公开(公告)号：US20050114041A1

公开(公告)日：2005-05-26

申请号：US10974505

申请日：2004-10-26

申请人： Shady Gawad ,  Philippe Renaud ,  Karl Brander ,  Thomas Hessler

发明人： Shady Gawad ,  Philippe Renaud ,  Karl Brander ,  Thomas Hessler

IPC分类号： B01L3/00 ,  G01N15/10 ,  G01N15/12 ,  G06F19/00

CPC分类号： G01N15/1227 ,  B01L3/5027 ,  G01N15/1012 ,  G01N15/1056 ,  G01N2015/1254

摘要： A method for discrimination of particles, preferably biological cells, in a measurement channel having a liquid for transporting the particles by impedance spectroscopy. Pairs of measurement and reference electrodes are arranged in the measurement channel. During movement of a particle through the pair of measurement electrodes, the pairs of measurement and reference electrodes are admitted with same input signals having different frequencies. Measurement values at the measurement and reference electrodes are compared to determine particle specific values for the particle being moved through the measurement channel. The particle specific values for the different frequencies are normalized to a particle specific basic value at a basic frequency; and then the normalized particle specific values are compared with corresponding values of at least one reference particle at the same different frequencies. The comparison shows changes in the capacitance or in the conductance of the particle, which are used in discriminating the particle.

摘要翻译： 在具有用于通过阻抗光谱传输颗粒的液体的测量通道中鉴别颗粒，优选生物细胞的方法。 一对测量和参考电极排列在测量通道中。 在粒子通过该对测量电极移动期间，测量和参考电极对允许具有不同频率的相同输入信号。 比较测量和参考电极的测量值，以确定通过测量通道移动的粒子的粒子特定值。 将不同频率的粒子特定值归一化为基本频率下的粒子特定基准值; 然后将归一化的粒子特定值与相同不同频率的至少一个参考粒子的相应值进行比较。 比较显示用于区分颗粒的电容或电导率的变化。

公开(公告)号：US07294249B2

公开(公告)日：2007-11-13

申请号：US10355320

申请日：2003-01-31

申请人： Shady Gawad ,  Martin Wüthrich ,  Philippe Renaud

发明人： Shady Gawad ,  Martin Wüthrich ,  Philippe Renaud

IPC分类号： G01N27/02 ,  G01F1/64

CPC分类号： G01N15/1056 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2400/0415 ,  B03C5/026 ,  G01N15/1031 ,  G01N27/02 ,  G01N2015/1006

摘要： Microtechnologically prepared component as a flow cytometer. The component contains a preparation area to specifically influence and separate the particles, preferably by dielectrophoresis, a measuring channel area for characterizing the particles, and a sorting area for sorting the particles identified in the measuring channel area by dielectrophoresis. The sorting includes switching elements which permit active guidance of the particles into two or more subchannels corresponding to the criteria which have been registered in the measuring channel area. With a component configured in this way for the use of a flow cytometer, quick and precise sorting of particles, in particular biological cells in a suspension, can be implemented.

摘要翻译： 微技术制备的组分作为流式细胞仪。 组分包含用于特别影响和分离颗粒的制备区域，优选通过介电电泳，用于表征颗粒的测量通道区域和用于通过介电电泳分选在测量通道区域中识别的颗粒的分选区域。 排序包括允许主动地将颗粒引导到对应于已经登记在测量通道区域中的标准的两个或更多个子通道中的开关元件。 通过以这种方式配置以使用流式细胞仪的组件，可以实现对悬浮液中的颗粒，特别是生物细胞的快速和精确的分选。