Karl Brander ,  Louis Tiefenauer

IPC分类号： C12M3/00

CPC分类号： G01N33/68 ,  B82Y5/00 ,  G01N33/54366 ,  G01N33/6845

摘要： An assay chip for investigation the functionality of membrane proteins and their interactions with molecules includes a nanopore array having a plurality of nanopores in a suitable support layer deposited on the nanopore substrate and being a substantially planar support layer having a plurality of nanopores corresponding to the nanopores of the nanopore substrate. The chip includes further a biologically effective layer capable to host at least a non-lipid molecule or functional molecule, deposited on the support layer and covering the plurality of nanopores, resulting in accesible nanopores from both sides of the biologically effective layer for measurement or imaging. The structured support allows generating a biologically effective membrane, such as a lipid bilayer membrane, having high and reliable stability in a manner that its fluidity is sustained in order to keep integrated membrane proteins in the lipid bilayer in its full biological functionality.

摘要翻译： 用于研究膜蛋白的功能及其与分子的相互作用的测定芯片包括纳米孔阵列，该纳米孔阵列在沉积在纳米孔基底上的合适的支持层中具有多个纳米孔，并且是具有对应于纳米孔的多个纳米孔的基本平坦的支撑层 的纳米孔基材。 该芯片还包括能够承载沉积在支持层上并覆盖多个纳米孔的至少一种非脂质分子或功能性分子的生物学有效层，从而生物学有效层两侧的可接受的纳米孔用于测量或成像 。 结构化支持物允许产生生物学有效的膜，例如脂质双层膜，其以保持其流动性的方式具有高且可靠的稳定性，以将整合的膜蛋白保持在脂质双层中具有其完整的生物学功能。

公开(公告)号：US20070275480A1

公开(公告)日：2007-11-29

申请号：US10584484

申请日：2004-11-15

申请人： Karl Brander ,  Louis Tiefenauer

发明人： Karl Brander ,  Louis Tiefenauer

IPC分类号： G01N33/68 ,  G01N33/566

CPC分类号： G01N33/68 ,  B82Y5/00 ,  G01N33/54366 ,  G01N33/6845

摘要： An assay chip for investigation the functionality of membrane proteins and their interactions with molecules includes a nanopore array having a plurality of nanopores in a suitable support layer deposited on the nanopore substrate and being a substantially planar support layer having a plurality of nanopores corresponding to the nanopores of the nanopore substrate. The chip includes further a biologically effective layer capable to host at least a non-lipid molecule or functional molecule, deposited on the support layer and covering the plurality of nanopores, resulting in accesible nanopores from both sides of the biologically effective layer for measurement or imaging. The structured support allows generating a biologically effective membrane, such as a lipid bilayer membrane, having high and reliable stability in a manner that its fluidity is sustained in order to keep integrated membrane proteins in the lipid bilayer in its full biological functionality.

摘要翻译： 用于研究膜蛋白的功能及其与分子的相互作用的测定芯片包括纳米孔阵列，该纳米孔阵列在沉积在纳米孔基底上的合适的支持层中具有多个纳米孔，并且是具有对应于纳米孔的多个纳米孔的基本平坦的支撑层 的纳米孔基材。 该芯片还包括能够承载沉积在支持层上并覆盖多个纳米孔的至少一种非脂质分子或功能性分子的生物学有效层，从而生物学有效层两侧的可接受的纳米孔用于测量或成像 。 结构化支持物允许产生生物学有效的膜，例如脂质双层膜，其以保持其流动性的方式具有高且可靠的稳定性，以将整合的膜蛋白保持在脂质双层中具有其完整的生物学功能。